Detection of Coronavirus Infection of Man by Immunofluorescence

Monto, Arnold S.; Rhodes, Lenore M

doi:10.3181/00379727-155-39761

Cited by 23 publications

(18 citation statements)

References 7 publications

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“…Alarmingly, SARS-CoV infections appear to stimulate cross-reactive antibody responses to other human coronaviruses, including HCoV-NL63 [77]. Such cross reaction between human coronaviruses has been reported previously for immunoflourescence and complement fixation tests [78,79]. In fact, false positive results were also reported for SARS-CoV detection using ELISA tests based on the recombinant N antigen [80].…”

Section: Antigen and Antibody Detection Assaysmentioning

confidence: 72%

Understanding Human Coronavirus HCoV-NL63~!2009-11-13~!2010-04-09~!2010-05-25~!

Abdul-Rasool¹,

Fielding²

2010

TOVJ

145

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Even though coronavirus infection of humans is not normally associated with severe diseases, the identification of the coronavirus responsible for the outbreak of severe acute respiratory syndrome showed that highly pathogenic coronaviruses can enter the human population. Shortly thereafter, in Holland in 2004, another novel human coronavirus (HCoV-NL63) was isolated from a seven-month old infant suffering from respiratory symptoms. This virus has subsequently been identified in various countries, indicating a worldwide distribution. HCoV-NL63 has been shown to infect mainly children and the immunocommpromised, who presented with either mild upper respiratory symptoms (cough, fever and rhinorrhoea) or more serious lower respiratory tract involvement such as bronchiolitis and croup, which was observed mainly in younger children. In fact, HCoV-NL63 is the aetiological agent for up to 10% of all respiratory diseases. This review summarizes recent findings of human coronavirus HCoV-NL63 infections, including isolation and identification, phylogeny and taxonomy, genome structure and transcriptional regulation, transmission and pathogenesis, and detection and diagnosis.

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Section: Antigen and Antibody Detection Assaysmentioning

confidence: 72%

Understanding Human Coronavirus HCoV-NL63~!2009-11-13~!2010-04-09~!2010-05-25~!

Abdul-Rasool¹,

Fielding²

2010

TOVJ

145

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“…It has also been previously reported that HCV-229 E appeared to be antigenically unrelated to MHV-3 and HCV-OC43 (11 ). The lack of relationship by immunofluorescence of HCV-0C 43 and HCV-229 E has also been reported (12).…”

Section: Diseussionmentioning

confidence: 99%

Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other species

Pedersen

Ward²,

Mengeling

1978

Archives of Virology

136

116

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Utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (FIPV) to 7 other human and animal coronaviruses was studied. FIPV was found to be closely related to transmissible gastroenteritis virus (TGEV) of swine. Transmissible gastroenteritis virus and FIPV were in turn antigenically related to human coronavirus 229E (HCV-229E) and canine coronavirus (CCV). An interesting finding in the study was that the 8 coronaviruses selected for this study fell into one of two antigenically distinct groups. Viruses in each group were antigenically related to each other to varying degrees, but were antigenically unrelated to coronaviruses of the second group. The first antigenically related group was comprised of mouse hepatitis virus, type 3 (MHV-3), hemeagglutinating encephalomyelitis virus 67N (HEV-67N) of swine, calf diarrhea coronavirus (CDCV), and human coronavirus 0C43 (HCV-OC43). The second antigenically related group was comprised of FIPV, TGEV, HCV-229E and CCV.

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“…Coronaviruses were isolated from 18 % of the specimens, but an additional 13 %, which were negative in the laboratory, produced colds when given to volunteers [ 108 ]. Based on these results, which came from a limited age group, it has been estimated that coronaviruses could be responsible for at least 14 % of all respiratory illnesses in a general population [ 109 ].…”

Section: Incidence and Prevalence Of Respiratory Coronavirusesmentioning

confidence: 99%