Detection of Clostridium septicum Hemolysin Gene by Polymerase Chain Reaction.

Takeuchi, Shinichi; Hashizume, Naoko; Kinoshita, Takafumi; Kaidoh, Toshiö; Tsutsumi, Yutaka

doi:10.1292/jvms.59.853

Cited by 35 publications

(14 citation statements)

References 20 publications

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“…Bacterial cells in late exponential phase (1 ml culture) were centrifuged and resuspended in 150 µl sucrose\TES (25 %, w\v, sucrose, 10 mM Tris\HCl, 1 mM EDTA, 150 mM NaCl) containing 4 mg lysozyme ml −" . DNA extraction and purification were carried out according to Takeuchi et al (1997). For PCR, primers 16SUNI-L and UNI16S-R (Kuhnert et al, 1996) were used to amplify a 1500 bp region of the 16S rDNA sequences.…”

Section: Abstract : Clostridium Novyi Types a B And C Clostridium Hmentioning

confidence: 99%

Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences.

Sasaki¹,

Takikawa²,

Kojima³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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The partial sequences (1465 bp) of the 16S rDNA of Clostridium novyi types A, B and C and Clostridium haemolyticum were determined. C. novyi types A, B and C and C. haemolyticum clustered with Clostridium botulinum types C and D. Moreover, the 16S rDNA sequences of C. novyi type B strains and C. haemolyticum strains were completely identical ; they differed by 1 bp (level of similarity S 999 %) from that of C. novyi type C, they were 987 % homologous to that of C. novyi type A (relative positions 28-1520 of the Escherichia coli 16S rDNA sequence) and they exhibited a higher similarity to the 16S rDNA sequence of C. botulinum types D and C than to that of C. novyi type A. These results suggest that C. novyi types B and C and C. haemolyticum may be one independent species generated from the same phylogenetic origin.

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Section: Abstract : Clostridium Novyi Types a B And C Clostridium Hmentioning

confidence: 99%

Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences.

Sasaki¹,

Takikawa²,

Kojima³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…During the last 20 years there has been intensive development of technologies to identify microbial species and to estimate their abundancies using polymerase chain reaction (PCR; Morandi, Cremonesi, Silvetti, Castiglioni, & Brasca, 2015;Schmidt, Emara, & Kung, 2008;Takeuchi, Hashizume, Kinoshita, Kaidoh, & Tamura, 1997). Currently, a major part of silage studies utilizing PCR-based methods have focused on the effects of microbial inoculants in ensiling (Klocke et al, 2006;Muck, 2013;Schmidt & Kung, 2010).…”

Section: Introductionmentioning

confidence: 99%

The effect of additives on the quality of white lupin–wheat silage assessed by fermentation pattern and qPCR quantification of clostridia

König

Lamminen

Weiß

et al. 2017

Grass and Forage Science

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The efficacy of different silage additives on different mixtures of white lupin and spring wheat was investigated in four separate trials. The bicrop was harvested 96 days (trials 1 and 2) and 110 days (trials 3 and 4) after sowing. For each maturity stage, two mixtures of white lupin and spring wheat were reformed in the ratios of 1:2 and 2:1 on fresh matter (FM) basis respectively. The crops were treated with formic acid (FA), sodium nitrite–hexamine mixture (NaHe) or homofermentative lactic acid bacteria (LAB). The control silage was made without additive. Additives were not able to improve the quality of white lupin–wheat silage in all trials, compared with untreated silage. The treatment with LAB showed good results only at the first stage of crop maturity with sufficient amounts of water‐soluble carbohydrate in the pre‐ensiling crops. The FA treatment showed elevated butyric acid levels in all trials, which suggests that the FA application level used (4 L t−1 FM, 100% FA) was insufficient to decrease pH enough for preventing the growth of clostridia and butyric acid fermentation. NaHe was the only additive that was able to inhibit the activity of clostridia in all trials.

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“…However, both species are considered not to be involved in malignant edema. As an alternative, primer pairs and a probe targeting the hemolysin gene of C. septicum could be included in the assay (17).…”

Section: Discussionmentioning

confidence: 99%

Novel Real-Time PCR Assay for Simultaneous Detection and Differentiation of Clostridium chauvoei and Clostridium septicum in Clostridial Myonecrosis

et al. 2010

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A real-time PCR assay based on the 16S rRNA gene sequence was designed for differentiation of blacklegcausing Clostridium chauvoei and Clostridium septicum, a phylogenetically closely related bacterium responsible for malignant edema. In order to exclude false-negative results, an internal amplification control was included in the assay. A set of three probes, one specific for C. chauvoei, one specific for C. septicum, and one specific for both species, permitted unequivocal detection of C. chauvoei in tests of 32 Clostridium sp. strains and 10 non-Clostridium strains. The assay proved to be sensitive, detecting one genome of C. chauvoei or C. septicum per PCR and 1.79 ؋ 10 3 C. chauvoei cells/g artificially contaminated muscle tissue. In tests of 11 clinical specimens, the real-time PCR assay yielded the same results as an established conventional PCR method.

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Detection of Clostridium septicum Hemolysin Gene by Polymerase Chain Reaction.

Cited by 35 publications

References 20 publications

Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences.

Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences.

The effect of additives on the quality of white lupin–wheat silage assessed by fermentation pattern and qPCR quantification of clostridia

Novel Real-Time PCR Assay for Simultaneous Detection and Differentiation of Clostridium chauvoei and Clostridium septicum in Clostridial Myonecrosis

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