Detection of CpG island hypermethylation of caspase‐8 in neuroblastoma using an oligonucleotide array

Kamimatsuse, Arata; Matsuura, Kaoru; Moriya, Shogo; Fukuba, Ikuko; Yamaoka, Hiroaki; Fukuda, Emi; Kamei, Naosuke; Hiyama, Keiko; Sueda, Taijiro; Hiyama, Eiso

doi:10.1002/pbc.21977

Cited by 9 publications

(7 citation statements)

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“…Since decreased expression of caspase 8 is a recurrent event in NB [18] , we tested whether CASP8 SNP D302H could affect the expression of caspase 8. We measured caspase 8 mRNA expression in two independent cohorts of NB tumor samples.…”

Section: Resultsmentioning

confidence: 99%

CASP8 SNP D302H (rs1045485) Is Associated with Worse Survival in MYCN-Amplified Neuroblastoma Patients

et al. 2014

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BackgroundNeuroblastoma is a pediatric cancer that exhibits a wide clinical spectrum ranging from spontaneous regression in low-risk patients to fatal disease in high-risk patients. The identification of single nucleotide polymorphisms (SNPs) may help explain the heterogeneity of neuroblastoma and assist in identifying patients at higher risk for poor survival. SNPs in the TP53 pathway are of special importance, as several studies have reported associations between TP53 pathway SNPs and cancer. Of note, less than 2% of neuroblastoma tumors have a TP53 mutation at diagnosis.Patients and MethodsWe selected 21 of the most frequently studied SNPs in the TP53 pathway and evaluated their association with outcome in 500 neuroblastoma patients using TaqMan allelic discrimination assays.Results and ConclusionWe investigated the impact of 21 SNPs on overall survival, event-free survival, age at diagnosis, MYCN status, and stage of the disease in 500 neuroblastoma patients. A missense SNP in exon 10 of the CASP8 gene SNP D302H was associated with worse overall and event-free survival in patients with MYCN-amplified neuroblastoma tumors.

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Section: Resultsmentioning

confidence: 99%

CASP8 SNP D302H (rs1045485) Is Associated with Worse Survival in MYCN-Amplified Neuroblastoma Patients

et al. 2014

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“…Most commonly in NB as well as other cancers, casp8 repression is a result of hypermethylation at CpG islands within the CASP8 promoter region [117,121,123]. Methylation of the promoter region regulates casp8 expression thus allowing, based on cellular conditions and signaling, for casp8 re-expression.…”

Section: Discussionmentioning

confidence: 99%

“…Teitz et al and others have demonstrated that casp8 is preferentially silenced in NB cell lines and patient samples though rarely deleted [17,[115][116][117]. Loss of casp8 has also been shown in other tumors including small cell lung carcinoma, rhabdomyosarcoma, glioblastoma, retinoblastoma, medulloblastoma, Wilms tumors and colorectal carcinomas [118][119][120][121][122][123].…”

Section: Caspase-8 In Cancermentioning

confidence: 99%

“…Other tumors including medulloblastoma, retinoblastoma, rhabdomyosarcoma, and small cell lung carcinoma have also been shown to silence casp8 expression [118,119,121]. Downregulation of casp8 is frequently attributed to the hypermethylation of CpG islands within the CASP8 promoter region [117]. Research suggests that the loss of casp8 in NB significantly enhanced tumor cell metastasis and survival at secondary sites by inhibiting integrin-mediated cell death (IMD) [88,145,156].…”

Section: Chapter 2 Caspase-8 Promotes Neuroblastoma Tumorigenesis Inmentioning

confidence: 99%

“…Caspase-8, located on chromosome 2q33-34, is the initiator caspase of the extrinsic apoptotic signaling pathway [28,117]. Caspase-8 is composed of a proteinprotein interacting N-terminal domain and a catalytic C-terminal domain; comprised of a large and small subunit separated by a short linker sequence [36].…”

Section: Chapter 2 Caspase-8 Promotes Neuroblastoma Tumorigenesis Inmentioning

confidence: 99%

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The Protumorigenic Role of Caspase-8 in Neuroblastoma

Twitchell¹

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DEDICATIONThis dissertation is dedicated to my wonderful parents who have given immeasurable love, devotion, guidance, and sacrifice throughout the years on my behalf and have helped to make this achievement possible.A special dedication is extended to my lovely wife, who without her, none of this would have been possible. Through her unselfish love, devotion, and personal sacrifice for me and our family, she has been my strength, my support, and my motivation to endure when times got hard. To my wonderful children (Camryn, Leigha, Ethan, McKenna, and Hayden) who have kept me focused on what matters most in life.iv ACKNOWLEDGEMENTS This work would not have been possible without the mentorship of Dr. Jill Lahti, who lost her battle to breast cancer. Dr. Jill Lahti was a great mentor who allowed a young scientist, such as me, to explore and develop my technique, logic, and scientific reasoning. I regret that she could not be here to see this work completed. I appreciate her willingness to accept me into her lab and work on her project as my own. She was always willing to listen to my ideas and theories, no matter how extravagant, and always encouraged me to explore many of those ideas.Special thanks are extended to my current mentor Dr. Gerard Zambetti who was gracious enough to adopt me into his lab. I appreciate all the work he did behind the scenes to keep me at St. Jude long enough to complete my dissertation. Dr. Zambetti is an incredible mentor who is constantly urging me to challenge my hypothesis and myself as a researcher. He has given me great insight and understanding as to how be a good scientist, constantly questioning and challenging the hypothesis. Dr. Zambetti instilled in me a stronger desire to improve as a scientist each time we met. I am also very

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Defective Apoptosis Signaling in Cancer

Johnson

2012

Cell Death Signaling in Cancer Biology and Treatment

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Detection of CpG island hypermethylation of caspase‐8 in neuroblastoma using an oligonucleotide array

Abstract: A significant difference in the methylation status within the CpG island of CASP8 was shown between favorable and unfavorable subtypes, and CASP8 methylation detected by oligoarray may be useful in the clinical evaluation of neuroblastomas.

Cited by 9 publications

References 23 publications

CASP8 SNP D302H (rs1045485) Is Associated with Worse Survival in MYCN-Amplified Neuroblastoma Patients

CASP8 SNP D302H (rs1045485) Is Associated with Worse Survival in MYCN-Amplified Neuroblastoma Patients

The Protumorigenic Role of Caspase-8 in Neuroblastoma

Defective Apoptosis Signaling in Cancer

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