Detection of CMV in urine: comparison between DNA-DNA hybridization, virus isolation, and immunoelectron microscopy

Vonsover, Ami; Gotlieb-Stematsky, T.; Sayar, Y; Bardov, L.; Manor, Yossi; Siegal, B

doi:10.1016/0166-0934(87)90028-0

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Diagnostic Procedures1

Purification O F Viral Particles1

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1988

2024

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Cited by 8 publications

(2 citation statements)

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“…The dot blot hybridization has been demonstrated to be a reliable method for testing clinical specimens for the presence of H CMV (Chou & Merigan, 1983 ;Schuster et al, 1986;Virtanen et al, 1984;Vonsover et al, 1987). Southern blot hybridizations are more sensitive and allow more detailed studies on HCMV infection (Adler, 1985;Rfiger & Fleckenstein, 1985;Spector et al, 1985).…”

Section: Diagnostic Proceduresmentioning

confidence: 99%

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

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Section: Diagnostic Proceduresmentioning

confidence: 99%

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

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“…Antibodies were detected by clumping test [15] and by solidphase immune electron microscopy (SPIEM), as described by Vonsover et al [16]. Both methods involve incubation of antibodies with the virus and visualization of the resulting agglutination (clumping) of virions by EM.…”

Section: Purification O F Viral Particlesmentioning

confidence: 99%

Aplastic Crisis Due to Human B19 Parvovirus Infection in Red Cell Pyrimidine-5′ -Nucleotidase Deficiency

et al. 1989

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Two siblings with chronic hemolytic anemia due to red cell pyrimidine-5′ -nucleotidase (P-5′-N) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. The cause of the aplastic crisis was an acute infection with human B19 parvovirus (B19 HPV) as proven by immunoelectron microscopy and DNA hybridization. This is the first report on the association of B19-HPV-related aplastic crisis with P-5′-N deficiency.

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