Detection of CMY-2, CTX-M-14, and SHV-12 β-Lactamases in
            <i>Escherichia coli</i>
            Fecal-Sample Isolates from Healthy Chickens

Briñas, Laura; Moreno, Miguel A.; Zarazaga, Myriam; Porrero, Concepción; Sáenz, Yolanda; García, María L.; Domı́nguez, Lucas; Torres, Carmén

doi:10.1128/aac.47.6.2056-2058.2003

Cited by 176 publications

(123 citation statements)

References 26 publications

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“…This observation is in good agreement with the previous publication of Bradford et al (10) reporting the location of genes encoding CTX-M-5 on small non-self-transferable plasmids. It may also explain the relatively low proportion of CTX-M-5-producing isolates compared to those bearing CTX-M-3 and Neither CTX-M-8-nor CTX-M-9-related enzymes were found in this study, although in recent years ESBLs of the latter genetic group were found in other European countries (Spain [9,11,35], France [18,36], and the United Kingdom [Alobwede et al, letter]) as well as in Asian countries (Japan [41], China [14], Taiwan [22,44], Korea [28], and Vietnam [13]) and Brazil (8).…”

Section: Resultscontrasting

confidence: 44%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

327

181

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A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum ␤-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for CTX-M-type ␤-lactamases, which, as shown by PCR-restriction fragment length polymorphism analysis, were distributed into the two genetic groups of CTX-M-1 (93%)-and CTX-M-2 (7%)-related enzymes. Isolates producing the enzymes of the first group were found in 20 hospitals from geographically distant regions of the country and were characterized by considerable diversity of genetic types, as was demonstrated by enterobacterial repetitive consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15 ␤-lactamases were identified. In contrast, the enzymes of the CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally related E. coli isolates from a single hospital. Notably, the levels of resistance to ceftazidime were remarkably variable among the CTX-M producers. This study provides further evidence of the global dissemination of CTX-M type ESBLs and emphasizes the need for their epidemiological monitoring.During the past decade extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type emerged in many countries of the world. The first organisms producing ␤-lactamases of this type were identified both as single and epidemic clinical isolates in very distant geographic regions (Germany and France and Argentina) in the early 1990s (3, 5, 7). More recently, a rapid increase in the proportion of multiple CTX-M variants to the TEM-and SHV-derived ESBLs has been reported in many hospitals in Spain (9,12,15,35) (14), and Korea (28). Furthermore, CTX-M ␤-lactamases, mainly types CTX-M-2 and CTX-M-3, were found to be widespread or even predominant ESBL types in several countries, including Argentina (31, 34; M. Galas, F. Pasteran, R. Melano, A. Petroni, G. Lopez, A. Corso, A. Rossi, et al., Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-109, 1998), Japan (41), and Poland (1).Currently the CTX-M family includes more than 20 ␤-lactamases, which may be grouped on the basis of sequence similarity into four distinct clusters (subtypes) epitomized by CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 (26, 37; http: //www.lahey.org/studies/webt.htm). Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of ...

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Section: Resultscontrasting

confidence: 44%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

327

181

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“…β-lactamase and ESBL's have also been detected in E. coli isolates from healthy chickens, food and sick animals in Spain (Brinas et al, 2003a;Brinas et al, 2003b;Brinas et al, 2002). The use of extended-spectrum cephalosporins in chickens is very unusual, and the possibility of cross-selection with other antimicrobials used in poultry (such as sulphonamides and tetracyclines, among others) might explain this discovery.…”

Section: E Coli Strains Producing An Extended Spectrum β-Lactamase (mentioning

confidence: 95%

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Gaze

O’Neill

Wellington

et al. 2008

Advances in Applied Microbiology

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“…The first ESBL-producing E. coli (CTX-M-14, SHV-12, and CMY-2) in poultry were isolated from healthy chick feces in Spain in 2000 and 2001 (7). Recently published literature reported that the most common ESBL-type E. coli in healthy poultry were CTX-M-1 in France (12) CTX-M-65) and TEM in China (14).…”

Section: Discussionmentioning

confidence: 99%

“…Randomly choosing a colony from each cloacal sample, bla CTX-M (CTX-M groups 1, 8, 9, and 10), bla TEM, bla OXA-1, bla FOX, bla CMY, bla SHV , and ampC beta lactamase genes were studied using PCR (7,8). All obtained amplicons were sequenced on both strands, and sequences were compared with those included in the GenBank database and on the website http:/www.lahey.org/Studies/ to identify the betalactamase genes.…”

Section: Characterization Of Esbl Genesmentioning

confidence: 99%

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

Ünal¹,

Karagöz²,

Aşkar³

et al. 2017

Turk J Vet Anim Sci

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IntroductionEscherichia coli strains are important pathogens in animals and humans, and are normal commensals in intestinal tracts. The β-lactam group of antibiotics has been intensely used in human and veterinary medicine as infectious disease treatments (1). Extended-spectrum betalactamases (ESBLs) hydrolyze oxyimino-cephalosporins and give resistance to bacteria against the penicillins, cephalosporins (first, second, and third generations), and aztreonam. They are repressed by β-lactamase inhibitors. ESBLs are classified as β-lactamases containing three main families: TEM (named after the patient Temoneria), SHV (sulfhydryl reagent variable), and CTX-M (active on cefotaxime, first isolated in Munich) (2). The prevalence of CTX-M-type β-lactamase-producing E. coli has increased in poultry over the last decade (3).Increasing prevalence of ESBL-producing E. coli in poultry has become a major zoonotic risk because of possible transmission from broilers to humans (3). In addition, ESBL-producing E. coli strains that may have multidrug resistance (MDR) and cause therapeutic problems have been reported in human and veterinary practice (4). The alarming spread of ESBL-producing Enterobacteriaceae poses a serious public health threat and has attracted the attention of scientists, politicians, and the general public globally. There was no previous study about the presence, prevalence, and molecular epidemiology of ESBL-producing E. coli isolates from broilers in Turkey. The aim of the present study was to investigate the resistance phenotype and genotyping characteristics of ESBL-carrying E. coli strains in healthy broilers in Turkey. Materials and methods Samples, isolations, and identificationFrom January to April 2012, 300 samples were collected from cloacal samples of healthy slaughterhouse broilers in two regions of Turkey. Broiler cloacal samples were obtained from a total of 30 different poultry houses

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Detection of CMY-2, CTX-M-14, and SHV-12 β-Lactamases in Escherichia coli Fecal-Sample Isolates from Healthy Chickens

Cited by 176 publications

References 26 publications

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Antibiotic Resistance in the Environment, with Particular Reference to MRSA

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

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