Detection of Cryptosporidium in miniaturised fluidic devices

Bridle, Helen; Kersaudy-Kerhoas, Maïwenn; Miller, Brian; Gavriilidou, Despoina; Katzer, Frank; Innes, Elisabeth A.; Desmulliez, Marc Phillipe Yves

doi:10.1016/j.watres.2012.01.010

Cited by 51 publications

(30 citation statements)

References 83 publications

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“…With fewer than 10 oocysts capable of initiating a significant human infection 13, 14 , waterborne monitoring for the presence of this pathogen is therefore essential 15, 16 . The standard method of isolation and detection for Cryptosporidium oocysts present in treated water supplies, EPA 1623.1 17 , typically involves the processing of 50–100 L of water ( e.g ., on site) or 1000 L ( e.g ., at the treatment works) to a 50 µL volume, before highly-skilled microscopists confirm detection using specialised techniques 18 . This process also simultaneously recovers cysts of the Giardia genus – protozoan parasites that may also cause gastroenteritis if ingested by humans 17 .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

McGrath

Honrado

Spencer

et al. 2017

Sci Rep

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At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.

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Section: Introductionmentioning

confidence: 99%

Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

McGrath

Honrado

Spencer

et al. 2017

Sci Rep

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“…Most applications in clinical diagnostics and environmental monitoring demand robust, cost-effective detection devices for the rapid and sensitive analysis of analytes. For instance, effective surveillance for waterborne pathogens could be achieved by developing low-cost, portable sensors for the real-time detection of only 10–100 organisms in a sample [6–8]. …”

Section: Introductionmentioning

confidence: 99%

Recent Developments in Optical Detection Technologies in Lab-on-a-Chip Devices for Biosensing Applications

Pires

Dong

Hanke

et al. 2014

Sensors

247

148

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The field of microfluidics has yet to develop practical devices that provide real clinical value. One of the main reasons for this is the difficulty in realizing low-cost, sensitive, reproducible, and portable analyte detection microfluidic systems. Previous research has addressed two main approaches for the detection technologies in lab-on-a-chip devices: (a) study of the compatibility of conventional instrumentation with microfluidic structures, and (b) integration of innovative sensors contained within the microfluidic system. Despite the recent advances in electrochemical and mechanical based sensors, their drawbacks pose important challenges to their application in disposable microfluidic devices. Instead, optical detection remains an attractive solution for lab-on-a-chip devices, because of the ubiquity of the optical methods in the laboratory. Besides, robust and cost-effective devices for use in the field can be realized by integrating proper optical detection technologies on chips. This review examines the recent developments in detection technologies applied to microfluidic biosensors, especially addressing several optical methods, including fluorescence, chemiluminescence, absorbance and surface plasmon resonance.

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“…polymerase chain reaction (PCR) [5][6][7][8][9][10][11][12] . These methods give results within hours rather than days, as is the case for conventional detection method (i.e.…”

Section: Introductionmentioning

confidence: 99%

Field portable mobile phone based fluorescence microscopy for detection ofGiardia lambliacysts in water samples

Koydemir

Gӧrӧcs

McLeod³

et al. 2015

SPIE Proceedings

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Giardia lamblia is a waterborne parasite that causes an intestinal infection, known as giardiasis, and it is found not only in countries with inadequate sanitation and unsafe water but also streams and lakes of developed countries. Simple, sensitive, and rapid detection of this pathogen is important for monitoring of drinking water. Here we present a costeffective and field portable mobile-phone based fluorescence microscopy platform designed for automated detection of Giardia lamblia cysts in large volume water samples (i.e., 10 ml) to be used in low-resource field settings. This fluorescence microscope is integrated with a disposable water-sampling cassette, which is based on a flow-through porous polycarbonate membrane and provides a wide surface area for fluorescence imaging and enumeration of the captured Giardia cysts on the membrane. Water sample of interest, containing fluorescently labeled Giardia cysts, is introduced into the absorbent pads that are in contact with the membrane in the cassette by capillary action, which eliminates the need for electrically driven flow for sample processing. Our fluorescence microscope weighs ~170 grams in total and has all the components of a regular microscope, capable of detecting individual fluorescently labeled cysts under light-emitting-diode (LED) based excitation. Including all the sample preparation, labeling and imaging steps, the entire measurement takes less than one hour for a sample volume of 10 ml. This mobile phone based compact and costeffective fluorescent imaging platform together with its machine learning based cyst counting interface is easy to use and can even work in resource limited and field settings for spatio-temporal monitoring of water quality.

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Detection of Cryptosporidium in miniaturised fluidic devices

Cited by 51 publications

References 83 publications

Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

Recent Developments in Optical Detection Technologies in Lab-on-a-Chip Devices for Biosensing Applications

Field portable mobile phone based fluorescence microscopy for detection ofGiardia lambliacysts in water samples

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