Detection of circulating antigen in sera of Fasciola gigantica-infected cattle with antibodies reactive with a Fasciola-specific 88-kDa antigen

Fagbemi, Benjamin O.; Obarisiagbon, I. O.; Mbuh, J.V.

doi:10.1016/0304-4017(94)00718-r

Cited by 45 publications

(18 citation statements)

References 15 publications

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“…In our study, circulating antigens diminished to uninfected values at 2±4 wpt with triclabendazole, in agreement with data from Fagbemi et al (1995), who proved that circulating antigens were reduced at 3 wpt with oxyclozanide in sheep infected with F. gigantica. We observed an increment in the values of circulating antigens at 6 wpt with triclabendazole.…”

Section: Discussionsupporting

confidence: 81%

Effect of fasciolicides on the antigenaemia in sheep naturally infected with Fasciola hepatica

Sánchez-Andrade

Paz-Silvạ

et al. 2001

Parasitology Research

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A study was developed to evaluate the influence of triclabendazole (Fasinex) and netobimin (Hapasil) the antigenaemia in sheep naturally infected with Fasciola hepatica during 16 weeks. A sandwich-ELISA (enzyme-linked immunosorbent assay) using a rabbit polyclonal IgG antibody to F. hepatica antigens was employed and the data obtained were compared to those from coprological and indirect-ELISA techniques. Triclabendazole reduced the values of circulating antigens at weeks 2-4 post-treatment and faecal output at weeks 2-8 post-treatment, but antibodies showed positive values until the end of the study. Netobimin did not reduce circulating antigens of the trematode nor egg-excretion; and IgG antibodies did not decrease throughout the study.

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Section: Discussionsupporting

confidence: 81%

Effect of fasciolicides on the antigenaemia in sheep naturally infected with Fasciola hepatica

Sánchez-Andrade

Paz-Silvạ

et al. 2001

Parasitology Research

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“…gigantica was detected in the sera of experimentally infected cattle at the first week post infection [ 10 ]. Fagbemi et al [ 9 ] could detect circulating 88 kDa antigen in the sera of cattle experimentally infected with F . gigantica during the 2nd and 3rd weeks after infection.…”

Section: Discussionmentioning

confidence: 99%

Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease

2016

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BackgroundTropical fasciolosis caused by Fasciola gigantica infection is one of the major diseases infecting ruminants in the tropical regions of Africa and Asia including Thailand. Parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Therefore, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden.MethodsIn this study, we have produced a monoclonal antibody (MoAb) against recombinant F. gigantica cathepsin L1 (rFgCatL1), and developed both sandwich enzyme-linked immunosorbent assay (sandwich ELISA) and immunochromatographic (IC) test for rapid detection of circulating cathepsin L1 protease (CatL1) in the sera from mice experimentally and cattle naturally infected with Fasciola gigantica. MoAb 4E3 and biotinylated rabbit anti-recombinant CatL1 antibody were selected due to their high reactivities and specificities.ResultsThe lower detection limits of sandwich ELISA and IC test were 3 pg/ml and 0.256 ng/ml, respectively. Sandwich ELISA and IC test could detect F. gigantica infection from day 1 to 35 post infection. In experimental mice, the sensitivity, specificity and accuracy were 95%, 100% and 98.6% (for sandwich ELISA), and 93%, 100% and 98.2% (for IC test), while in natural cattle they were 98.3%, 100% and 99.5% (for sandwich ELISA), and 96.7%, 100% and 99.1% (for IC test).ConclusionsThese two assay methods showed high efficiencies and precisions for diagnosis of fasciolosis by F. gigantica.

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“…Of note, all serologic tests based on the 27-kDa antigen were developed and optimized with an emphasis on the detection of antibodies to F. hepatica (12,13,44,45). However, the direct detection of Fasciola antigens secreted by the living flukes has apparent advantages over antibody detection tests, in that antigenemia implies active infection, and this approach has the ability to assess efficacy of chemotherapy and determine the effectiveness of future vaccines (24,(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

Immunodetection of Fasciola gigantica Circulating Antigen in Sera of Infected Individuals for Laboratory Diagnosis of Human Fascioliasis

Attallah¹,

Bughdadi

El-Shazly

et al. 2013

Clin. Vaccine Immunol.

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c Currently, the laboratory diagnosis of human fascioliasis is based on the parasitological examination of parasite eggs in stool specimens and serological detection of specific antibodies in serum samples, which are often unreliable diagnostic approaches. Ideally, a sensitive and specific diagnostic test for Fasciola infection should be based on the detection of circulating Fasciola antigen, which implies active infection. Here, a 27-kDa-molecular-mass antigen was identified in a Fasciola gigantica adult worm antigen preparation, excretory-secretory products, and sera from F. gigantica-infected individuals, and it was not detected in antigenic extracts of other parasites and sera from noninfected individuals. The target antigen was isolated and partially characterized as a protein. Immunoperoxidase staining located the target epitope within teguments and guts of F. gigantica adult worms. The performance characteristics of a newly developed enzyme-linked immunosorbent assay (ELISA) based on F. gigantica circulating antigen detection in serum (FgCA-27 ELISA) were investigated using sera of 120 parasitologically diagnosed F. gigantica-infected individuals and 80 noninfected individuals. The area under the receiving operating characteristic (ROC) curve (AUC) for ELISA was significantly high (AUC ‫؍‬ 0.961, P < 0.0001) for discriminating Fasciola-infected and noninfected individuals. The developed assay showed high degrees of sensitivity, specificity, and efficiency (>93%), and a significant correlation (r ‫؍‬ 0.715, P < 0.0001) between antigen level and parasite egg count was shown. In conclusion, a 27-kDa Fasciola antigen was identified in sera of F. gigantica-infected individuals. A highly sensitive and specific Fasciola antigen detection assay, FgCA-27 ELISA, was developed for laboratory diagnosis of human fascioliasis.

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Detection of circulating antigen in sera of Fasciola gigantica-infected cattle with antibodies reactive with a Fasciola-specific 88-kDa antigen

Cited by 45 publications

References 15 publications

Effect of fasciolicides on the antigenaemia in sheep naturally infected with Fasciola hepatica

Effect of fasciolicides on the antigenaemia in sheep naturally infected with Fasciola hepatica

Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease

Immunodetection of Fasciola gigantica Circulating Antigen in Sera of Infected Individuals for Laboratory Diagnosis of Human Fascioliasis

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