Detection of carbapenemase- and ESBL-producing Klebsiella pneumoniae from bovine bulk milk and comparison with clinical human isolates in Italy

Bonardi, S.; Cabassi, Clotilde Silvia; Fiaccadori, Enrico; Cavirani, Sandro; Parisi, Antonio; Bacci, C.; Lamperti, L.; Rega, Martina; Conter, M.; Marra, F.; Crippa, Cecilia; Gambi, L.; Spadini, Costanza; Iannarelli, Mattia; Paladini, C.; Filippin, N.; Pasquali, Frédérique

doi:10.1016/j.ijfoodmicro.2022.110049

Cited by 9 publications

(4 citation statements)

References 47 publications

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“…Since in the present study, EBSL was detected only in K. pneumoniae, it has been reported more frequently in recent years that K. pneumoniae can develop multiple mechanisms to become resistant to antibiotics including the production of antibiotic inactivating enzymes such as β-lactamases and ESBLs [21]. This finding is comparable to what has been found in different studies from other countries in bulk-tank milk samples [12,20,22], and in milk samples from cows with bovine mastitis [23][24][25]. In Colombia, ESBL of the SHV type has already been detected in K. pneumoniae in clinical patients [26][27][28], in community environments [29], also in Salmonella from retail broiler meat samples [30], and in E. coli from different sources in the poultry production [31].…”

Section: Discussionsupporting

confidence: 88%

“…It should be noted that ESBL-producing organisms, which might be present as minor constituents of the total bacterial microbiota in tank milk, could potentially go undetected due to the lower sensitivity (10 CFU/mL) of this approach. This stands in contrast to the selective enrichment method which has been previously employed for tank milk samples in various reports [11,12,20]; large differences in sensitivity are to be expected between these methods. The results of this study suggest that the sensitivity of this non-selective method for detecting ESBLs might be comparatively lower.…”

Section: Microbiological Isolation and Species Identification Of Kleb...mentioning

confidence: 82%

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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae and risk factors associated with high total bacterial count in bulk tank milk from dairy farms in Colombia

Campos,

Akineden,

Fernández-Silva

et al. 2024

Braz J Microbiol

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The objective of the study was to evaluate the frequency and genetic characteristics of ESBL-producing Escherichia coli and Klebsiella spp. and the risk factors associated with a high total bacterial count in bulk tank milk samples of dairy farms in three municipalities of the Antioquia Department, Colombia. Fifteen samples were positive for E. coli and Klebsiella spp. Subsequent analysis of the 16 S rRNA gene sequences confirmed these isolates included E. coli (n = 3), K. oxytoca (n = 11), and K. pneumoniae (n = 1). None of the isolates was positive for ESBL identification by phenotypic methods, but the only the isolate of K. pneumoniae was positive for the blaSHV61 gene by sequence analysis. The antibiotic susceptibility evaluation for all Klebsiella spp. isolates identified resistance to fosfomycin (50%; 6/12) and ampicillin (100%; 12/12). While most of the herds maintain adequate hygienic quality, specific risk factors such as having more than 60 milking cows, frequent changes in milkers, milking in paddocks, and using a chlorinated product for pre-dipping have been identified as associated with a high total bacterial count > 100,000 CFU/mL in bulk tank milk. However, certain variables including the milker being the owner of the animals and the proper washing and disinfection of the milking machine contribute to maintain a high level of hygiene and quality in the raw milk stored in the tanks. In conclusion, the frequency of ESBL producers was relatively low, with only K. pneumoniae testing positive for the blaSHV ESBL type. The presence of these bacteria in milk tanks represents a potential risk to public health for consumers of raw milk and its derivatives.

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Section: Discussionsupporting

confidence: 88%

Section: Microbiological Isolation and Species Identification Of Kleb...mentioning

confidence: 82%

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae and risk factors associated with high total bacterial count in bulk tank milk from dairy farms in Colombia

Campos,

Akineden,

Fernández-Silva

et al. 2024

Braz J Microbiol

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“…KPC-producing isolates represent an important public health issue. Recently, emerging data on food-producing animals and their products, as reservoirs for KPC-producing isolates, are also being reported in different countries [ 22 , 23 ]. New beta-lactams/beta-lactamase inhibitors are now considered a first-line treatment option against KPC-producing isolates.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Costantino,

Principe,

Mehat

et al. 2024

Antibiotics

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Infections caused by KPC-producing K. pneumoniae continue to pose a significant clinical challenge due to their emerging resistance to new antimicrobials. We investigated the association between two drugs whose roles have been repurposed against multidrug-resistant bacteria: fosfomycin and temocillin. Temocillin exhibits unusual stability against KPC enzymes, while fosfomycin acts as a potent “synergizer”. We conducted in vitro antimicrobial activity studies on 100 clinical isolates of KPC-producing K. pneumoniae using a combination of fosfomycin and temocillin. The results demonstrated synergistic activity in 91% of the isolates. Subsequently, we assessed the effect on Galleria mellonella larvae using five genetically different KPC-Kp isolates. The addition of fosfomycin to temocillin increased larvae survival from 73 to 97% (+Δ 32%; isolate 1), from 93 to 100% (+Δ 7%; isolate 2), from 63 to 86% (+Δ 36%; isolate 3), from 63 to 90% (+Δ 42%; isolate 4), and from 93 to 97% (+Δ 4%; isolate 10). Among the temocillin-resistant KPC-producing K. pneumoniae isolates (24 isolates), the addition of fosfomycin reduced temocillin MIC values below the resistance breakpoint in all isolates except one. Temocillin combined with fosfomycin emerges as a promising combination against KPC-producing K. pneumoniae, warranting further clinical evaluation.

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“…CTX-M-15-producing K. pneumoniae ST307 has often been isolated from humans worldwide and is increasingly found in various animal species as well [5,[9][10][11][12][13][14][15][16]. In Europe, it was first isolated from an African spurred tortoise (Centrochelys sulcata) in the UK in 2019, followed by several reports on the isolation in other species, such as cats, dogs, horses, cattle, and birds [17][18][19][20][21][22][23][24]. Because the importation of live exotic companion animals is still at a high level, fundamental research on the potential risk posed by the dissemination of antimicrobial-resistant bacteria seems to be justified [25].…”

Section: Introductionmentioning

confidence: 99%

Genomic and Functional Characterization of CTX-M-15-Producing Klebsiella pneumoniae ST307 Isolated from Imported Leopard Tortoises in Germany

Schmidt,

Aurich,

Unger

et al. 2024

Applied Microbiology

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The Klebsiella pneumoniae ST307 clone, identified in the mid-1990s, has emerged as a global antimicrobial-resistant (AMR) high-risk clone, significantly contributing to the global health challenge also posed by other AMR K. pneumoniae lineages. The acquisition of a blaCTX-M-15-carrying plasmid has facilitated its widespread dissemination. At Europe’s major transport hub for the movement of live animals, Frankfurt Airport, a shipment of 20 live leopard tortoises was sampled during German border control in 2014. Phylogenetic analysis (MLST) identified a K. pneumoniae ST307 strain, prompting further investigation. Our analysis revealed the presence of a ~193 kb plasmid carrying a broad range of AMR genes, including blaCTX-M-15, blaTEM-1B, blaOXA-1, aac(3)-IIa, aac(6′)-Ib-cr, aph(3″)-Ib, aph(6)-Id, and qnrB1. Additionally, mutations in the quinolone resistance-determining region in gyrA (S83I) and parC (S80I) were detected. Phenotypic testing demonstrated resistance of the isolate to the most common antimicrobials used in both human and veterinary medicine; exceptions included carbapenems and newer β-lactamase inhibitor combinations. Because the role of imported exotic animals in the dissemination of AMR genes is largely deficient, the present study fills yet missing mosaic pieces in the complete picture of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales.

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Detection of carbapenemase- and ESBL-producing Klebsiella pneumoniae from bovine bulk milk and comparison with clinical human isolates in Italy

Cited by 9 publications

References 47 publications

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae and risk factors associated with high total bacterial count in bulk tank milk from dairy farms in Colombia

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae and risk factors associated with high total bacterial count in bulk tank milk from dairy farms in Colombia

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Genomic and Functional Characterization of CTX-M-15-Producing Klebsiella pneumoniae ST307 Isolated from Imported Leopard Tortoises in Germany

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