Detection of clarithromycin-resistant Helicobacter pylori in frozen gastric biopsies from pediatric patients by a commercially available fluorescent in situ hybridization

Vega, Alba Edith; Alarcón, Teresa; Domingo, Diego; López-Brea, M

doi:10.1016/j.diagmicrobio.2007.06.020

Cited by 14 publications

(14 citation statements)

References 10 publications

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“…One of the advantages of FISH and PCR is the ability of determining clarithromycin resistance in frozen or formalin-fixed, paraffinembedded tissues whereas the E test (minimum inhibitory concentration method using a paper strip with antibiotic gradient) is dependent on bacterial growth [13, [103][104][105]117]. FISH was applied to 10 years of archived paraffin-embedded gastric biopsies of Italian children and clarithromycin resistance was found in 42% of these specimens with some patients harboring sensitive and resistant strains [13].…”

Section: Fluorescent In Situ Hybridization or Pcrmentioning

confidence: 99%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

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The array of tests that can be used for diagnosis of Helicobacter pylori infection is large, and it can be confusing to define which test to use particularly in children where results may not be comparable to those obtained in adult patients. Using PubMed, we reviewed the English literature from January 1999 to May 2009 to identify articles that determined sensitivity and specificity of H. pylori invasive and non-invasive diagnostic tests in children. We excluded articles that presented a review of the literature, abstracts, case reports, or series where children's results could not be separated from adult populations. Of the tissue based methods, rapid urease tests have better sensitivity than histology to detect presence of H. pylori; however, histology can detect the pathology associated with disease including gastritis, intestinal metaplasia, and other conditions that could be the cause of the child's symptoms. Culture of gastric tissues or stool has 100% specificity but sensitivity is low. Of the serologic tests, immunoblot has the best sensitivity. The urea breath tests have >75% sensitivity for detection of H. pylori before and after treatment. Immunoassays in stool using monoclonal antibodies have >95% sensitivity for detection of H. pylori before and after treatment. PCR testing can be performed in tissue and stool samples and can detect genes associated to antibiotic resistance. In summary, the current commercial non-invasive tests have adequate sensitivity and specificity for detecting the presence of H. pylori; however, endoscopy with histopathology is the only method that can detect H. pylori and lesions associated with the infection.

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Section: Fluorescent In Situ Hybridization or Pcrmentioning

confidence: 99%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

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“…3,4 In detail, PCR-based methods allow to identify even minimal traces of resistant strains among a large susceptible bacterial population (heteroresistance). 5 This peculiar ability could explain the discrepancies in the clarithromycin resistance rates between culture and PCR-based methods and frequent therapeutic failures in presence of clarithromycin susceptibility at the culture. 9 The need of multiple gastric biopsies taken in different gastric sites as been pointed out for bacterial culture, 10 but the biopsy sampling for PCR-based methods to assess H. pylori resistance has been poorly investigated.…”

Section: Discussionmentioning

confidence: 99%

“…the heteroresistance. 5 Such a status describes the coexistence of resistant and susceptible bacterial strains towards to a specific antibiotic in the same patient. Heteroresistance may be either intraniche (coexistent susceptible and resistant strains in the same gastric site) or interniche (separate susceptible and resistant strains in different gastric site, i.e.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori clarithromycin resistance assessment: are gastric antral biopsies sufficient?

Francesco

Giorgio

Ierardi

et al. 2012

Gastroenterol Insights

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Gastric biopsy sampling could affect accuracy of Helicobacter pylori clarithromycin resistance assessment due to coexistence of susceptible and resistant strains (i.e. heteroresistance) either in same gastric site (intraniche) or in two different gastric sites (interniche). This study aimed to assess differences in the H. pylori clarithromycin resistance prevalence in relation to the gastric biopsy sampling by using Taqman-real time polymerase chain reaction (PCR). The study enrolled 137 patients. Primary clarithromycin resistance was observed in 15 isolates exclusively in antrum, in 7 cases exclusively in gastric body, and in 3 patients in both gastric sites. The overall prevalence of clarithromycin resistance was 13.1% by using exclusively antral biopsies, and 18.2% by using biopsies from both gastric sites. Moreover, intra-niche heteroresistance was observed in 19 (76%) out of 25 patients harbouring resistant strains. Our data found a heterogeneous distribution of resistant H. pylori strains in the stomach. Similarly to culture, gastric biopsies from both antrum and gastric body are needed to increase the accuracy of PCR-based methods for clarithromycin resistance assessment.

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“…Bu tedavide başarı oranı % 90'lara ulaşmaktadır (7,11) . Bununla birlikte, özellikle klaritromisine karşı gelişen makrolid direnci, üçlü tedavide başarı-sızlığa yol açmaktadır (7,11,13,14) . Makrolid direncinin saptanmasında Antibiyotik gradiyent testi, disk difüzyon yöntemi, gerçek zamanlı polimeraz zincir reaksiyonu (PZR) ve floresan in situ hibridizasyon (FISH) yöntemi sıklıkla kullanıl-maktadır.…”

Section: Introductionunclassified

“…Bu mutasyonlar yapısal değişikliğe neden olur ve makrolidlerin bağlanması engellenir. Bakterinin 23S rRNA'sında A2115G, G2141A, A2141G, A2142G, A2143G, A2144G, A2143C, A2142T, T2717C, T2182C gibi farklı nokta mutasyonlar görül-müştür (7,9,14) . Dirençten sorumlu mutasyonlardan en sık görülenler A2143G ve A2144G olup, ender olarak A2142C, T2717C ve T2182C mutasyonlarına da rastlanmaktadır.…”

Section: Introductionunclassified

Determination of Clarithromycin Resistance in Helicobacter pylori by Molecular Method

İrvem¹,

Kolayli²,

Hülagü³

2015

Ankem Derg

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Detection of clarithromycin-resistant Helicobacter pylori in frozen gastric biopsies from pediatric patients by a commercially available fluorescent in situ hybridization

Cited by 14 publications

References 10 publications

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

Helicobacter pylori clarithromycin resistance assessment: are gastric antral biopsies sufficient?

Determination of Clarithromycin Resistance in Helicobacter pylori by Molecular Method

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