Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11 1

Chowdhury, E H; Kuribara, Hideo; Hino, Akihiro; Sultana, Parvin; Mikami, Osamu; Shimada, Nobuaki; Guruge, Keerthi S.; Saitô, Masanori; Nakajima, Yasuyuki

doi:10.2527/2003.81102546x

Cited by 96 publications

(94 citation statements)

References 15 publications

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“…Although few have found recombinant DNA sequences in any organ or tissue sample from animals fed GM plants (EFSA, 2007), our results showed that small DNA fragments can also be detected in kids organs when mothers are fed GM soybean. Recombinant plant DNA has also been found by other authors as a rare event (Chowdhury et al, 2003a;Mazza et al, 2005;Sharma et al, 2006) in organs and tissues of animals fed GM feeds. On the contrary, studies aiming at verifying the presence of transgenic fragments in milk gave negative results.…”

Section: R E T R a C T E Dmentioning

confidence: 61%

Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

Tudisco

Mastellone

Cutrignelli

et al. 2010

Animal

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The presence of DNA fragments in blood and milk from goats fed conventional (control) or Roundup Ready R soybean meal solvent extracted (s.e.; treated) was investigated by using a polymerase chain reaction approach. The same investigation was carried out on blood, skeletal muscle and organs from kids of both groups fed only dams' milk until weaning. Moreover, the possible effects on cell metabolism were evaluated by determination of several specific enzymes in serum, heart, skeletal muscle, liver and kidney. Fragments of the multicopy chloroplast (trnL) gene were found in blood and milk samples from goats of both groups. In kids, the chloroplast fragments were found in samples of both groups. In samples, which proved positive for the presence of chloroplast DNA, fragments of the specific soybean single copy gene (lectin) were detected in several blood and milk samples. The same fragment was also found in control and treated groups of kids. Transgenic fragments were not found in those samples, which were found positive for chloroplast fragments of control groups of either goats or kids. On the contrary, in blood and milk of treated goats, fragments both of the 35S promoter and the CP4 EPSPS gene were detected. These fragments were also found in treated kids with a significant detection of the 35S promoter in liver, kidney and blood, and of the CP4 EPSPS gene fragment in liver, kidney, heart and muscle. A significant increase in lactic dehydrogenase, mainly concerning the lactic dehydrogenase-1 isoenzyme was found in heart, skeletal muscle and kidney of treated kids, thus suggesting a change in the local production of the enzyme. Finally, no significant differences were detected concerning kid body and organ weight.

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Section: R E T R a C T E Dmentioning

confidence: 61%

Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

Tudisco

Mastellone

Cutrignelli

et al. 2010

Animal

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“…Several studies have found no detrimental effects of feeding GM maize on the growth of pigs (Reuter et al, 2002;Chowdhury et al, 2003;Custodio et al, 2006;Flachowsky et al, 2007), poultry (Brake et al, 2003;Aeschbacher et al, 2005) or ruminants (Aumaitre et al, 2002;Trabalza-Marinucci et al, 2008). Higher feed intake and a tendency for higher kidney weight were observed in weanling pigs after 30 days of feeding GM maize (Walsh et al, 2011).…”

Section: Introductionmentioning

confidence: 98%

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Buzoianu

Walsh

Rea

et al. 2012

animal

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A total of 72 male weaned pigs were used in a 110-day study to investigate the effect of feeding genetically modified (GM) Bt MON810 maize on selected growth and health indicators. It was hypothesised that in pigs fed Bt maize, growth and health are not impacted compared with pigs fed isogenic maize-based diets. Following a 12-day basal period, pigs (10.7 ± 1.9 kg body weight (BW); ∼40 days old) were blocked by weight and ancestry and randomly assigned to treatments: (1) non-GM maize diet for 110 days (non-GM), (2) GM maize diet for 110 days (GM), (3) non-GM maize diet for 30 days followed by GM maize diet up to day 110 (non-GM/GM) and (4) GM maize diet for 30 days followed by non-GM maize diet up to day 110 (GM/non-GM). BW and daily feed intake were recorded on days 0, 30, 60 and 110 (n= 15). Body composition was determined by dual energy X-ray absorptiometry (n= 10) on day 80. Following slaughter on day 110, organs and intestines were weighed and sampled for histological analysis and urine was collected for biochemical analysis (n= 10). Serum biochemistry analysis was performed on days 0, 30, 60, 100 and 110. Growth performance and serum biochemistry were analysed as repeated measures with time and treatment as main factors. Thesliceoption of SAS was used to determine treatment differences at individual time points. There was no effect of feeding GM maize on overall growth, body composition, organ and intestinal weight and histology or serum biochemistry on days 60 and 100 and on urine biochemistry on day 110. A treatment × time interaction was observed for serum urea (SU;P< 0.05), creatinine (SC;P< 0.05) and aspartate aminotransferase (AST;P< 0.05). On day 30, SU was lower for the non-GM/GM treatment compared with the non-GM, GM and GM/non-GM treatments (P< 0.05). On day 110, SC was higher for the non-GM/GM and GM/non-GM treatments compared with non-GM and GM treatments (P< 0.05). Overall, serum total protein was lower for the GM/non-GM treatment compared with the non-GM/GM treatment (P< 0.05). The magnitude of change observed in some serum biochemical parameters did not indicate organ dysfunction and the changes were not accompanied by histological lesions. Long-term feeding of GM maize to pigs did not adversely affect growth or the selected health indicators investigated.

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“…None were detected in the liver, spleen, or lymph nodes indicating that they were too large to be systematically absorbed from the GI tract (Chowdhury et al 2003). It was suggested that transgenic nucleic acid and proteins from GM crops are handled in the gut like their conventional counterparts, with no evidence for systemic absorption of intact proteins or genes (Sieradzki et al 2013).…”

Section: World Controversymentioning

confidence: 99%

Genetically engineered (modified) crops (Bacillus thuringiensis crops) and the world controversy on their safety

Abbas¹

2018

Egypt J Biol Pest Control

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Bacillus thuringiensis (Bt) crops are plants genetically engineered (modified) to contain the endospore (or crystal) toxins of the bacterium, Bt to be resistant to certain insect pests. In 1995, the Environmental Protection Agency (EPA) in USA approved the commercial production and distribution of the Bt crops: corn, cotton, potato, and tobacco. Currently, the most common Bt crops are corn and cotton. The crystal, referred to as Cry toxins, is proteins formed during sporulation of some Bt strains and aggregate to form crystals. Such Cry toxins are toxic to specific species of insects belongs to orders: Lepidoptera, Coleoptera, Hymenoptera, Diptera, and Nematoda. In 2016, the total world area cultivated with genetically modified crops (GM crops) reached about 185 million ha. This review shows that there is a worldwide controversy about the safety of Bt crops to the environment and mammals. Some researchers support the cultivation of Bt crops depending upon the results of their laboratory and field studies on the safety of such crops. Others, however, are against Bt crops as they may cause risk to human.

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Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11 1

Cited by 96 publications

References 15 publications

Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Genetically engineered (modified) crops (Bacillus thuringiensis crops) and the world controversy on their safety

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