Detection of Chlamydia trachomatis endocervical infection in asymptomatic and symptomatic women: Comparison of deoxribonucleic acid probe test with tissue culture

Yang, Lisa I.; Panke, Elizabeth S.; Leist, Phyllis A.; Fry, R.J.M.; Lee, Richard F.

doi:10.1016/0002-9378(91)90388-8

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Screening for chlamydia is recommended because 56% to 80% of persons with infections do not experience symptoms. [10][11][12] During the past decade, more sensitive and specific screening tests that are technically easier to perform have become available. 13 Effective antimicrobial drug therapy is available, with cure rates of 97% for a 7-day course of doxycyline costing $2 to $8.…”

Section: From the Department Of Pediatrics University Of Californiamentioning

confidence: 99%

Screening for Chlamydia in Adolescents and Young Women

Mangione-Smith

McGlynn

Hiatt

2000

Arch Pediatr Adolesc Med

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To measure the proportion of sexually active females aged 15 to 25 years who received a screening test for Chlamydia trachomatis infection during the previous year. Design: Administrative data were used to identify females in the target age range who were likely to be sexually active. Medical record data were reviewed for a sample to determine whether the administrative algorithm was acceptable. Laboratory claims data and medical record data were used to identify females who had had a screening test for chlamydia. Setting: Four geographically dispersed US managed health care plans. Patients: We studied 19214 sexually active females aged 15 to 25 years continuously enrolled for calendar year 1997 in 1 of 4 major US health plans who had a visit to their health care provider during that year. Sexual activity was determined using an algorithm designed for use with administrative data. Main Outcome Measure: Rates of chlamydia screening among sexually active females aged 15 to 25 years. Results: The proportion of females aged 15 to 25 years identified as sexually active by the administrative data algorithm in the 4 health plans was similar (43%-54%; P=.79). However, substantial variation was found in rates of chlamydia screening for eligible females in these 4 health plans (2%-42%; PϽ.001). Plans varied considerably in the types of visits (eg, sexually transmitted disease screening or pregnancy) that determined eligibility for the measure. Conclusions: A measure of health plan performance on screening for chlamydia in young females using administrative data is feasible and provides useful results despite some flaws in estimation. There is room for improvement in rates of chlamydia screening in sexually active females aged 15 to 25 years.

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Section: From the Department Of Pediatrics University Of Californiamentioning

confidence: 99%

Screening for Chlamydia in Adolescents and Young Women

Mangione-Smith

McGlynn

Hiatt

2000

Arch Pediatr Adolesc Med

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“…A previous study, which used isolation in Mc-Coy cells in 96-well microtiter plates with subpassage as the reference method, showed a sensitivity of 89% and a specificity of 96% for the MicroTrak EIA with endocervical specimens obtained from a high-prevalence (14%) female population (9). In five studies comparing PACE 2 with culture, the sensitivities for the probe assay ranged from 86.1 to 93% and the specificities ranged from 97.7 to 100% (12, [14][15][16]28). Several factors, including patient characteristics and infection prevalence (2,8,13,17,27) as well as differences in specimen collection (19) and culture techniques (1,13,25,27,29), may affect test performance.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Syva MicroTrak enzyme immunoassay and Gen-Probe PACE 2 with cell culture for diagnosis of cervical Chlamydia trachomatis infection in a high-prevalence female population

et al. 1993

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Culture is currently considered the "gold standard" for detecting Chiamydia trachomatis infections. We evaluated the Syva MicroTrak enzyme immunoassay (EIA) and Gen-Probe PACE 2 tests, which detect chlamydial antigens and rRNA, respectively. These assays were compared with each other and with culture for the detection of C. trachomatis in cervical specimens obtained from 217 women attending a clinic for sexually transmitted diseases. The prevalence of infection was 22.1% by culture. The sensitivity, specificity, and positive and negative predictive values were 79.2, 98.2, 92.6, and 94.3%, respectively, for EIA. For PACE 2, the respective values were 77.1, 97.6, 90.1, and 93.7%. After corrections for two false-negative cultures, the sensitivities and specificities were 80 and 99.4%, respectively, for the EIA and 78 and 98.8%, respectively, for the probe assay. Quantitative evaluation of the results showed that false-negative results with either assay were associated with cultures that had low inclusion counts or were negative without subpassage. Analysis of nonculture results revealed that 2.3% of the EIA results and 4.6% of the probe assay results were within ±30%o of the respective assay cutoff values. These included four false-negative (one EIA and three probe) and two false-positive (one EIA and one probe) results. The Syva MicroTrak EIA and the Gen-Probe PACE 2 assay are comparable to but significantly less sensitive than culture. Use of a grey zone may help identify the need for repeat or confirmatory testing.

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“…Die Sensitivit~it der Zellkultur betr~igt jedoch nicht 100%, sondern wird in der Literatur zwischen 80 und 90% eingesch~itzt (6,5,7,9). Die Sensitivit~it der Zellkultur betr~igt jedoch nicht 100%, sondern wird in der Literatur zwischen 80 und 90% eingesch~itzt (6,5,7,9).…”

Section: Diskussionunclassified

“…Die Sensifivit~it des Gensondentests wird in anderen Studien zwischen 87 und 93% eingesch~itzt, die Spezifikation zwischen 97 und 100% (4, 5, 8,9). 2).…”

Section: Diskussionunclassified

Stellenwert des Gensondentests in der Routinediagnostik von Chlamydieninfektionen

Klocke¹,

Neuer²

1993

Arch Gynecol Obstet

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ZusammenfassungEin Gensondentest zum Nachweis von Chlamydia trachomatis (PACE R 2, Genprobe, San Diege) wurde mit der Zellkultur als Standardmethode verglichen. Abstriche yon 344 symtomatischen und asymptomatischen Patientinnen, 183 Abstriche yon Cervix und Urethra und 161 Fimbrienabstriche, wurden untersucht Alle Abstrich, in denen in der Zellkultur Chlamydien nachgewiesen werden konnten, reagierten auch im Gensondentest positiv. Von den 20 Abstrichen, die im Gensondentest positiv reagierten, fie1 die Zellkultur jedoch nur in 9 F~illen positiv ans. Bezogen auf die Zellkultur als Standardmethode ergab sich eine Sensitivit~t yon 100% und eine Spezifitat yon 97,1%.

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Detection of Chlamydia trachomatis endocervical infection in asymptomatic and symptomatic women: Comparison of deoxribonucleic acid probe test with tissue culture

Cited by 5 publications

References 15 publications

Screening for Chlamydia in Adolescents and Young Women

Screening for Chlamydia in Adolescents and Young Women

Comparison of the Syva MicroTrak enzyme immunoassay and Gen-Probe PACE 2 with cell culture for diagnosis of cervical Chlamydia trachomatis infection in a high-prevalence female population

Stellenwert des Gensondentests in der Routinediagnostik von Chlamydieninfektionen

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