Detection of Chlamydia trachomatis endocervical infection in asymptomatic and symptomatic women: Comparison of deoxribonucleic acid probe test with tissue culture

Yang, Lisa I.; Panke, Elizabeth S.; Leist, Phyllis A.; Fry, R.J.M.; Lee, Richard F.

doi:10.1016/s0002-9378(12)90782-6

Cited by 19 publications

(3 citation statements)

References 16 publications

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“…PCR has proven to be more sensitive and specific than conventional microbiologic assays. 4,23 The highly sensitive PCR is, therefore, a valuable tool for studying the prevalence of asymptomatic C trachomatis infections since these infections are often associated with low copy numbers of C trachomatis. 11 To facilitate large prevalence studies, our aim was to detect C trachomatis DNA by PCR in crude cell suspensions of cervical scrapes in symptomatic women attending a gynecologic outpatient department and in a randomly selected population of urban slum dwellers.…”

Section: Cytologymentioning

confidence: 99%

Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Singh

Rastogi²,

Garg³

et al. 2002

Acta Cytologica

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Section: Cytologymentioning

confidence: 99%

Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Singh

Rastogi²,

Garg³

et al. 2002

Acta Cytologica

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“…eine Sensitivität von 93% und eine Spezifität von 98% [18]. Ähnlich günstige Nachweisquoten wurden der Untersuchung mit der DNA-Sonde von anderen Autoren attestiert [17,39]; sie ist mittlerweise im diagnostischen Alltag etabliert [5].…”

Section: Mono-und Mehrfachinfektionenunclassified

Erregerspektrum der Urethritis des Mannes

Gall

Beckert

Meier-Ewert³

et al. 1999

Der Hautarzt

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545 male patients with a tentative diagnosis "urethritis" were examined between November 1984 and December 1994 in the Department of Dermatology and Venerology of the Military Hospital in Ulm. The patients, aged from 18 to 58 years (mean age 24.1 years), were examined according to a standardized diagnostic procedure: Smear preparations from the urethra with subsequent gram staining, bacterial cultures for aerobic bacteria, Neisseria gonorrhoeae (cultures and Phadebact gonococcus test), mycoplasma cultures (Mycoplasma hominis (M. hom.); Ureaplasma urealyticum (U. u), and Chlamydia trachomatis using several methods, primarily DIFT (Syva Micro-Trak). Trichomonas vaginalis counts in urine sediment 441 patients (81%) had 4 or more leukocytes per high-power (x1000) field in the gram stained specimens. In these 441 urethritis-patients the following germs could be detected: Trichomonas vagin 3 (1%), N. gonorrhoeae 80 (18%), Mycoplasma 94 (21%) [U. u. 59, M. hom. 24, both 11], C. trachomatis 114 (26%), other pathogenic bacteria 135 (31%). In 114 patients (26%) no bacteria could be identified. A single infection was diagnosed in a total of 242 patients (55%), a double infection was determined in 71 patients (16%) while a triple infection was found in 14 patients (3%). The spectrum determined in the single infection included the following: N. gonorrhoeae 41 (9%), Mycoplasma 45 (10%), C. trachomatis 67 (15%), other pathogenic bacteria 89 (20%) (most frequently found germs were Enterococcus, beta-hemolytic Streptococcus, Escherichia coli, Staph. aureus). In the double infections combinations with aerobic bacteria dominated. In triple infections, mycoplasma were most common. During the investigation period the number of patients with urethritis symptoms declined at a constant rate.

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“…T he use of cell culture systems for the detection of Chlamydia trachomatis is the gold standard. 1 However, the sensitivity of a single endocervical specimen may be only 70-90. [2][3] Unfortunately, cell culture is labor intensive and requires 48 hours for completion.…”

mentioning

confidence: 99%

The Potential Misuse of DNA Probe for the Detection of Neisseria gonorrhoeae and Chlamydía trachomatis When Used for Test of Cure

Monif¹

1997

Infectious Diseases in Obstetrics and Gynecology

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Detection of Chlamydia trachomatis endocervical infection in asymptomatic and symptomatic women: Comparison of deoxribonucleic acid probe test with tissue culture

Cited by 19 publications

References 16 publications

Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Erregerspektrum der Urethritis des Mannes

The Potential Misuse of DNA Probe for the Detection of Neisseria gonorrhoeae and Chlamydía trachomatis When Used for Test of Cure

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