Detection of Chlamydia trachomatis in Clinical Specimens by Nucleic Acid

Hyypiä, Timo; Jalava, Annika; Larsen, Steven H.; Terho, P; Hukkanen, Veijo

doi:10.1099/00221287-131-4-975

Cited by 29 publications

(13 citation statements)

References 5 publications

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“…However, in cases where the infecting species is unknown (suspected C. pneumoniae infection) the genus specific probe, pFEN207 should be used. These sensitivity levels are similar to those which have been reported for other DNA probes using radioisotopic detection methods [8,9] and are comparable with levels obtainable with fluorescent antibody staining [11]. PCR was used to increase the sensitivity of to the amplified product and this further increased the sensitivity.…”

Section: Resultssupporting

confidence: 83%

“…These sensitivity levels are similar to those which have been reported for other DNA probes using radioisotopic detection methods [8,9] and are comparable with levels obtainable with fluorescent antibody staining [11]. However, in cases where the infecting species is unknown (suspected C. pneumoniae infection) the genus specific probe, pFEN207 should be used.…”

Section: Psittaci Infections In Birds or In Humans Withsupporting

confidence: 81%

“…The current study was undertaken to develop sensitive detection methods based on the detection of chlamydial DNA. Most of the work previously reported has been in the development of DNA probes for the detection of C. trachomatis [8,9]. In comparison, little work has been done using DNA probes for the detection of C. psittaci DNA [10,11].…”

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confidence: 99%

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Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction

Rasmussen

1991

FEMS Microbiology Letters

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Fewer than 10(5) elementary bodies of Chlamydia psittaci could be detected by using DNA hybridisation with a plasmid probe specific for avian chlamydial strains. PCR amplification of chlamydial DNA using primers specific for conserved regions of the major outer membrane protein gene enabled the detection of fewer than 10 elementary bodies. DNA could be amplified from 22 of the 24 chlamydial strains tested including avian, feline, ovine, caprine, koala and lymphogranuloma venereum strains.

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Section: Resultssupporting

confidence: 83%

Section: Psittaci Infections In Birds or In Humans Withsupporting

confidence: 81%

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Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction

Rasmussen

1991

FEMS Microbiology Letters

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“…The development of such probes may be impeded by a relative scarcity of ribosomal ribonucleic acid in EBs and by the low number of chlamydial organisms in most clinical specimens. Early studies with the cloned chlamydial common plasmid as a nucleic acid probe suggested that the sensitivity of such methods was below that required for clinical utility (72,128). In contrast to these studies, Pao et al (129) found that C. trachomatis detection by a nucleic acid probe was as sensitive as cell culture.…”

Section: Antigen Detection Methods For C Trachomatis Organismsmentioning

confidence: 89%

Laboratory diagnosis of human chlamydial infections

Barnes

1989

Clin Microbiol Rev

167

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Chlamydia trachomatis is a human pathogen that causes ocular disease (trachoma and inclusion conjunctivitis), genital disease (cervicitis, urethritis, salpingitis, and lymphogranuloma venereum), and respiratory disease (infant pneumonitis). Respiratory chlamydioses also occur with infection by avian strains of C. psittaci or infection by the newly described TWAR agent. Diagnosis of most acute C. trachomatis infections relies on detection of the infecting agent by cell culture, fluorescent antibody, immunoassay, cytopathologic, or nucleic acid hybridization methods. Individual non-culture tests for C. trachomatis are less sensitive and specific than the best chlamydial cell culture system but offer the advantages of reduced technology and simple transport of clinical specimens. Currently available nonculture tests for C. trachomatis perform adequately as screening tests in populations in which the prevalence of infection is greater than 10%. A negative culture or nonculture test for C. trachomatis does not, however, exclude infection. The predictive value of a positive nonculture test may be unsatisfactory when populations of low infection prevalence are tested. Tests that detect antibody responses to chlamydial infection have limited utility in diagnosis of acute chlamydial infection because of the high prevalence of persistent antibody in healthy adults and the cross-reactivity due to infection by the highly prevalent C. trachomatis and TWAR agents. Assays for changes in antibody titer to the chlamydial genus antigen are used for the diagnosis of respiratory chlamydioses. A single serum sample that is negative for chlamydial antibody excludes the diagnosis of lymphogranuloma venereum.

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“…Probes to detect the presence of Chlamydia trachomatis in specimens collected from genital sites have been reported by Hyypia et al (57). Problems with the sensitivity and specificity of the tests were noted, in that 6 of 30 culture-positive specimens failed to react in the assay, while 7 of 30 specimens negative by culture were positive with the probe in the dot blot assay.…”

Section: Probes For Diarrheal Pathogensmentioning

confidence: 99%

Diagnostic deoxyribonucleic acid probes for infectious diseases

Tenover

1988

Clin Microbiol Rev

239

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Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential both to extend the types of pathogens that can be readily identified and to reduce significantly the time associated with the identification of fastidious microorganisms. Over a dozen commercially prepared deoxyribonucleic acid probe tests are now available. This article explores the development of deoxyribonucleic acid probe tests and reviews the sensitivity, specificity, and predictive values of many of the diagnostic probes developed during the last several years. Prospects for newer, more sensitive detection systems for the products of hybridization reactions are also reviewed.

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Detection of Chlamydia trachomatis in Clinical Specimens by Nucleic Acid

Cited by 29 publications

References 5 publications

Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction

Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction

Laboratory diagnosis of human chlamydial infections

Diagnostic deoxyribonucleic acid probes for infectious diseases

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