Detection of CNV in the SH3RF2 gene and its effects on growth and carcass traits in chickens

Abstract

Background: The SH3RF2 gene is a protein-coding gene located in a quantitative trait locus associated with body weight, and its deletion has been shown to be positively associated with body weight in chickens.Results: In the present study, CNV in the SH3RF2 gene was detected in 4079 individuals from 17 populations, including the "Gushi ×Anka" F2 resource population and populations of Chinese native chickens, commercial layers, and commercial broilers. The F2 resource population was then used to investigate the genetic effects of the chicken SH3RF2 gene. The results showed that the local chickens and commercial layers were all homozygous for the wild-type allele. Deletion mutation individuals were detected in all of the commercial broiler breeds except Hubbard broiler. A total of, 798 individuals in the F2 resource group were used to analyze the effects of genotype (DD/ID/II) on chicken production traits. The results showed that CNV was associated with 2-, 6-, 10-, and 12-week body weight (P = 0.026, 0.042, 0.021 and 0.039 respectively) and significantly associated with 8-week breast bone length (P = 0.045). The mutation was significantly associated with 8-week body weight (P = 0.007) and 4-week breast bone length (P = 0.010). CNV was significantly associated with evisceration weight, leg muscle weight, carcass weight, breast muscle weight and gizzard weight (P = 0.032, 0.033, 0.045, 0.004 and 0.000, respectively). Conclusions: CNV of the SH3RF2 gene contributed to variation in the growth and weight gain of chickens.

Comprehensive analysis of structural variants in chickens using PacBio sequencing

“…There is still work to be done on the genetic mechanisms of rumplessness in Piao chickens. Our structural variation results also revealed some candidate genes that have been reported to be associated with chicken feather color ( SOX10, CDKN2A ) ( Gunnarsson et al, 2011 ; Schwochow Thalmann et al, 2017 ), growth ( MYF5, IGF2BP1, CRY1; SH3RF2 ) ( Jing et al, 2020 ; Niessner et al, 2011 ; Krause et al, 2016 ; Zhang et al, 2020 ; Wang et al, 2021 ).…”

The Chicken Pan-Genome Reveals Gene Content Variation and a Promoter Region Deletion in IGF2BP1 Affecting Body Size

“…S3 c , Supplementary Material online). The well-studied SH3 domain containing ring finger 2 ( SH3RF2 , ENSGAT00000090177 ) gene, regulating appetite and affecting body weight, was also identified as one of these genes that is completely absent in HWS but present in LWS ( Rubin et al 2010 ; Jing et al 2020 ).…”

Variability of Meat and Carcass Quality from Worldwide Native Chicken Breeds

“…Some poultry breeds have been exposed to high selective pressure in terms of body size. Indeed, quantitative trait locus, with main effects on the shank length, growth, weight gain, and body and carcass weights have been mapped a priori, which may be indicative of the relevance that these traits have [ 92 , 153 ].…”