Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan)

Volkmann, Holger; Schwartz, Thomas; Bischoff, P.; Kirchen, Silke; Obst, Ursula

doi:10.1016/j.mimet.2003.10.014

Cited by 209 publications

(135 citation statements)

References 31 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…The greatest anxiety is that their presence will lead to antibacterial resistance in biological wastewater (WW) treatment and the environment (Andersson 2003;Costanzo et al 2005;Daughton 2003;Daughton and Ternes 1999;Goni-Urriza et al 2000;Halling-Sorensen et al 1998;Kümmerer 2009b;Reinthaler et al 2003;Volkmann et al 2004). Recently, this has been demonstrated by the observed increase in antibacterial resistance in Acinetobacter spp.…”

Section: Smx Usage and Presence In The Environmentmentioning

confidence: 99%

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Larcher

Yargeau

2012

Appl Microbiol Biotechnol

119

View full text Add to dashboard Cite

Antibiotic compounds, like sulfamethoxazole (SMX), have become a concern in the aquatic environment due to the potential development of antibacterial resistances. Due to extensive consumption, excretion and disposal, SMX has been frequently detected in wastewaters and surface waters. This has led to numerous studies investigating the nature of SMX, with many researchers focusing on the biodegradation and persistence of SMX during wastewater treatment and in the environment. This review provides a summary of recent developments, outlines the discrepancies in observations and results, and demonstrates the need for further research to determine optimal biological removal strategies for SMX and other antibiotics.

show abstract

Section: Smx Usage and Presence In The Environmentmentioning

confidence: 99%

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Larcher

Yargeau

2012

Appl Microbiol Biotechnol

119

View full text Add to dashboard Cite

show abstract

“…Antibiotic compounds represent one of the largest concerns as it is believed that their presence will lead to antibacterial resistances in bacteria present in the environment and in biological wastewater treatment (activated sludge) (Andersson 2003;Costanzo et al 2005;Daughton 2003;Daughton and Ternes 1999;Goni-Urriza et al 2000;Jorgensen and Halling-Sorensen 2000;Reinthaler et al 2003;Volkmann et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of sulfamethoxazole by individual and mixed bacteria

Larcher

Yargeau

2011

Appl Microbiol Biotechnol

148

View full text Add to dashboard Cite

Antibiotic compounds, like sulfamethoxazole (SMX), have become a concern in the aquatic environment due to the potential development of antibacterial resistances. Due to excretion and disposal, SMX has been frequently detected in wastewaters and surface waters. SMX removal in conventional wastewater treatment plants (WWTPs) ranges from 0% to 90%, and there are opposing results regarding its biodegradability at lab scale. The objective of this research was to determine the ability of pure cultures of individual and mixed consortia of bacteria (Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas putida, Rhodococcus equi, Rhodococcus erythropolis, Rhodococcus rhodocrous, and Rhodococcus zopfii) known to exist in WWTP activated sludge to remove SMX. Results showed that R. equi alone had the greatest ability to remove SMX leading to 29% removal (with glucose) and the formation of a metabolite. Degradation pathways and metabolite structures have been proposed based on the potential enzymes produced by R. equi. When R. equi was mixed with other microorganisms, a positive synergistic effect was not observed and the maximum SMX removal achieved was 5%. This indicates that pure culture results cannot be extrapolated to mixed culture conditions, and the methodology developed here to study the biodegradability of compounds under controlled mixed culture conditions offers an alternative to conventional studies using pure bacterial cultures or inocula from activated sludge sources consisting of unknown and variable microbial populations.

show abstract

“…Previous studies have shown that S. aureus has a low prevalence in hospital and municipal wastewaters ( [Schwartz et al, 2003] and [Shannon et al, 2007]). The mecA gene has been detected both in municipal and in hospital wastewaters (Börjesson et al, in press;[Schwartz et al, 2003] and [Volkmann et al, 2004]). …”

Section: Introductionmentioning

confidence: 99%

A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant

et al. 2009

View full text Add to dashboard Cite

The spread of methicillin-resistant Staphylococcus aureus (MRSA), in which the mecA gene mediates resistance, threatens the treatment of staphylococcal diseases. The aims were to determine the effect of wastewater treatment processes on mecA gene concentrations, and the prevalence of S. aureus and MRSA over time. To achieve this a municipal wastewater treatment plant was investigated for the mecA gene, S. aureus and MRSA, using real-time PCR assays. Water samples were collected monthly for one year, at eight sites in the plant, reflecting different aspects of the treatment process. The mecA gene and S. aureus could be detected throughout the year at all sampling sites. MRSA could also be detected, but mainly in the early treatment steps. The presence of MRSA was verified through cultivation from inlet water. The concentration of the mecA gene varied between months and sampling sites, but no obvious seasonal variation could be determined. The wastewater treatment process reduced the mecA gene concentration in most months. Taken together our results show that the mecA gene, S. aureus and MRSA occur over the year at all sites investigated.

show abstract

Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan)

Cited by 209 publications

References 31 publications

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Biodegradation of sulfamethoxazole by individual and mixed bacteria

A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant

Contact Info

Product

Resources

About