Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test

Rao, Morubagal Raghavendra; Chandrashaker, Pooja; Mahale, Rashmi P; Shivappa, Sowmya Govindanahalli; Gowda, Ranjitha Shankare; Chitharagi, Vidyavathi B

doi:10.4103/jlp.jlp_132_18

Cited by 10 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different phenotypic approaches are employed for the diagnosis of CPE, including the modified Hodge test (MHT), Blue-Carba test and Rapidec CARBA NP test. The Rapidec CARBA NP test is a biochemical assay with a good sensitivity and specificity, but it cannot differentiate among types of carbapenemases [ 7 , 11 ]. This test is also recommended by the Clinical and Laboratory Standards Institute (CLSI) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

NDM Production as a Dominant Feature in Carbapenem-Resistant Enterobacteriaceae Isolates from a Tertiary Care Hospital

et al. 2021

View full text Add to dashboard Cite

The worldwide spread and increasing prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is of utmost concern and a problem for public health. This resistance is mainly conferred by carbapenemase production. Such strains are a potential source of outbreaks in healthcare settings and are associated with high rates of morbidity and mortality. In this study, we aimed to determine the dominance of NDM-producing Enterobacteriaceae at a teaching hospital in Karachi. A total of 238 Enterobacteriaceae isolates were collected from patients admitted to Jinnah Postgraduate Medical Centre (Unit 4) in Karachi, Pakistan, a tertiary care hospital. Phenotypic and genotypic methods were used for detection of metallo-β-lactamase. Out of 238 isolates, 52 (21.8%) were CRE and 50 isolates were carbapenemase producers, as determined by the CARBA NP test; two isolates were found negative for carbapenemase production by CARB NP and PCR. Four carbapenemase-producing isolates phenotypically appeared negative for metallo-β-lactamase (MBL). Of the 52 CRE isolates, 46 (88.46%) were blaNDM positive. Most of the NDM producers were Klebsiella pneumoniae, followed by Enterobacter cloacae and Escherichia coli. In all the NDM-positive isolates, the blaNDM gene was found on plasmid. These isolates were found negative for the VIM and IPM MBLs. All the CRE and carbapenem-sensitive isolates were sensitive to colistin. It is concluded that the NDM is the main resistance mechanism against carbapenems and is dominant in this region.

show abstract

Section: Introductionmentioning

confidence: 99%

NDM Production as a Dominant Feature in Carbapenem-Resistant Enterobacteriaceae Isolates from a Tertiary Care Hospital

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The dread of extended spectrum β-lactamase (ESβL) enzyme to get new ability for developing antimicrobial resistance and multidrug-resistant (MDR) mechanisms, in Morganella morganii which was formed about 4.1% of whole wound infections and Pseudomonas aeruginosa which is causative agent for post-surgery wound infections and is considered as an important pathogen of both community and hospital acquired infections, and the ability to cause infections as Carbapenemase and MDR agents, also could be important source of concern since these organisms are not only resistant to betalactam antibiotics but also show cross-resistance to other groups of antibiotics [8,10,20] (Table 4…”

Section: Discussionmentioning

confidence: 99%

Multidrug Resistant Bacterial Profile and Patterns for Pus Isolates and Recurrent Wound Infections in Nongovernmental Hospitals of Jordan

Abu-Harirah

Amawi

Deeb

et al. 2021

JPRI

View full text Add to dashboard Cite

Background: Many types of infection can cause pus Infections involving the bacteria; E.coli, so the assessment of multidrug Bacterial profile and patterns is needed to understand the source and management of these injuries. Purpose: To determine infections and patterns toward antibiotics of pus isolates and recurrent wound infections in nongovernmental hospitals of Jordan Methods: During period eleven months, 607 Patients were involved, out of which 128 patients had pus samples and/or recurrent wound infections. Data analysis was done using SPSS version 20. P value was set at <0.05. Results: One hundred twenty eight (21.1%) out of 607 patients were identified to had pus isolates and/or wound recurrent infections 86(87%) out of 128 patients had infections with known pathogenic microbes. Microbiological culture pattern was total of 19 different pathogenic microorganisms were isolated from the participants, with mixed gram-positive and gram-negative species; percentage of 37% gram-positive aerobic bacteria and 63% gram-negative aerobic bacteria. Conclusion: The global burden from multidrug resistant bacteria highly impacted in wound and pus-causing infections, either in hospital acquired infections or community acquired infections. The main causative agents of recurrent wound infection were Staph. aureus MRSA, E. coli, Pseudomonas aeruginosa, Acinetobacter spp (MDR). Gram-negative bacteria caused the most of infections by more than 67% comparing with gram-positive bacteria.

show abstract

“…To establish carbapenemase-production in the isolates included in the study, the isolates were subjected to the modified Hodge test (MHT) and the modified carbapenem-inactivation method (mCIM), optimized for P. aeruginosa, as previously described (Chou et al 2020;Pitout et al 2008;Rao et al 2019). In both assays, MER disks (10 µg; Oxoid, Basingstoke, UK) were utilized and Escherichia coli ATCC 25922 was used as an indicator organism.…”

Section: Phenotypic Detection Of Carbapenemase and Metallo-βlactamase (Mbl) Productionmentioning

confidence: 99%

Insights on carbapenem-resistant Pseudomonas aeruginosa

et al. 2021

View full text Add to dashboard Cite

Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is a signiﬁcant clinical concern. In this study, phenotypic methods were used to characterize ﬁfty-four (n = 54) P. aeruginosa isolates, which were included based on their suspected non-susceptibility to meropenem. Minimum inhibitory concentrations (MICs) of meropenem, ceftazidime, cefepime, ciprofloxacin, gentamicin, were determined using E-tests, while colistin MICs were determined using broth microdilution. The isolates were subjected to the modiﬁed Hodge test (MHT), the modiﬁed carbapenem-inactivation method (mCIM) and the imipenem/EDTA combined disk test (CDT). AmpC and eﬄux pump overexpression was studied using agar plates containing cloxacillin and phenylalanine-arginine β-naphthylamide (PAβN), respectively. Assessment of bioﬁlm-formation was carried out using the crystal violet tube-adherence method. 38.9% of the strains showed meropenem MICs in the resistant range (>8 mg/L). Eﬄux-pump overexpression and AmpC-hyperproduction was seen in 44.4% and 35.2% of isolates, respectively. 88.8% of the isolates were characterized as strong bioﬁlm-producers. On the other hand, the presence of carbapenemases was suspected in a minority (16.7%) of tested isolates. As safe and eﬀective therapeutic options in carbapenem-resistant Gram-negative infections are severely limited, characterization of these isolates using phenotypic and molecular-based methods is important to provide insights into the epidemiological features of these pathogens.

show abstract

Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test

Cited by 10 publications

References 15 publications

NDM Production as a Dominant Feature in Carbapenem-Resistant Enterobacteriaceae Isolates from a Tertiary Care Hospital

NDM Production as a Dominant Feature in Carbapenem-Resistant Enterobacteriaceae Isolates from a Tertiary Care Hospital

Multidrug Resistant Bacterial Profile and Patterns for Pus Isolates and Recurrent Wound Infections in Nongovernmental Hospitals of Jordan

Insights on carbapenem-resistant Pseudomonas aeruginosa

Contact Info

Product

Resources

About