Detection of Carbapenemase Genes in Aquatic Environments in Rio de Janeiro, Brazil

Araujo, Carlos Felipe Machado de; Silva, Dalton Marcondes; Carneiro, Marcos Tavares; Ribeiro, Sthefanie da Silva; Fontana-Maurell, Marcela; Álvarez, Patricia; Asensi, Marise Dutra; Zahner, Viviane; Carvalho-Assef, Ana Paula D’Alincourt

doi:10.1128/aac.02753-15

Cited by 40 publications

(29 citation statements)

References 18 publications

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“…58 Of concern, bla NDM-1 was found in the water near Rio de Janeiro in the time period leading up to the 2016 Olympics. 59 MBL enzymes have been reported in CPE recovered from patients in various African countries for the past decade or so. However, the current magnitude of the MBLmediated CPE epidemic in Africa is difficult to estimate due to scarcity of data.…”

Section: Where Are Cpe Found?mentioning

confidence: 98%

The global epidemiology of carbapenemase-producing Enterobacteriaceae

2016

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Carbapenemase-producing Enterobacteriaceae (CPE) are an important and increasing threat to global health. Both clonal spread and plasmid-mediated transmission contribute to the ongoing rise in incidence of these bacteria. Among the 4 classes of β-lactamases defined by the Ambler classification system, the carbapenemases that confer carbapenem resistance in Enterobacteriaceae belong to 3 of them: Class A (K. pneumoniae carbapenemases, KPC), Class B (metallo-β-lactamases, MBL including New Delhi metallo-β-lactamases, NDM) and Class D (OXA-48-like carbapenemases). KPC-producing CPE are the most commonly occurring CPE in the United States. MBL-producing CPE have been most commonly associated with the Indian Subcontinent as well as with specific countries in Europe, including Romania, Denmark, Spain, and Hungary. The epicenter of OXA-48-like-producing is in Turkey and surrounding countries. Detailed knowledge of the epidemiology and molecular characteristics of CPE is essential to stem the spread of these pathogens.

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Section: Where Are Cpe Found?mentioning

confidence: 98%

The global epidemiology of carbapenemase-producing Enterobacteriaceae

2016

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“…bla IMP-8 and bla VIM-1 were found on IncFIB type plasmids capable of transconjugation while bla VIM-34 was suspected to be chromosomally integrated [Kieffer 2016]. As reported in 2016, water collected from Rio De Janeiro yielded many bla KPC producing Enterobacteriaceae as well as bla GES , bla OXA-48-like , and bla NDM genes amplified directly from aquatic microbial communities [de Araujo 2016]. Routine environmental sampling could prove advantageous in anticipating spread of novel antibiotic resistance genes into clinical pathogens.…”

Section: Non-infectious Surveillancementioning

confidence: 99%

The rapid spread of carbapenem-resistant Enterobacteriaceae

Potter

D’Souza

Dantas

2016

Drug Resistance Updates

320

211

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Carbapenems, our one-time silver bullet for multidrug resistant bacterial infections, are now threatened by widespread dissemination of carbapenem-resistant Enterobacteriaceae (CRE). Successful expansion of Enterobacteriaceae clonal groups and frequent horizontal gene transfer of carbapenemase expressing plasmids are causing increasing carbapenem resistance. Recent advances in genetic and phenotypic detection facilitate global surveillance of CRE diversity and prevalence. In particular, whole genome sequencing enabled efficient tracking, annotation, and study of genetic elements colocalized with carbapenemase genes on chromosomes and on plasmids. Improved characterization helps detail the co-occurrence of other antibiotic resistance genes in CRE isolates and helps identify pan-drug resistance mechanisms. The novel β-lactamase inhibitor, avibactam, combined with ceftazidime or aztreonam, is a promising CRE treatment compared to current colistin or tigecycline regimens. To halt increasing CRE-associated morbidity and mortality, we must continue quality, cooperative monitoring and urgently investigate novel treatments.

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“…Indeed, KPC-type CPOs have been found in hospital effluents in Brazil, China, and the United States (Chagas et al, 2011;Zhang et al, 2012;Picao et al, 2013;Woodford et al, 2014;Weingarten et al, 2018). Subsequently, identification of the presence of CPO in polluted aquatic environments such as recreational waters indicated a potential threat to beach frequenters (de Araujo et al, 2016;Paschoal et al, 2017;Leonard et al, 2018).…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Potential KPC‐2 carbapenemase reservoir of environmental Aeromonas hydrophila and Aeromonas caviae isolates from the effluent of an urban wastewater treatment plant in Japan

Sekizuka

Inamine

Segawa

et al. 2019

Environ Microbiol Rep

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Summary Aeromonas hydrophila and Aeromonas caviae adapt to saline water environments and are the most predominant Aeromonas species isolated from estuaries. Here, we isolated antimicrobial‐resistant (AMR) Aeromonas strains (A. hydrophila GSH8‐2 and A. caviae GSH8M‐1) carrying the carabapenemase blaKPC‐2 gene from a wastewater treatment plant (WWTP) effluent in Tokyo Bay (Japan) and determined their complete genome sequences. GSH8‐2 and GSH8M‐1 were classified as newly assigned sequence types ST558 and ST13, suggesting no supportive evidence of clonal dissemination. The strains appear to have acquired blaKPC‐2‐positive IncP‐6‐relative plasmids (pGSH8‐2 and pGSH8M‐1‐2) that share a common backbone with plasmids in Aeromonas sp. ASNIH3 isolated from hospital wastewater in the United States, A. hydrophila WCHAH045096 isolated from sewage in China, other clinical isolates (Klebsiella, Enterobacter and Escherichia coli), and wastewater isolates (Citrobacter, Pseudomonas and other Aeromonas spp.). In addition to blaKPC‐2, pGSH8M‐1‐2 carries an IS26‐mediated composite transposon including a macrolide resistance gene, mph(A). Although Aeromonas species are opportunistic pathogens, they could serve as potential environmental reservoir bacteria for carbapenemase and AMR genes. AMR monitoring from WWTP effluents will contribute to the detection of ongoing AMR dissemination in the environment and might provide an early warning of potential dissemination in clinical settings and communities.

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Detection of Carbapenemase Genes in Aquatic Environments in Rio de Janeiro, Brazil

Cited by 40 publications

References 18 publications

The global epidemiology of carbapenemase-producing Enterobacteriaceae

The global epidemiology of carbapenemase-producing Enterobacteriaceae

The rapid spread of carbapenem-resistant Enterobacteriaceae

Potential KPC‐2 carbapenemase reservoir of environmental Aeromonas hydrophila and Aeromonas caviae isolates from the effluent of an urban wastewater treatment plant in Japan

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