Detection of Citrus psorosis virus in the northwestern citrus production area of Argentina by using an improved TAS-ELISA

Resistance to Citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein–RNA silencing

“…Triple-sandwich immunoassay (TAS-ELISA-HRP) was performed as previously described by Zanek et al (2006) using total protein extracted from 200 mg of leaf tissue. In these experiments samples were considered positive for CPsV when its OD is ≥2.7 times the healthy control in the TAS-ELISA.…”

Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus‐derived Resistance against Psorosis A and Psorosis B

“…The progress of the infection in the challenged plants was evaluated by RT‐PCR and Triple‐Antibody Sandwich Immunoassay (TAS‐ELISA) as described previously (Zanek et al. 2006).…”

Recent advances in Citrus psorosis virus

“…The tools used to detect CPsV in citrus trees are mainly based on using indicator plants and on laboratory tests including enzyme-linked immunosorbent assay (ELISA) [20,33,80]. After CPsV sequences became available, molecular tests based on RT-PCR and dot blot or tissue-print hybridization were also developed for CPsV detection [5,20,26,28,32,55,57,67].…”

“…Its substrate is the chromogenic 3, 3 0 , 5, 5 0 -tetramethyl benzidine dihydrochloride (TMB) or o-phenylenediamine (OPD). Zanek et al [80] describe the use of HRP for detecting the presence of the virus in the field samples and in the transgenic sweet oranges expressing very low amounts of viral CP. HRP is more reliable, faster and more sensitive than AP with no significant variability and has a lower detection limit, since the AP reaction takes from 17 to 24 h instead of 30 min for the TAS-ELISA-HRP.…”

“…Usually, infected trees are not killed by the virus but slowly decline and eventually become unproductive, causing damage to citrus industry in the Mediterranean basin, and in some areas of South America. In the 1980s, in Argentina and Uruguay it was a serious disease causing annual losses of about 5 % of trees [8] and the disease is still present as recently reported by Zanek et al [80]. Another earlier investigation of Paul W. Moore in California, published in 1957, was conducted in order to find out the approximate bearing efficiency of an orchard in which psorosis appears to be the principal cause of crop reduction.…”