Detection of class I MHC mRNA in subpopulations of first trimester cytotrophoblast cells by in situ hybridization

Hunt, Joan S.; Hsi, Bae‐Li; King, Charles R.; Fishback, James L.

doi:10.1016/0165-0378(91)90043-p

Cited by 28 publications

(20 citation statements)

References 21 publications

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“…At present, the cause for the differential transcription of MEST/Mest is unknown. In the human trophoblast, expression even of functionally important genes like that coding for HLA-G are regulated on the translational level (Hunt et al, 1991). Thus, it remains an important question, whether the differential MEST/Mest transcription pattern present in mouse and human trophoblast is reflected also at the protein level.…”

Section: Discussionmentioning

confidence: 99%

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

et al. 2000

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Section: Discussionmentioning

confidence: 99%

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

et al. 2000

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“…In contrast to APC, fibroblasts and cells of endothelial and epithelial origin do not express class II Ags constitutively, but exposure to IFN-␥ induces class II expression (2,10). Finally, trophoblasts and sensory neurons do not express class II Ags, either constitutively or after exposure to cytokines (11)(12)(13).…”

mentioning

confidence: 99%

DNA Alkylating Agents Alleviate Silencing of Class II Transactivator Gene Expression in L1210 Lymphoma Cells

Murphy

Holtz

Lewandowski

et al. 2002

The Journal of Immunology

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MHC class II (Ia) Ag expression is inversely correlated with tumorigenicity and directly correlated with immunogenicity in clones of the mouse L1210 lymphoma (1 ). Understanding the mechanisms by which class II Ag expression is regulated in L1210 lymphoma may facilitate the development of immunotherapeutic approaches for the treatment of some types of lymphoma and leukemia. This study demonstrates that the variation in MHC class II Ag expression among clones of L1210 lymphoma is due to differences in the expression of the class II transactivator (CIITA). Analysis of stable hybrids suggests that CIITA expression is repressed by a dominant mechanism in class II-negative L1210 clones. DNA-alkylating agents such as ethyl methanesulfonate and the chemotherapeutic drug melphalan activate CIITA and class II expression in class II negative L1210 cells, and this effect appears to be restricted to transformed cell lines derived from the early stages of B cell ontogeny. Transient transfection assays demonstrated that the CIITA type III promoter is active in class II− L1210 cells, despite the fact that the endogenous gene is not expressed, which suggests that these cells have all of the transacting factors necessary for CIITA transcription. An inverse correlation between methylation of the CIITA transcriptional regulatory region and CIITA expression was observed among L1210 clones. Furthermore, 5-azacytidine treatment activated CIITA expression in class II-negative L1210 cells. Collectively, our results suggest that 1) CIITA gene expression is repressed in class II− L1210 cells by methylation of the CIITA upstream regulatory region, and 2) treatment with DNA-alkylating agents overcomes methylation-based silencing of the CIITA gene in L1210 cells.

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“…The anti-human leukocyte antigen (HLA)-G has been reported to be a good marker of EVTs within the decidua (Hunt et al, 1991;Kovats et al, 1990). However, the HLA-G antibody used in the present study is specific to the soluble form of HLA-G (sHLA-G).…”

Section: Dunk Et Almentioning

confidence: 44%

A Novel In Vitro Model of Trophoblast-Mediated Decidual Blood Vessel Remodeling

Dunk

Petković

Baczyk

et al. 2003

Laboratory Investigation

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SUMMARY:In vivo the extravillous trophoblasts (EVTs) penetrate the decidua and the first third of the myometrium to remodel the uterine spiral arteries and achieve the high-flow, low-resistance circulation characteristic of the intervillous space of the term placenta. Much of our understanding of these processes comes from histologic analysis of placental bed biopsies, a limited tissue source and one that can provide only a snapshot of a dynamic process. To better characterize these cellular interactions, we have developed an in vitro co-culture system in which first trimester villous explants are cultured at low oxygen tension in contact with 2-mm 2 sections of decidua parietalis from the same patient. Hematoxylin eosin counterstaining of paraffin sections shows that EVT columns form at the tips of the placental villi and adhere and penetrate the decidual surface. The decidual blood vessels in the path of the EVT show morphologic disruption. Immunohistochemical analysis of the co-cultures using both an endothelial specific anti-CD31 and an anti-smooth muscle actin antibody show a disruption of the integrity of the vessel lining together with a complete loss of organized smooth muscle actin surrounding the blood vessels. In contrast control decidua samples in the absence of placental villi exhibit blood vessels with a complete endothelial lining and an organized muscular sheath. Using both an anti-cytokeratin-7 and anti-Cdx-2 antibody specific to trophoblasts, we show that these changes coincide with invasion of the vessels by endovascular trophoblasts and penetration of the decidua by interstitial EVTs. No EVTs were found in the control decidua. Thus we conclude that this in vitro model mimics the physiologic change observed in vivo during trophoblast invasion into maternal decidual tissues, and as such it may provide useful information concerning the interactions between EVTs and decidual cells and vessels during early gestation. (Lab Invest 2003, 83:1821-1828.

show abstract

Detection of class I MHC mRNA in subpopulations of first trimester cytotrophoblast cells by in situ hybridization

Cited by 28 publications

References 21 publications

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

DNA Alkylating Agents Alleviate Silencing of Class II Transactivator Gene Expression in L1210 Lymphoma Cells

A Novel In Vitro Model of Trophoblast-Mediated Decidual Blood Vessel Remodeling

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