Detection of chromosomes carrying genes for seed dormancy of wheat using the backcross reciprocal monosomic method

Miura, H.; Sato, Nariko; Kato, Kiyoaki; Amano, Y.

doi:10.1046/j.1439-0523.2002.741382.x

Cited by 26 publications

(15 citation statements)

References 24 publications

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“…These QTLs were derived from white wheat cultivars and are not related to any Red genes; therefore, they can be used to breed white wheat for improved PHS resistance. Several recent studies demonstrated a major QTL for PHS on the short arm of 3A of the Japanese red wheat cultivar Zen (Miura et al 2002;Mori et al 2005;Osa et al 2003), but whether it exists in white wheat remains unknown. In the current study, one major QTL, QPhs.pseru-3AS, was repeatedly detected on chromosome 3A of a white wheat cultivar Rio Blanco.…”

Section: Discussionmentioning

confidence: 99%

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

et al. 2008

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Pre-harvest sprouting (PHS) of wheat is a major problem that severely limits the end-use quality of Xour in many wheat-growing areas worldwide. To identify quantitative trait loci (QTLs) for PHS resistance, a population of 171 recombinant inbred lines (RILs) was developed from the cross between PHS-resistant white wheat cultivar Rio Blanco and PHS-susceptible white wheat breeding line NW97S186. The population was evaluated for PHS in three greenhouse experiments and one Weld experiment. After 1,430 pairs of simple sequence repeat (SSR) primers were screened between the two parents and two bulks, 112 polymorphic markers between two bulks were used to screen the RILs. One major QTL, QPhs.pseru-3AS, was identiWed in the distal region of chromosome 3AS and explained up to 41.0% of the total phenotypic variation in three greenhouse experiments. One minor QTL, QPhs.pseru-2B.1, was detected in the 2005 and 2006 experiments and for the means over the greenhouse experiments, and explained 5.0-6.4% of phenotypic variation. Another minor QTL, QPhs.pseru-2B.2, was detected in only one greenhouse experiment and explained 4.5% of phenotypic variation for PHS resistance. In another RIL population developed from the cross of Rio Blanco/NW97S078, QPhs.pseru-3AS was signiWcant for all three greenhouse experiments and the means over all greenhouse experiments and explained up to 58.0% of phenotypic variation. Because Rio Blanco is a popular parent used in many hard winter wheat breeding programs, SSR markers linked to the QTLs have potential for use in high-throughput marker-assisted selection of wheat cultivars with improved PHS resistance as well as Wne mapping and map-based cloning of the major QTL QPhs.pseru-3AS.

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Section: Discussionmentioning

confidence: 99%

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

et al. 2008

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“…Previously, in "Zen", chromosomes carrying genes for grain dormancy were detected in comparison with "CS" homologue by Miura et al (2002). According to their results, chromosome 3B of "Zen" has produced very low germination % although the difference between "CS" and "Zen" was not significant due to the fact that "CS" homologue also produced low germinations.…”

Section: Discussionmentioning

confidence: 99%

“…According to a previous study of Miura et al (2002), 7B in "Zen" was identified as a possible site for the availability of dormancy genes. Hence, it can be speculated that chromosome 7B might contain important genes affecting grain dormancy validating the QTL detected on 7B of the present study in "Zen" x "Spica" cross.…”

Section: Discussionmentioning

confidence: 99%

“…Twenty red RILs were also developed from the same cross to be used as the control. F 2 plants from the "Zen" x "Spica" cross segregated as 15 red-grained to 1 whitegrained, as "Zen" carries the dominant red alleles at the R-B1 and R-D1 loci on chromosomes 3B and 3D, respectively, and the white R-A1a allele on chromosome 3A (Miura et al, 2002). "Spica" expresses white-grained color because all of the R genes on group 3 chromosomes are recessive in this line with the genotype of R-A1a : R-B1b : R-D1a.…”

Section: Methodsmentioning

confidence: 99%

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Identification of Grain Dormancy QTLs in a White-Grained Wheat Population Derived from ‘Zen’ x ‘Spica’ Cross

Kottearachchi

Takao²,

Kato³

et al. 2010

Trop Agric Res & Ext

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White-grained wheat is usually more susceptible to preharvest sprouting (PHS). Accumulation of color independent grain dormancy QTLs is one of the promising ways of developing PHS tolerance in whitegrained wheat. A white grained recombinant inbred line (RIL) population was developed from a cross between high dormant Japanese red grained wheat, 'Zenkoujikomugi' ('Zen'), and a white cultivar 'Spica'. White-grained RILs demonstrated a severe variation proving that many genes have involved in maintaining grain dormancy. Hence, to identify those dormancy QTLs of 'Zen' x 'Spica', whole genome was assessed with SSR and EST markers. Five novel grain dormancy QTLs, linked with Xwocs207, Xcfa2163, Xbarc243, Xbarc44 and Xgwm577 markers were detected located on chromosomal arms 3BS, 5AL, 5BL, 5DL and 7BL, respectively. In all instances, allele contributed to comparatively higher dormancy was derived from 'Zen'. The QTL linked with Xwocs207 marker appeared to be extremely effective, stable and reliable. Usually QTLs rendering minor effects were difficult to be detected if the phenotypic variation of the population was affected by major red color genes. In the present study, since only the white-grained RILs were used, minor QTLs were also able to be investigated.

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“…O principal componente da variação genética da germinação na pré-colheita de trigo parece ser o grau de dormência, que necessita ser avaliado como mecanismo de possível transferência para outros cultivares (Flintham, 2000;Bassoi, 2002;Miura et al, 2002;Osa et al, 2003). Assim, a identificação de cultivares tolerantes à germinação na espiga pode ser uma importante ferramenta na seleção de genótipos para os programas de melhoramento genético em trigo.…”

Section: Introductionunclassified

Pré-esfriamento para superação da dormência de sementes de trigo colhidas na época da maturidade

Franco¹,

Pinto

Scapim

et al. 2009

Rev. bras. sementes

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alessanDro De lucca e Braccini 3 RESUMO -O melhoramento genético de trigo tem gerado número expressivo de cultivares que diferem quanto ao potencial de germinação de sementes ainda presas à espiga. Para avaliar essas diferenças, foi conduzida pesquisa com sementes colhidas em dois locais, Palotina e Cascavel, Estado do Paraná, incluindo doze cultivares em delineamento blocos casualizados, com quatro repetições. As sementes foram colhidas na época da maturidade fisiológica, sendo uma das amostras submetida a pré-esfriamento para superação da dormência e a outra representou a testemunha não tratada. As sementes que não germinaram foram submetidas ao teste de tetrazólio para confirmar a ocorrência de dormência. Os resultados foram submetidos a análise da variância e à posterior determinação da significância da diferença entre médias. A análise dos resultados permitiu verificar que, nas duas localidades, os percentuais de germinação de sementes de 'Frontana' e de 'IAPAR 53', foram extremamente baixos, indicando a existência de maior número de sementes dormentes. O método de superação de dormência para amostras provenientes de Palotina não foi eficiente para permitir a completa germinação das sementes. Por outro lado, o resfriamento de amostras colhidas em Cascavel permitiu a germinação das sementes de todas as cultivares. A manifestação da dormência, detectada em 'Frontana' e em 'IAPAR 53', é considerada eficiente para impedir a germinação indesejável das sementes ainda presas à espiga, na época da maturidade fisiológica.Termos para indexação: Triticum aestivum, fisiologia de semente, germinação, tetrazólio. PRECHILING AS A TREATMENT TO OVERCOME DORMANCY OF WHEAT SEEDS HARVESTED AT PHYSIOLOGICAL MATURITYABSTRACT -The wheat breeding program has generated an expressive number of cultivars which differ in sprouting rate of seed still attached to the spike. In order to assess these differences, an experiment was carried out with seeds harvested from two locations, Palotina and Cascavel, in Paraná State, Brazil, consisting of twelve cultivars in a randomized block design with four replications. Seeds were harvested at physiological maturity, in which one sample was submitted to a pre-chilling

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Detection of chromosomes carrying genes for seed dormancy of wheat using the backcross reciprocal monosomic method

Cited by 26 publications

References 24 publications

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

Identification of Grain Dormancy QTLs in a White-Grained Wheat Population Derived from ‘Zen’ x ‘Spica’ Cross

Pré-esfriamento para superação da dormência de sementes de trigo colhidas na época da maturidade

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