Detection of Chlamydia pneumoniae DNA in Buffy-Coat Samples of Patients with Abdominal Aortic Aneurysm

Maraha, B.; Heijer, Martin den; Wullink, M.; Zee, Anneke van der; Bergmans, Anneke; Verbakel, Harold; Kerver, Marjolein; Graafsma, S.J.; Kranendonk, Steef; Peeters, M.P.D.

doi:10.1007/pl00011240

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…Previous studies reported different prevalence of CP by PCR in specimens obtained from abdominal aortic aneurysm, ranging from 20% to 49%. 4,5,6,7 Therefore our positivity rates are within the expected range. In our study, an optimized DNA extraction protocol to reduce any underestimation of CP prevalence was used, and our data were confirmed by nonradioactive DNA hybridization to enhance specificity.…”

Section: Discussionsupporting

confidence: 74%

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

et al. 2002

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Background-Only a few studies have focused the attention on the relation between elevated anti-Chlamydia pneumoniae (CP) antibodies and the detection of CP in the arterial wall. The aim of our study is thus to investigate the relationship between immune response to CP and detection of CP in the aortic walls of patients with abdominal aortic aneurysm. Methods and Results-A specimen of aortic wall was obtained from 102 consecutive patients who underwent abdominal aneurysm repair. The possible presence of CP was studied by polymerase chain reaction and confirmed by nonradioactive DNA hybridization. Antibody response to CP was studied (IgG, IgA titers). We found 33 patients (32.4%) with CP DNAϩ. No correlation between CP DNA detection and antibody titers was found (IgG Pϭ0.52, IgA Pϭ0.66). High correlation between IgG and IgA titer was observed (PϽ0.01). Endovascular presence of CP and antibody titers was not related to the age of the patient. Conclusions-CP antibody titers are not associated with the presence of CP in the aortic wall of patients with abdominal aortic aneurysm.

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Section: Discussionsupporting

confidence: 74%

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

et al. 2002

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“…A relatively large study found C. pneumoniae DNA in PBMC associated with CAD in men (8.8% positive patients versus 2.9% controls (OR, 3.2) [28]. Association with abdominal aortic aneurysm (OR, 2.6 [1.1-6.3]) was also shown in a cross-sectional study [26]. Further validation in larger populations with a standardized PCR technique is needed.…”

Section: Evidence Of Association With Cardiovascular and Cerebrovascumentioning

confidence: 89%

“…Moreover, CAD and acute coronary events, which have mostly been studied, are not a good measure of the severity and extent of atherosclerosis throughout the body, and they do not provide information on initiation or acceleration of the disease process, but rather on precipitation of acute ischemia only. Measuring C. pneumoniae DNA PBMCs has been suggested as a better marker of chronic infection [19,23,25,26]; correlation with the presence of the organism in atheromatous plaque was good in one study [27], but poor in another [24]. A relatively large study found C. pneumoniae DNA in PBMC associated with CAD in men (8.8% positive patients versus 2.9% controls (OR, 3.2) [28].…”

Section: Evidence Of Association With Cardiovascular and Cerebrovascumentioning

confidence: 95%

Chlamydia pneumoniae and cardiovascular disease

Kolia

Fong

2002

Curr Infect Dis Rep

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Chlamydia pneumoniae, a respiratory pathogen, has been suggested as a risk factor for cardiovascular disease. Epidemiologic data are very controversial. Histopathologic and microbiologic studies have established an association between atherosclerosis and presence of C. pneumoniae, consistently finding C. pneumoniae DNA and antigens in atherosclerotic arteries. C. pneumoniae has been cultured from atherosclerotic arteries in several centers. An etiologic role for C. pneumoniae in initiation, acceleration of atherosclerosis, and/or acute ischemia remains debatable. In vitro studies have shown that C. pneumoniae can induce foam cell formation, low-density lipoprotein oxidation, and proinflammatory and procoagulant cytokine expression. Animal models of de novo initiation or enhancement of atherosclerosis have been developed. Preliminary trials of secondary prevention of coronary artery disease complications by antimicrobial agents show modest results. Better diagnostic tools, more diverse animal models, and clinical trials of primary prevention are needed. Meanwhile, results of ongoing large clinical trials on secondary prevention are eagerly awaited, but may not be definitive.

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“…The methods by which interview data were obtained and blood samples were collected have been described elsewhere. 19 In brief, all participants completed a questionnaire about medical history and medication use. Peripheral vascular disease was defined either as a history of claudicatio intermittens 20 or when a person had undergone surgery because of claudicatio intermittens.…”

Section: Study Population and Specimen Collectionmentioning

confidence: 99%

Abdominal Aortic Aneurysm Is Associated With High Serum Levels of Tenascin-X and Decreased Aneurysmal Tissue Tenascin-X

et al. 2006

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Background-Tenascin-X is a large extracellular matrix protein that is abundantly expressed in several connective tissues.A 140-kDa C-terminal fragment of tenascin-X is present in human serum. Complete deficiency of tenascin-X is associated with Ehlers-Danlos syndrome, and these patients show major connective tissue alterations in their skin, as well as blood vessel fragility. In this study, we investigated whether tenascin-X is present in normal human aorta and abdominal aortic aneurysm (AAA) tissues and whether an association exists between serum tenascin-X levels and AAA. Methods and Results-Five normal aortas and 5 AAA tissues were immunostained for tenascin-X and elastin. Tenascin-X was present throughout the entire aorta and was especially abundant near the elastic lamellae, whereas tenascin-X expression was strongly decreased in AAA tissue. Measurement of tenascin-X serum concentration by enzyme-linked immunosorbent assay (ELISA) in 87 AAA patients and 86 controls demonstrated an increasing risk for AAA with increasing tenascin-X serum concentrations. After adjustment for established risk factors, tenascin-X serum concentrations in the highest quartile were associated with a 5-fold increase in risk of AAA (odds ratio, 5.3; 95% confidence interval, 2.0 to 13.8). Conclusions-Tenascin

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Detection of Chlamydia pneumoniae DNA in Buffy-Coat Samples of Patients with Abdominal Aortic Aneurysm

Cited by 15 publications

References 35 publications

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

Chlamydia pneumoniae and cardiovascular disease

Abdominal Aortic Aneurysm Is Associated With High Serum Levels of Tenascin-X and Decreased Aneurysmal Tissue Tenascin-X

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