2016
DOI: 10.1016/j.saa.2016.06.052
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Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor

Abstract: Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The… Show more

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Cited by 46 publications
(21 citation statements)
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“…The platform provided the LOD of 0.03 pg/mL in ultrapure water, and this study demonstrated the versatility of the photonic materials as a nanobiosensor for detecting viruses. In another application, AuNPs and the cadmium-telluride quantum dots (CdTe-QDs) were combined to fabricate a SPR energy transfer-based biosensor for the detection of Citrus tristeza ―an agent of the most detrimental disease named tristeza [ 147 ]. For this purpose, after extraction of viruses from plants, they used and combined AuNPs/CdTe-QDs for the labeling of antibodies and antigens.…”
Section: Smart Materials For Sensor Technologiesmentioning
confidence: 99%
“…The platform provided the LOD of 0.03 pg/mL in ultrapure water, and this study demonstrated the versatility of the photonic materials as a nanobiosensor for detecting viruses. In another application, AuNPs and the cadmium-telluride quantum dots (CdTe-QDs) were combined to fabricate a SPR energy transfer-based biosensor for the detection of Citrus tristeza ―an agent of the most detrimental disease named tristeza [ 147 ]. For this purpose, after extraction of viruses from plants, they used and combined AuNPs/CdTe-QDs for the labeling of antibodies and antigens.…”
Section: Smart Materials For Sensor Technologiesmentioning
confidence: 99%
“…The detection limit was found to be 0.27 nM, which is lower than ELISA and other immunoassay techniques for CTV detection. 84,85 In another example of CTV detection, a microfluidic electrochemical device (μFED) was proposed by Freitas et al 86 The μFED included an array of eight screen-printed electrodes fabricated. Poly(diallyldimethylammonium chloride solution) (PDDA) was applied to electrode surface to create a positively charged film.…”
Section: Antibody-based Sensors For Viral Antigen and Particle Detectionmentioning
confidence: 99%
“… Sun et al (2008) discussed the sensor labeled with the 23-mer oligonucleotide derived from the CaMV 35S promoter on a PbS nanoparticle. Investigations into the sensor labeled with the antibody derived from CTV coat protein on CdTe QDs ( Safarnejad et al, 2017 ; Shojaei et al, 2016a ) and the sensor labeled with the GVA antibody derived from GVA coat protein on the ZnO films ( Tereshchenko et al, 2017 ) have followed. The details of QD-based biosensors for the detection of plant viruses are summarized in Table 3 .…”
Section: Detection Of Plant Viruses Using Biosensorsmentioning
confidence: 99%
“…In fact, using antibody derived from CTV coat protein, alternative types of biosensors were reported to enhance the specificity and sensitivity of fluorescence resonance energy transfer (FRET)-based biosensor. Shojaei et al (2016a) reported the complex sensor using the mixture of CdTe quantum dots and gold nanoparticles (AuNPs) labeled with an antibody derived from CTV coat protein. Safarnejad et al (2017) reported on the donor-acceptor complex sensor; QDs conjugated with antibody derived from CTV coat protein and rhodamine 123 conjugated with the recombinant coat protein of the CTV were used as donor and acceptor, respectively ( Fig.…”
Section: Detection Of Plant Viruses Using Biosensorsmentioning
confidence: 99%