Detection of Clavibacter michiganensis subsp. sepedonicus in daughter tubers of volunteer potato plants

Abstract

Pánková I., Krejzar V., Čepl J., Kůdela V. (2007): Detection of Clavibacter michiganensis subsp. sepedonicus in daughter tubers of volunteer potato plants. Plant Protect. Sci., 43: 127-134.Daughter tubers of volunteer potatoes were tested for their ability to maintain Clavibacter michiganensis subsp. sepedonicus (Cms). In different areas of the CR, volunteer potatoes were searched for in crops grown in rotation with potatoes and where one or two years before Cms had been detected and identified in samples of harvested seed or commercial potatoes using the test scheme in accordance to EC Directive 93/85/EEC. During May and June of 2005 and 2006, emerging or emerged plants of volunteer potatoes were collected at nine locations of Bohemia and transplanted to the experimental field in the Diagnostic Service Laboratory at Šluknov-Kunratice in Northern Bohemia. The daughter tubers of these plants were harvested and stored at 6°C for 1 month and then at 22°C for 3 months for multiplication of Cms cells. Samples of the daughter tubers were divided into 215 partial samples and tested for the occurrence of Cms at five terms which differed in length of storage time. The DAS ELISA test was used to detect Cms in the tuber samples. Cms was detected in eight of the nine potato volunteer tuber samples from different locations. The presence of Cms in positively tested tuber samples was confirmed using a pathogenicity test on eggplants (Solanum melongena). The optimal time for the detection of the pathogen in the harvested daughter tubers was between 4 and 10 weeks of storage at 22°C.

Bacterial ring rot of potato caused by Clavibacter sepedonicus: A successful example of defeating the enemy under international regulations

“…The bacterium can persist in a field on the surface or inside unharvested potato tubers. Daughter tubers of volunteer potato plants growing within a nonpotato crop cultivated in rotation with potatoes could also act as reservoirs of the pathogen in the field (Pánková et al, 2007). While other species of Clavibacter can grow in a variety of environmental and plant‐associated niches, C .…”

“…Daughter tubers of volunteer potato plants growing within a nonpotato crop cultivated in rotation with potatoes could also act as reservoirs of the pathogen in the field (Pánková et al, 2007). While other species of Clavibacter can grow in a variety of environmental and plant-associated niches, C. sepedonicus is almost entirely restricted to the vascular system of its host plant.…”

“…Semicommercial electronic devices are available to detect ring rot of potato by recognizing volatile compounds emitted by the infected potato tubers (Biondi et al, 2014). The optimal time for the detection of the pathogen in harvested daughter tubers is after 4–10 weeks of storage at 22°C (Pánková et al, 2007), while the recommended standard sample size is at least 200 tubers per 25 tonnes (EFSA et al, 2019). If an infection is confirmed, an extensive trace‐back of the origin and a trace‐forward of possible spread should be performed.…”

Reaction of selected potato varieties to Clavibacter sepedonicus infestation under changing weather conditions

“…The occurrence of symptoms of Cs infection on potato leaves and in potato tubers is dependent on many pathogen-plant interactions. The size of the pathogen population in the plant causing the infection and the occurrence of symptoms varies significantly between potato varieties (Bishop and Slack 1987a, De Boer and McCann 1990, Kawchuk et al 1998, Pietraszko et al 2018, Pánková et al 2007, Pánková et al 2019, Whitworth et al 2019. Hill et al 2011 believe that the degree of infection and the rate of Cs population development in the plant are more influenced by the habitat conditions such as temperature and humidity rather than varietal characteristics.…”