Detection of Circulating Antibodies in Cutaneous Leishmaniasis by Enzyme-Linked Immunosorbent Assay (ELISA) *

Roffi, J; Dedet, Jean-Pierre; Desjeux, Philippe; Garré, M.

doi:10.4269/ajtmh.1980.29.183

Cited by 41 publications

(17 citation statements)

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“…Prior research attempts employing nonrecombinant antigens in the design of serological tests for leishmaniasis were limited by problems with sensitivity, specificity, and test reproducibility (1,11,23). Reasons for these limitations remain elusive but are likeliest attributable to physical and chemical techniques used in antigen preparation.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis

et al. 2002

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Leishmaniasis causes significant morbidity and mortality in areas where it is endemic. In areas where it is nonendemic, global travel and increased incidence of the disease in human immunodeficiency virus and intravenous-drug user populations are also causes for concern. The unavailability of rapid and reliable tests for diagnosis of the various leishmaniases makes patient management difficult. We have developed an enzymelinked immunosorbent assay (ELISA) that can detect immunoglobulin M (IgM) and IgG antibodies in patients with visceral and cutaneous leishmaniasis. These practical assays are based on soluble antigens from promastigotes cultivated in a protein-free medium. In preliminary studies, 129 visceral (Brazil, Italy, North Africa, and Nepal) and 143 cutaneous (Brazil) leishmaniasis patients with controls were tested. Overall, the tests showed a sensitivity of 95.1%. In addition, the ELISA correctly identified 42 sera from Brazilian dogs with canine leishmaniasis and 10 healthy controls. Serological tests for the various clinical manifestations of leishmaniasis could be useful epidemiological and patient management tools in populations of areas of endemicity and nonendemicity.

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Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis

et al. 2002

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“…ODDONE et al 8 , in a study of the usefulness of diagnostic tests applied to mucous leishmaniasis, detected 100% positivity for MST, 88% for PCR, 78.6% for IIFR, 16% for parasite culture, and 4% for histopathology, but did not use ELISA. In the evaluation of ELISA using an L. tropica major antigen in patients with cutaneous leishmaniasis caused by this parasite, ROFFI et al 9 reported 97% positivity for the test. The Health Ministry 7 estimates 90% positivity for MST and 70% for IIFR in ulcerated lesions caused by L. (V).…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of an immunoenzymatic test for detection of ant-L. brasiliensis antibodies compared to other tests used for the diagnosis of American cutaneous leishmaniasis

Ferreira

Roselino

Nascimento

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe diagnosis of American cutaneous leishmaniasis (ACL) is frequently based on clinical and epidemiological data associated with the results of laboratory tests. Some laboratory methods are currently being applied for the diagnosis of ACL, among them the indirect immunofluorescence reaction (IIFR), the Montenegro skin test (MST), histopathological examination, and the polymerase chain reaction (PCR). The performance of these methods varies in a considerable proportion of patients. After the standardization of an immunoenzymatic test (ELISA) for the detection of IgG in the serum of patients with ACL using a crude Leishmania braziliensis antigen, the results obtained were compared to those of other tests routinely used for the diagnosis. The tests revealed the following sensitivity, when analyzed separately: 85% for ELISA IgG, 81% for PCR, 64.4% for MST, 58.1% for IIFR, and 34% for the presence of parasites in the biopsy. ELISA was positive in 75% of patients with ACL presenting a negative MST, in 84.8% of ACL patients with negative skin or mucous biopsies for the presence of the parasite, and in 100% of cases with a negative PCR. Thus, ELISA presented a higher sensitivity than the other tests and was useful as a complementary method for the diagnosis of ACL.

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“…The enzyme-linked immunosorbent assay (ELISA) has proved to be at least as sensitive and specific as IFAT and is suitable for such surveys (Evans et al 1990, Paranhos-Silva et al 1996, Braga et al 1998. Cross-reactivity with other canine parasites and bacterial pathogens has also been reported for ELISA (Roffi et al 1980, Mancianti et al 1996. Crude antigen preparations made from whole promastigotes or their soluble extracts limit standardization of the tests and reproducibility of the results (Hommel et al 1978, Mohammed et al1986, Burns et al 1993.…”

Section: Abstract: Canine Visceral Leishmaniasis -Leishmania (Leishmmentioning

confidence: 99%

Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis

Rosário

Genaro²,

França-Silva

et al. 2005

Mem. Inst. Oswaldo Cruz

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The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in 106 (92.2%) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 1:40 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100%). Specificity was higher than 96% for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88%). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97) . Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however. Key words: canine visceral leishmaniasis -Leishmania (Leishmania) chagasi -diagnosis -recombinant antigensVisceral leishmaniasis (VL) is a zoonosis caused by Leishmania (Leishmania) chagasi and transmitted by the phlebotomine sand fly Lutzomyia (Lutzomyia) longipalpis in Brazil. VL occurs in both rural and urban areas and has become a serious public health problem in several large Brazilian cities in recent decades (Arias et al. 1996). Domestic dogs play an important role in VL maintenance in man-made environments by serving as reservoirs of the parasite (Deane & Deane 1962). In Brazil, canine visceral leishmanisis (CVL) prevalence ranges from 1.9 to 35% in endemic areas (Evans et al. 1990, Nunes et al. 1991, França-Silva et al. 2002. The parasite can be isolated by means of tissue biopsies in 40-50% of dogs with positive immunofluorescence titres (Lanotte et al. 1979, Pozio et al. 1981 as well as asymptomatic animals . Consequently, asymptomatic and symptomatic infected dogs are both infective to the sand fly vectors (Molina et al. 1994).Recommended VL control strategies in Brazil involve systematic treatment of human cases, elimination of seropositive dogs and residual spraying of houses and animal shelters with insecticides (Vieira & Coelho 1998 high proportion of asymptomatic dogs, serological methods are essential for CVL diagnosis. Although the immunofluorescent antibody test (IFAT) is the most widespread diagnostic method, cross-reactivity with other diseases and low sensitivity in detecting asymptomatic dogs are the main limitations of this technique. In addition, IFAT is not readily adaptable to large-scale seroepidemiologi...

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Detection of Circulating Antibodies in Cutaneous Leishmaniasis by Enzyme-Linked Immunosorbent Assay (ELISA) *

Cited by 41 publications

References 0 publications

Enzyme-Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis

Enzyme-Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis

Sensitivity of an immunoenzymatic test for detection of ant-L. brasiliensis antibodies compared to other tests used for the diagnosis of American cutaneous leishmaniasis

Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis

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