Detection of chicken anaemia virus by DNA hybridization and polymerase chain reaction

Noteborn, Mathieu H. M.; Verschueren, C. A. J.; Roozelaar, D J van; Veldkamp, S.; Eb, A. J. Van Der; Boer, G. F. de

doi:10.1080/03079459208418823

Cited by 41 publications

(14 citation statements)

References 17 publications

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“…Expression of apoptin for 5 days resulted in fragmentation of the nuclei into apoptotic bodies ( Figure 3a). Apoptin expression also induced apoptosis in HeLa cervical carcinoma cells which have previously been reported to be resistant to apoptosis induced by apoptin (Noteborn et al, 1994). At 5 days posttransfection virtually all HeLa cells expressing apoptin showed clear signs of apoptosis.…”

Section: Apoptin Translocates To the Nucleus And Induces Apoptosismentioning

confidence: 75%

“…The subcellular localization of apoptin may be important for induction of cell death. In cells resistant to apoptin-mediated cell death the protein had a cytoplasmic localization, whereas in sensitive cells it was found in the nucleus (Noteborn et al, 1994;Zhuang et al, 1995a-c;DanenVan Oorschot et al, 1997) Recently, apoptin was described to be phosphorylated specifically in tumour and transformed cells both in vitro and in vivo. This modification was shown to be important for the nuclear localization and cell killing ability of apoptin (Rohn et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Apoptin is a 13.6 kDa, serine and threonine rich protein which consists of 121 amino acids (aa) containing two positively charged and two proline rich domains (Noteborn et al, 1991(Noteborn et al, , 1994. Apoptin-induced apoptosis occurs independently of the tumour-suppressor gene p53 (Zhuang et al, 1995c).…”

Section: Introductionmentioning

confidence: 99%

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TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells

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Section: Apoptin Translocates To the Nucleus And Induces Apoptosismentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells

Guelen¹,

Paterson

Gäken³

et al. 2003

Oncogene

125

148

View full text Add to dashboard Cite

“…Infection with CAV causes a disease in young chickens that is characterized by a generalized lymphoid atrophy, severe anemia and increased mortality. The death of infected cells is caused by apoptin (VP3), a small 14 kDa virally encoded and proline-rich protein, which has no homologous cellular counterparts (Noteborn et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Apoptin-induced cell death is modulated by Bcl-2 family members and is Apaf-1 dependent

et al. 2005

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“…Later a study proposed a possible second serotype (Spackman, et al, 2002a,b). However, studies have been carried out to characterize the virus based on its DNA sequence (Noteborn et al, 1992;Todd et al, 1992;Tham and Stanislawek, 1992). Both Southern analysis and restriction mapping showed only minor differences on the CAV genomes of the field isolates from United States (Noteborn et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

et al. 2018

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Four DNA fragments (fragments A, B, C and D) covering the whole genome of chicken anemia virus (CAV) were amplified enzymatically by polymerase chain reaction (PCR) using four pairs of oligonucleotide primers. The DNA fragments were amplified from each of nine CAV isolates including eight Malaysian isolates and one European isolate (Cux-1). For all nine CAV isolates, fragment A (1518 bp) was digested with one restriction enzyme, Eco130I (StyI); fragment B (926 bp) with three enzymes, Eco130I (StyI), HpaII and MboI separately; fragment C (675 bp) with also three enzymes, BsuRI (HaeIII), HinfI, and HpaII separately; and the fragment D (552 bp) with one enzyme, EcoRI. Enzyme digested products of different fragments were separated by agarose gel or polyacrylamide gel electrophoresis. Each of the eightenzymatic reactions differentiated at least two isolates except the HpaII digestion of fragment C where no isolate was distinguished. The overall restriction endonuclease (RE) analysis separated four isolates (BL-1, BL-2, BL-4 and BL-5) in one group and the rest five isolates (SMSC-1, SMSC-2, 3-1, BL-3 and Cux-1) were differentiated from each other and also from the group of four isolates, based on the number of restriction site differences and the fragments generated by different enzymatic digestions. The study revealed that RE analysis could be used to identify and differentiate CAV isolates based on the number of restriction site differences. The study showed that more isolates, even the isolates from the same poultry farm, could be differentiated with proper genomic diversity after RE analysis of more genome fragments compared to that of single genome fragment.

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Detection of chicken anaemia virus by DNA hybridization and polymerase chain reaction

Cited by 41 publications

References 17 publications

TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells

TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells

Apoptin-induced cell death is modulated by Bcl-2 family members and is Apaf-1 dependent

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

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