Detection of chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and milk using a surface plasmon resonance biosensor and Qflex® kit chloramphenicol

Ferguson, Julie; Baxter, Andrew; Young, Paul; Kennedy, Glenn; Elliott, Chris; Weigel, Stefan; Gatermann, Robert; Ashwin, Helen; Stead, Sara; Sharman, Matthew

doi:10.1016/j.aca.2004.11.042

Cited by 141 publications

(54 citation statements)

References 7 publications

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“…Changes in antibody crossreactivity are not unusual as matrix has been found in the past to interfere with cross-reactivity. 41,42 Specificity studies determined that the antibody did not cross-react with gymnodimine, yessotoxin, pectenotoxin, or azaspiracid. This was an important finding as many of these toxins can be coextracted with the OA family of toxins from contaminated shellfish and yessotoxin can interfere with the results of the MBA.…”

Section: Resultsmentioning

confidence: 99%

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Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

et al. 2009

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A rapid analytical optical biosensor-based immunoassay was developed and validated for the detection of okadaic acid (OA) and its structurally related toxins from shellfish matrix. The assay utilizes a monoclonal antibody which binds to the OA group of toxins in order of their toxicities, resulting in a pseudofunctional assay. Single-laboratory validation of the assay for quantitative detection of OA determined that it has an action limit of 120 μg/kg, a limit of detection of 31 μg/kg, and a working range of 31−174 μg/kg. The midpoint on the standard matrix calibration curve is 80 μg/kg, half the current regulatory limit. Inter- and intra-assay studies of negative mussel samples spiked with various OA concentrations produced average coefficient of variation (CV) and standard deviation (SD) values of 7.9 and 10.1, respectively. The assay was also validated to confirm the ability to accurately codetect and quantify dinophysistoxin-1 (DTX-1), DTX-2, and DTX-3 from shellfish matrix. Alkaline hydrolysis was not required for the detection of DTX-3 from matrix. Excellent correlations with the data generated by the biosensor method and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were obtained using a certified reference material (R 2 = 0.99), laboratory reference material, and naturally contaminated mussel samples (R 2 = 0.97). This new procedure could be used as a rapid screening procedure replacing animal-based tests for DSP toxins.

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Section: Resultsmentioning

confidence: 99%

“…[37][38][39][40][41][42][43][44][45][46] This labelfree, automated analysis technique combines the high affinity of biochemical interactions with low limits of detection producing rapid and reliable results. Minimal amounts of analyte are required to produce the chip surfaces, and these surfaces can be reused many times without loss of activity.…”

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confidence: 99%

Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

et al. 2009

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“…They produce rapid and reliable results with minimal sample preparation and use of analyte (Elliott et al, 1999;Gaudin et al, 2001;Gustavsson et al, 2002;Haasnoot et al, 2002;Mello and Kubota, 2002;Samsonova et al, 2002;Ferguson et al, 2002Ferguson et al, , 2005Gaudin et al, 2005;Traynor et al, 2006;Mauriz et al, 2006;Llamas et al, 2007;Campbell et al, 2007;Connolly et al, 2007, Campas et al, 2007. This technology allows for real time, automated analysis combining the high affinity of biochemical interactions with low limits of detection and is characterised by its simplicity of use.…”

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Stewart

Elliott

Walker³

et al. 2009

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a b s t r a c tOkadaic acid (OA) and structurally related toxins dinophysistoxin-1 (DTX-1), and DTX-2, are lipophilic marine biotoxins. The current reference method for the analysis of these toxins is the mouse bioassay (MBA). This method is under increasing criticism both from an ethical point of view and because of its limited sensitivity and specificity. Alternative replacement methods must be rapid, robust, cost effective, specific and sensitive. Although published immuno-based detection techniques have good sensitivities, they are restricted in their use because of their inability to: (i) detect all of the OA toxins that contribute to contamination; and (ii) factor in the relative toxicities of each contaminant. Monoclonal antibodies (MAbs) were produced to OA and an automated biosensor screening assay developed and compared with ELISA techniques. The screening assay was designed to increase the probability of identifying a MAb capable of detecting all OA toxins. The result was the generation of a unique MAb which not only cross-reacted with both DTX-1 and DTX-2 but had a cross-reactivity profile in buffer that reflected exactly the intrinsic toxic potency of the OA group of toxins. Preliminary matrix studies reflected these results. This antibody is an excellent candidate for the development of a range of functional immunochemical-based detection assays for this group of toxins.

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“…Mais recentemente tem sido empregado o método de varredura e confirmação por ressonância de superfície por plasma ("surface plasmon resonance"), que utiliza biossensores específi-cos, providos com uma camada de cloranfenicol derivativo e um anticorpo que possui alta reatividade com o metabólito cloranfenicol glucoronídeo. Este último método, tem sido comparado com CLAE-EM/EM ou empregado em conjunto 32,33 . A técnica de CLAE-EM/EM vem se destacando devido à sua alta especificidade analítica quando utilizada em modo "Multiple Reaction Monitoring" (MRM), no qual os analisadores de massas Q1 e Q3, selecionam os íons precursor e produto, respectivamente, definindo uma transição de m/z específica.…”

Section: O Composto Cloranfenicol (Cap) D-(-)-treo-22-dicloro-n-unclassified

Determinação de resíduos de cloranfenicol em amostras de leite e mel industrializados utilizando a técnica de espectrometria de massas em "tandem" (CLAE-EM/EM)

et al. 2006

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Detection of chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and milk using a surface plasmon resonance biosensor and Qflex® kit chloramphenicol

Cited by 141 publications

References 7 publications

Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Determinação de resíduos de cloranfenicol em amostras de leite e mel industrializados utilizando a técnica de espectrometria de massas em "tandem" (CLAE-EM/EM)

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