Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen–Friedenreich antigen-specific Amaranthus caudatus lectin

Akiyoshi, Hideo; Sugii, Shunji; Nahid, M. Abu; Sone, Katsuhito; Tanaka, Toshiyuki; Cao, Zheng; Li, Yijyun; Aoki, Mica; Takenaka, Shigeo; Shimada, Terumasa; Shimizu, Junichiro; Kiyomiya, Ken-ichi; Ohashi, Fumihito

doi:10.1016/j.vetimm.2011.08.024

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…Akiyoshi et al developed a two-site enzyme immunoassay by using both lectin for capturing CgA protein regardless of the species and species-specific labeled antibody (Akiyoshi et al, 2011). However in their study, plasma bCgA levels were profiled.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, few studies have reported on the assaying plasma bCgA concentration in bovine (Akiyoshi et al., 2011). Akiyoshi et al developed a two‐site enzyme immunoassay by using both lectin for capturing CgA protein regardless of the species and species‐specific labeled antibody (Akiyoshi et al., 2011). However in their study, plasma bCgA levels were profiled.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, few studies have reported on the assaying plasma bCgA concentration in bovine (Akiyoshi et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Development of enzyme immunoassay for chromogranin A (CgA) and profiling of plasma CgA concentrations in the cow

Saito

Ido-Matsumoto

Kumazaki

et al. 2021

Animal Science Journal

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The objectives of this study were to establish and characterize a homologous immunoassay for bovine chromogranin A (bCgA) and to profile plasma bCgA concentrations during early pregnancies. We synthesized oligopeptide corresponding to the amino acid sequence 341–355 of bCgA for immunizing rabbits and peptide corresponding to the amino acid sequence 336–365 of bCgA for both a biotinylated tracer and reference standards. Recombinant bCgA protein was also generated in Escherichia coli lysate. Dose‐dependent displacement curves were obtained from 1 to 1,000 nM of the reference standards. The displacement curves showed a good relationship between the reference standards of the synthetic peptide and the serially diluted plasma sample or recombinant bCgA protein generated in the present study. The assay sensitivity defined as the value of two standard deviations below the zero standard was calculated as 0.46 nM. The intraassay and interassay coefficients of variation were 6.48% and 13.4%, respectively. Changes in the plasma bCgA concentrations in early pregnancies undulated in nonpregnant animals. The results of the present study suggest that assaying plasma bCgA concentrations could be utilized as measures to evaluate the physiological status of cattle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Development of enzyme immunoassay for chromogranin A (CgA) and profiling of plasma CgA concentrations in the cow

Saito

Ido-Matsumoto

Kumazaki

et al. 2021

Animal Science Journal

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“…CGA has also been measured in plasma samples and adrenal extract from dogs [17,18]. Salivary concentrations have been measured in dogs [19].…”

Section: Discussionmentioning

confidence: 99%

Chromogranins can be measured in samples from cats and dogs

et al. 2014

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BackgroundMethods for objective evaluation of stress in animals are important, but clinically difficult. An alternative method to study the sympathetic activity may be to investigate Chromogranin A (CGA), Chromogranin B (CGB) and Secretogranin II (SG2). The aim of this study was to investigate the cross-reactivity of CGA, CGB and SG2 between man, cat and dog and to explore possibilities to measure these proteins in samples from cats and dogs.ResultsAdrenal gland extracts from feline and canine species were measured by region-specific radioimmunoassays in different dilution steps to explore possible inter species cross reactivity. High cross reactivity was found for cats in the CGA17-38, CGA324-337, CGA361-372, CGB and SG2 assays. High cross reactivity was found for dogs in the CGA17-38, CGA361-372, CGB and SN assays. The method measuring the intact CGA was not useful for measurements in cats and dogs.ConclusionsRegion-specific assays measuring defined parts of CGA, CGB and SG2 can be used for measurements in samples from cats and dogs. These results are promising and will allow for further studies of these proteins as possible clinical biomarkers in cats and dogs.

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“…Öåé aeå ëåêòèí âèÿâèâ âèá³ðêîâó ñïîð³äíåí³ñòü äî êë³òèí íåðâîâîãî ãðåáåíÿ, ÿê³ ó ïîäàëüøîìó äèôåðåíö³þâàëèñü ó íåéðîåíäîêðèíîöèòè (ó òîìó ÷èñë³ äî õðîìàô³-íîöèò³â íàäíèðêîâèõ çàëîç), àëå íå âçàºìîä³ÿâ ç êë³òèíàìè ñóòî íåéðîíàëüíî¿ äèôåðåíö³àö³¿ (íåéðîí³â ñèìïàòè÷íèõ ãàíãë³¿â) ùóðà [54]. Ëåêòèí Amaranthus caudatus áóëî âèêîðèñòàíî äëÿ îòðèìàííÿ õðîìîãðàí³íó ç åêñòðàêò³â íàäíèðíè-ê³â ð³çíèõ âèä³â òâàðèí [55].…”

Section: îãëÿäè ë³òåðàòóðèunclassified

Application of lectin histochemistry methods for the investigation of influences of thyroid hormones imbalance on the organism

Lutsyk

Yashchenko

2018

AML

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Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen–Friedenreich antigen-specific Amaranthus caudatus lectin

Cited by 4 publications

References 19 publications

Development of enzyme immunoassay for chromogranin A (CgA) and profiling of plasma CgA concentrations in the cow

Development of enzyme immunoassay for chromogranin A (CgA) and profiling of plasma CgA concentrations in the cow

Chromogranins can be measured in samples from cats and dogs

Application of lectin histochemistry methods for the investigation of influences of thyroid hormones imbalance on the organism

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