Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor

Higashiyama, Takumi; Katsuyama, Akihiro; Otori, Hideki; Kamimura, Toru; Uehara, Atsushi; Kainuma, Miho; Takumi, Ryo; Kudo, Yukako; Ebina, Masayuki; Mochitate, Katsumi; Kon, T.; Furuya, Yasubumi; Kikuchi, Hideaki

doi:10.1016/j.ultras.2014.04.026

Cited by 11 publications

(8 citation statements)

References 22 publications

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“…Our observation is in line with previous literary knowledge about the oxidative stress-induced structural and functional modification of intracellular macromolecules, including filamentous actin resulting in its depolymerisation and thereby the disruption of the physiological cytoskeleton [ 39 ]. Actin filaments are associated with the intracellular localisation of CAMs, including cadherins, tight-junction, and focal adhesion proteins [ 40 , 41 ], ensuring the stable cell surface and cell-cell adhesion and thereby the maintenance of the mucosal barrier function [ 42 , 43 ]. Interestingly, we also observed that the Comp23 treatment increased the expression of CAMs and, more importantly, preserved the healthy architecture of the actin cytoskeleton-CAM complexes in the epithelial cells exposed to oxidative stress (Figures 4 and 5 ).…”

Section: Discussionmentioning

confidence: 99%

PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease

Veres-Székely

Bernáth

Pap³

et al. 2020

Oxidative Medicine and Cellular Longevity

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Coeliac disease (CD) is a chronic, immune-mediated small intestinal enteropathy, accompanied with gluten-triggered oxidative damage of duodenal mucosa. Previously, our research group reported an increased mucosal level of the antioxidant protein Parkinson’s disease 7 (PARK7) in children with CD. In the present study, we investigated the role of increased PARK7 level on the epithelial cell and mucosal integrity of the small intestine. The presence of PARK7 was investigated using immunofluorescent staining on duodenal mucosa of children with CD and on FHs74Int duodenal epithelial cells. To investigate the role of oxidative stress, FHs74Int cells were treated with H2O2 in the absence or presence of Comp23, a PARK7-binding compound. Intracellular accumulation of reactive oxygen species (ROS) was determined by DCFDA-based assay. Cell viability was measured by MTT, LDH, and Annexin V apoptosis assays. Disruption of cytoskeleton and cell adhesion was investigated by immunofluorescence staining and by real-time RT PCR. Effect of PARK7 on mucosal permeability was investigated ex vivo using intestinal sacs derived from control and Comp-23-pretreated mice. Comp23 treatment reduced the H2O2-induced intracellular accumulation of ROS, thus preserving the integrity of the cytoskeleton and also the viability of the FHs74Int cells. Accordingly, Comp23 treatment increased the expression of antioxidants (NRF2, TRX1, GCLC, HMOX1, NQO1), cell-cycle regulators (TP53, CDKN1A, PCNA, BCL2, BAX), and cell adhesion molecules (ZO1, CDH1, VCL, ITGB5) of H2O2-treated cells. Pretreatment with Comp23 considerably decreased the small intestinal permeability. In this study, we demonstrate that PARK7-binding Comp23 reduces the oxidative damage of duodenal epithelial cells, via increased expression of NRF2- and P53-regulated genes. Our results suggest that PARK7 plays a significant role in the maintenance of mucosal integrity in CD.

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Section: Discussionmentioning

confidence: 99%

PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease

Veres-Székely

Bernáth

Pap³

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Thus, the SAW sensor achieved the ability to analyse the extent of wound healing without microscopic observation 95 . The effect of H2O2 (0.5 and 1.0 mM) on cellular tissue function, particularly transepithelial resistance and paracellular permeability, under the same conditions was investigated by attaching cultured cells to a cell adhesion matrix of a SH-SAW device, and the loss and phase shift of the SH-SAW signal was observed in the open channe 96 . The 2014 Ebola outbreak was the largest in history, and early detection of this highly infectious virus will help improve infection control measures.…”

Section: Detection Of Cellsmentioning

confidence: 99%

“…95 The effect of H 2 O 2 (0.5 and 1.0 mM) on cellular tissue function, particularly transepithelial resistance and paracellular permeability, under the same conditions was investigated by attaching cultured cells to a cell adhesion matrix of the SH-SAW device, and the loss and phase shift of the SH-SAW signal was observed in the open channel. 96 The 2014 Ebola outbreak was the largest in history, and the early detection of this highly infectious virus will help improve infection control measures. The SH-SAW platform could obtain phase shifts by continuously measuring the difference between the reference signal and the Ebola signal and could detect target antigens in various types of media, reaching detection limits of <50 pg cm −2 , with fast virus detection (within 2 min) (Fig.…”

Section: Detection Of Cellsmentioning

confidence: 99%

Surface acoustic wave based microfluidic devices for biological applications

et al. 2023

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“…These thin films with various SAW properties are appropriate for diverse applications. For example, shear-horizontal (SH) SAW, which can be converted to Love-mode wave [13,14], is suitable for sensing in liquid environments [15,16]. A-plane ZnO (a-ZnO) can be deposited on r-plane sapphire (r-sapphire) substrate, in which Love-mode SAW can be excited [17].…”

Section: Introductionmentioning

confidence: 99%

Systematical Study of the Basic Properties of Surface Acoustic Wave Devices Based on ZnO and GaN Multilayers

Shen

et al. 2020

Electronics

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Recently, surface acoustic wave (SAW) devices based on layered structures are a popular area of research. Multilayered structures, including ZnO and GaN, have shown great performance and can be applied in diverse fields. Meanwhile, thin films, such as AlGaN and n-ZnO, can be added to these structures to form a 2-D electron gas (2DEG) which makes the devices tunable. This work systematically studies the basic properties of SAW devices based on ZnO and GaN multilayers via COMSOL Multiphysics. The sorts of structures with different crystal orientations are simulated, and various acoustic modes are considered. Results show that a range of phase velocity from about 2700 m/s to 6500 m/s can be achieved, and devices based on ZnO and GaN multilayers can meet the requirements of the electromechanical coupling coefficient from about 0 to 7%. Every structure’s unique properties are valuable for diverse applications. For example, c-ZnO/c-GaN/c-sapphire structure can be used for high-frequency and large-bandwidth SAW devices, while SAW devices based on a-ZnO/a-GaN/r-sapphire and 2DEG are suitable for programmable SAW sensors. This work has great reference value for future research into SAW devices.

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Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor

Cited by 11 publications

References 22 publications

PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease

PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease

Surface acoustic wave based microfluidic devices for biological applications

Systematical Study of the Basic Properties of Surface Acoustic Wave Devices Based on ZnO and GaN Multilayers

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