Detection of Clostridium perfringens epsilon toxin by elisa

Naylor, R.D.; Martin, P.; Sharpe, R.T.

doi:10.1016/s0034-5288(18)30696-9

Cited by 41 publications

(22 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The toxin has been demonstrated in the intestinal content of some healthy sheep (Bullen 1952, Naylor and others 1987). Bullen and others (1953) demonstrated experimentally that sheep can tolerate low concentrations of epsilon toxin in the intestine without ill effects.…”

Section: Introductionmentioning

confidence: 99%

Clostridium perfringens type‐D enterotoxaemia in cattle: the diagnostic significance of intestinal epsilon toxin

Jones¹,

Dagleish

Caldow³

2015

Veterinary Record

View full text Add to dashboard Cite

The aims of this study were to describe 42 cases of Clostridium perfringens type-D enterotoxaemia in cattle seen between 2003 and 2014 and to determine the diagnostic value of detecting epsilon toxin in bovine intestinal content. All cases in the series had histological brain changes considered pathognomonic for C. perfringens type-D enterotoxaemia in sheep and goats and the epsilon toxin of C. perfringens was concurrently detected in the intestinal contents of 15 (36 per cent) cases. The data from the case series indicate that intestinal epsilon toxin has a sensitivity of 56 per cent compared with histology of the brain for diagnosis of bovine C. perfringens type-D enterotoxaemia. The diagnostic specificity of detecting epsilon toxin in bovine intestinal content was investigated by screening intestinal contents of 60 bovine carcases submitted for postmortem examination. Epsilon toxin was detected in 11 (18 per cent) carcases but no pathognomonic histological brain change was found in any. The specificity of intestinal epsilon toxin was estimated to be 80.4 per cent. These studies demonstrate that for a definitive diagnosis of C. perfringens type-D enterotoxaemia in cattle histological examination of the brain is essential as the presence of epsilon toxin in the intestinal contents alone is neither sensitive nor specific enough.

show abstract

Section: Introductionmentioning

confidence: 99%

Clostridium perfringens type‐D enterotoxaemia in cattle: the diagnostic significance of intestinal epsilon toxin

Jones¹,

Dagleish

Caldow³

2015

Veterinary Record

View full text Add to dashboard Cite

show abstract

“…2004). Different toxin types of C. perfringens strains have also been screened with commercial enzyme‐linked immunosorbent assay kits (Naylor et al. 1987; Nagahama et al.…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Heikinheimo

Korkeala

2005

Lett Appl Microbiol

View full text Add to dashboard Cite

Aims: The aim of the study was to determine the presence of genes coding for alpha (cpa), beta (cpb), epsilon (etx), iota (iA) and enterotoxin (cpe) from Clostridium perfringens broiler chicken isolates, using multiplex PCR assay established in the study. Methods and Results: The multiplex PCR assay was shown to be specific when tested with 10 C. perfringens strains representing different toxin types, and 15 strains of other bacterial species. All 118 broiler chicken C. perfringens isolates were shown to carry the cpa gene but not cpb, etx, iap or cpe genes, signifying that all isolates represented type A and were cpe-negative. Conclusions: The assay established in the study enables the simultaneous detection of the major toxin genes and the cpe gene from C. perfringens isolates. Significance and Impact of the Study: The present study offers a new primer pair for detecting cpa, combined with a multiplex PCR assay. In addition, the study provides data of the presence of different toxin genes in C. perfringens isolates obtained from broiler chickens.

show abstract

“…5,6,12 Different techniques are routinely used to detect epsilon toxin in body fluids. 4,6,8,9,12,16 In the present study the 4 techniques tested showed marked differences in their ability to detect epsilon toxin in intestinal content and other body fluids. The PC-ELISA was the most sensitive technique regardless of fluid type.…”

Section: Discussionmentioning

confidence: 57%

“…The techniques most commonly used to detect epsilon toxin in intestinal contents and other body fluids are the mouse neutralization test (MNT), 12 several enzyme-linked immunosorbent assays (ELISAs), 4,8,9,16 and counterimmunoelectrophoresis (CIEP). 6 There is little information available in the literature about the comparative ability of these methods to detect epsilon toxin in intestinal contents and other body fluids.…”

mentioning

confidence: 99%

Comparison of Four Techniques for the Detection of Clostridium Perfringens Type D Epsilon Toxin in Intestinal Contents and other Body Fluids of Sheep and Goats

et al. 2003

View full text Add to dashboard Cite

Abstract. Polyclonal capture enzyme-linked immunosorbent assay (PC-ELISA), monoclonal capture ELISA (MC-ELISA), mouse neutralization test (MNT), and counterimmunoelectrophoresis (CIEP), were compared for their ability to detect epsilon toxin in intestinal contents and body fluids of sheep and goats. When used to evaluate intestinal contents of sheep artificially spiked with epsilon prototoxin, PC-ELISA detected 0.075 mouse lethal dose (MLD) 50 /ml, whereas the MNT, MC-ELISA, and CIEP detected 6, 25, and 50 MLD 50 /ml, respectively. Amounts of epsilon toxin detected by PC-ELISA, MC-ELISA, MNT, and CIEP in sheep pericardial fluid artificially spiked with epsilon prototoxin were 0.075, 0.75, 6, and 200 MLD 50 /ml, respectively. For assaying epsilon toxin in aqueous humor, PC-ELISA and MC-ELISA detected 0.075 MLD 50 / ml, whereas CIEP detected 200 MLD 50 /ml (MNT was not evaluated). When 51 samples of intestinal contents of sheep and goats (32 positive and 19 negative to MNT) were analyzed by the other 3 techniques, the relative sensitivity of PC-ELISA, MC-ELISA, and CIEP was 93.75, 84.37, and 37.50%, respectively. The specificity of PC-ELISA, MC-ELISA, and CIEP was 31.57, 57.89, and 84.21%, respectively. The absolute sensitivity of PC-ELISA, MC-ELISA, CIEP, and MNT was 90.90, 69.69, 15.15, and 54.54%. The absolute specificity of the 4 techniques was 100%. These results show that there is a marked inconsistency among techniques routinely used to detect Clostridium perfringens epsilon toxin. Until more consistent results are achieved, the diagnosis of enterotoxemia should not only be based solely on epsilon toxin detection, but also on clinical and pathological data.

show abstract

Detection of Clostridium perfringens epsilon toxin by elisa

Cited by 41 publications

References 7 publications

Clostridium perfringens type‐D enterotoxaemia in cattle: the diagnostic significance of intestinal epsilon toxin

Clostridium perfringens type‐D enterotoxaemia in cattle: the diagnostic significance of intestinal epsilon toxin

Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Comparison of Four Techniques for the Detection of Clostridium Perfringens Type D Epsilon Toxin in Intestinal Contents and other Body Fluids of Sheep and Goats

Contact Info

Product

Resources

About