Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture

Yang, Chun‐Seok; Kim, In-Hwan; Chae, Hyun‐Dong; Kim, Dae‐Dong; Jeon, Chang‐Ho

doi:10.21873/invivo.12404

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Transwell dish-based 3D models from prostate CR cancer cells, organoid culture of prostate CR cancer cells, and multicellular spheroids of laryngeal, lung, and salivary cancer CR cells have been generated ( 50 , 51 , 80 , 89 ). Additionally, the CR technique can be used for direct isolation of CTCs from the blood ( 90 , 91 ).…”

Section: Discussionmentioning

confidence: 99%

Conditionally Reprogrammed Cells and Robotic High-Throughput Screening for Precision Cancer Therapy

Alkhilaiwi

2021

Front. Oncol.

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Cancer is a devastating disease that takes the lives of millions of people globally every year. Precision cancer therapy is based on a patient’s tumor histopathology, expression analyses, and/or tumor RNA or DNA analysis. Only 2%–20% of patients with solid tumors benefit from genomics-based precision oncology. Therefore, functional diagnostics and patient-derived cancer models are needed for precision cancer therapy. In this review, we will summarize the potential use of conditional cell reprogramming (CR) and robotic high-throughput screening in precision cancer medicine. Briefly, the CR method includes the co-culturing of irradiated Swiss-3T3-J2 mouse fibroblast cells alongside digested primary non-pathogenic or pathogenic cells with the existence of Rho-associated serine–threonine protein kinase inhibitor called Y-27632, creating an exterior culture environment, allowing the cells to have the ability to gain partial properties of stem cells. On the other hand, quantitative high-throughput screening (qHTS) assays screen thousands of compounds that use cells in a short period of time. The combination of both technologies has the potential to become a driving force for precision cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Conditionally Reprogrammed Cells and Robotic High-Throughput Screening for Precision Cancer Therapy

Alkhilaiwi

2021

Front. Oncol.

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“…Although the number of samples was too small for a definite evaluation, a high CTC count was associated with colony formation ( Figure 2 ). Approximately 30 studies on short-term cultures of CTC were subsequently published ( Table 3 and Table 4 [ 7 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]). Ten of these studies were by the same research group and used the same method [ 35 , 36 , 37 , 38 , 39 , 40 , 44 , 45 , 47 , 51 ].…”

Section: Short-term Culturementioning

confidence: 99%

Cell Lines of Circulating Tumor Cells: What Is Known and What Needs to Be Resolved

Shimada

Sudo

Akamatsu

et al. 2022

JPM

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The importance of circulating tumor cells (CTC) is well recognized. However, the biological characteristics of CTC in the bloodstream have not yet been examined in detail, due to the limited number of CTC cell lines currently available. Thirty-nine CTC cell lines were reported by 2021. For successful cell culturing, these CTC cell lines were reviewed. Previous studies on short-term cultures of CTC also analyzed approaches for establishing the long-term culture of CTC. Negative selection, hypoxic conditions, three-dimensional conditions, and careful management are preferable for the long-term culture of CTC. However, the establishment of CTC cell lines is dependent on the specific characteristics of each cell type. Therefore, a method to establish CTC cell lines has not yet been developed. Further efforts are needed to resolve this issue.

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State of the Art in the Propagation of Circulating Tumor Cells

Jian-yun

Pohlmann

Schlegel

et al. 2023

Current Cancer Research

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Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture

Cited by 4 publications

References 29 publications

Conditionally Reprogrammed Cells and Robotic High-Throughput Screening for Precision Cancer Therapy

Conditionally Reprogrammed Cells and Robotic High-Throughput Screening for Precision Cancer Therapy

Cell Lines of Circulating Tumor Cells: What Is Known and What Needs to Be Resolved

State of the Art in the Propagation of Circulating Tumor Cells

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