Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Rectal and Oropharyngeal Swabs and Urine Specimens from Men Who Have Sex With Men with Abbott's M2000 RealTime

Moncada, Jeanne; Shayevich, Clara; Philip, Susan; Lucic, Danijela; Schachter, Julius

doi:10.1097/olq.0000000000000360

Cited by 11 publications

(12 citation statements)

References 3 publications

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“…Despite these limitations, the assay used to detect chlamydial DNA is widely used to detect genital infections. Previous studies have shown RealTime to be highly sensitive (95.3%) and specific (99.9%) for the diagnosis of C. trachomatis from urine and ocular swabs [ 13 , 28 , 29 ]. The RealTime assay has compared favorably to other nucleic acid amplification tests for detecting C. trachomatis DNA and RNA commonly used in trachoma research [ 13 , 28 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Ocular Chlamydia trachomatis Infection Under the Surgery, Antibiotics, Facial Cleanliness, and Environmental Improvement Strategy in Amhara, Ethiopia, 2011–2015

Nash

Stewart

Zerihun

et al. 2018

Clinical Infectious Diseases

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BackgroundWorld Health Organization (WHO) recommendations for starting and stopping mass antibiotic distributions are based on a clinical sign of trachoma, which is indirectly related to actual infection with the causative agent, Chlamydia trachomatis.MethodsThis study aimed to understand the effect of SAFE (surgery, antibiotics, facial cleanliness, and environmental improvement) interventions on ocular chlamydia in Amhara, Ethiopia, by describing the infection prevalence in a population-based sample of children aged 1–5 years. Trachoma surveys were conducted in all districts of Amhara, from 2011 to 2015 following approximately 5 years of SAFE. Ocular swabs were collected from randomly selected children to estimate the zonal prevalence of chlamydial infection. The Abbott RealTime polymerase chain reaction assay was used to detect C. trachomatis DNA.ResultsA total of 15632 samples were collected across 10 zones of Amhara. The prevalence of chlamydial infection in children aged 1–5 years was 5.7% (95% confidence interval, 4.2%–7.3%; zonal range, 1.0%–18.5%). Chlamydial infection and trachomatous inflammation–intense (TI) among children aged 1–9 years were highly correlated at the zonal level (Spearman correlation [r] = 0.93; P < .001), while chlamydial infection and trachomatous inflammation–follicular were moderately correlated (r = 0.57; P = .084).ConclusionsAfter 5 years of SAFE, there is appreciable chlamydial infection in children aged 1–5 years, indicating that transmission has not been interrupted and that interventions should continue. The sign TI was highly correlated with chlamydial infection and can be used as a proxy indicator of infection.

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Section: Discussionmentioning

confidence: 99%

Ocular Chlamydia trachomatis Infection Under the Surgery, Antibiotics, Facial Cleanliness, and Environmental Improvement Strategy in Amhara, Ethiopia, 2011–2015

Nash

Stewart

Zerihun

et al. 2018

Clinical Infectious Diseases

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“…77–79 Testing of rectal and oropharyngeal specimens should be routine in MSM, considered in women who are sexual contacts of gonorrhoea patients [1C], and be guided based on sexual history, risk and symptoms or signs in all other patients. 3,39,80–89 Testing of pooled specimens (oropharyngeal, rectal, and urine/urogenital) could provide cost savings, but is not recommended, due to potentially decreased sensitivity, 3,39,90–93 increased complexity, including risk of cross-contamination of sample, and lack of approval by FDA or other regulatory agencies. However, the evidence is limited and additional appropriate studies using different NAATs and evaluating cost and benefits (e.g.…”

Section: Testing and Diagnosismentioning

confidence: 99%

“…Testing of rectal and oropharyngeal specimens should be routine in MSM, considered in women who are sexual contacts of gonorrhoea patients [1C], and be guided based on sexual history, risk and symptoms or signs in all other patients. 3,39,80–89…”

Section: Testing and Diagnosismentioning

confidence: 99%

Background review for the ‘2020 European guideline for the diagnosis and treatment of gonorrhoea in adults’

et al. 2020

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Gonorrhoea is a major public health concern globally. Increasing incidence and sporadic ceftriaxone-resistant cases, including treatment failures, are growing concerns. The 2020 European gonorrhoea guideline provides up-to-date evidence-based guidance regarding the diagnosis and treatment of gonorrhoea. The updates and recommendations emphasize significantly increasing gonorrhoea incidence; broad indications for increased testing with validated and quality-assured nucleic acid amplification tests (NAATs) and culture; dual antimicrobial therapy including high-dose ceftriaxone and azithromycin (ceftriaxone 1 g plus azithromycin 2 g) OR ceftriaxone 1 g monotherapy (ONLY in well-controlled settings, see guideline for details) for uncomplicated gonorrhoea when the antimicrobial susceptibility is unknown; recommendation of test of cure (TOC) in all gonorrhoea cases to ensure eradication of infection and identify resistance; and enhanced surveillance of treatment failures when recommended treatment regimens have been used. Improvements in access to appropriate testing, test performance, diagnostics, antimicrobial susceptibility surveillance and treatment, and follow-up of gonorrhoea patients are essential in controlling gonorrhoea and to mitigate the emergence and/or spread of ceftriaxone resistance and multidrug-resistant and extensively drug-resistant gonorrhoea. This review provides the detailed background, evidence base and discussions, for the 2020 European guideline for the diagnosis and treatment of gonorrhoea in adults (Unemo M, et al. Int J STD AIDS. 2020).

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“…For positive individual samples, the m2000 generated delta cycle threshold result was converted to Ct elementary body (EB) equivalent concentration based on a previously described calibration curve of known EB concentrations on the RealTime Assay. 27,28 Once Ct load was known for the positive individual samples, a total of 240 with the highest load were chosen for this project. Samples with sufficient Ct load, likely to obtain high quality full genome sequence data based on our previous studies, were re-extracted as described below.…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Genomics of OcularChlamydia trachomatisafter 5 years of SAFE interventions for trachoma in Amhara, Ethiopia

Pickering

Chernet

Sata

et al. 2020

Preprint

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BackgroundTo eliminate trachoma as a public health problem, the WHO recommends the SAFE strategy. As part of the SAFE strategy in the Amhara Region, Ethiopia, the Trachoma Control Program distributed over 124 million doses of antibiotic between 2007 and 2015. Despite these interventions, trachoma remained hyperendemic in many districts and a considerable level of Chlamydia trachomatis (Ct) infection was evident. Methods

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Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Rectal and Oropharyngeal Swabs and Urine Specimens from Men Who Have Sex With Men with Abbott's M2000 RealTime

Cited by 11 publications

References 3 publications

Ocular Chlamydia trachomatis Infection Under the Surgery, Antibiotics, Facial Cleanliness, and Environmental Improvement Strategy in Amhara, Ethiopia, 2011–2015

Ocular Chlamydia trachomatis Infection Under the Surgery, Antibiotics, Facial Cleanliness, and Environmental Improvement Strategy in Amhara, Ethiopia, 2011–2015

Background review for the ‘2020 European guideline for the diagnosis and treatment of gonorrhoea in adults’

Genomics of OcularChlamydia trachomatisafter 5 years of SAFE interventions for trachoma in Amhara, Ethiopia

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