Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples

Hasan, Hussam Mahmood; Jasim, Hameed M.; Salih, Ghada Mohammed

doi:10.21608/ejhm.2022.269974

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Effect of FLAB filtrate on the expression of fimH, mrkD, NDM-1, and OXA-1 was investigated according to Carey et al (14) . The most virulent isolate of K. pneumoniae obtained from previous study (15) was selected for this purpose as it was resistant to an extensive range of antibiotics, and the efficient biofilm producer .This isolate was incubated with FLAB filtrate at different incubation periods (3, 6, and 9 hrs.…”

Section: Expression Of K Pneumoniae Virulence Genesmentioning

confidence: 99%

Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Hasan¹,

Salih²,

Jasim³

2022

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

Background: Fructophilic lactic acid bacteria (FLAB) are a group of LAB with unique growth features and are regarded as potential bioactive compound manufacturers. FLAB is discovered in D-fructose rich niches that favors D-fructose as a growth substrate triumphs over D-glucose. On D-glucose, they require electron acceptors to proliferate. The organisms have similar metabolic processes for carbohydrates. Fructobacillus spp. are Lactobacillus kunkeei, are members of this unique group. Methodology: Inhibitory effect of filtrate from four FLAB isolates from honeybee was detected against the growth of pathogenic Klebsiella pneumoniae isolates. Biofilm formation, and the expression of fimH, mrkD, and NDM-1, and OXA-1 virulence genes were also tested. Results: It was found that FLAB has antibacterial and antibiofilm effect. The incubation of K. pneumoniae with FLAB suspension at different three-time intervals (3, 6, and 9 hrs.) decreased expression of fimH, mrkD, NDM-1, and OXA-1 virulence genes compared with the expression of the same genes in the absence of FLAB. Conclusion: all FLAB filtrate isolates tested, K. pneumoniae fimH, mrkD, NDM-1, and OXA-1 virulence gene expression was down-regulated, when compared to the control culture.

show abstract

Section: Expression Of K Pneumoniae Virulence Genesmentioning

confidence: 99%

Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Hasan¹,

Salih²,

Jasim³

2022

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

show abstract

Process optimization of malachite green degradation by mixed biofilm positive bacteria: Application of Box–Behnken designs, RSM and whole genome sequences analysis

Aljarad,

Kouidhi,

Ayed

et al. 2023

Biochemical Engineering Journal

View full text Add to dashboard Cite

Detection of fim H and UDP virulence-associated genes in patients with MDR Klebsiella pneumoniae isolates from Baghdad hospitals

Shamsee,

Ibrahim

2024

APJMBB

View full text Add to dashboard Cite

Klebsiella pneumoniae is a gram-negative, encapsulated, and lactose-fermenting bacterium related to hospital-acquired infections that can cause various diseases. The current study aimed to detect the presence of the virulence-associated genes fim H and UDP among multiple drug-resistant K. pneumoniae isolates obtained from different sources using polymerase chain reaction (PCR) to determine their association with antibiotic resistance. Ninety K. pneumoniae isolates were obtained from 210 different clinical samples collected from various sources (urine, sputum, wound swabs, blood, and burns) from patients of both genders in Baghdad hospitals between March 2022 and July 2022. Bacterial identification was based on selective and differential media, followed by seven biochemical tests, the Vitek-2 system, and molecular identification tests were performed using 16S rRNA. The antibiotic susceptibility of isolates to 13 antibiotics was assessed by the disk diffusion method on Mueller–Hinton agar. The results showed that 100% of the isolates were resistant to vancomycin. Otherwise, colistin had the lowest resistance rate (25.55%). The virulence genes fim H and UDP (uge) were detected by multiplex polymerase chain reaction (PCR) in multidrug-resistant (MDR) isolates. The most prevalent gene was fim H (88%) followed by UDP (80%). The virulence genes were highly prevalent among MDR K. pneumoniae isolates. The resistance rate to colistin was 25.55%, making it the most effective antibiotic used in the current study. Vancomycin, on the other hand, had a high resistance value. Multidrug resistant isolates were found to be associated with the presence of virulence genes.

show abstract

Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples

Cited by 3 publications

References 14 publications

Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Process optimization of malachite green degradation by mixed biofilm positive bacteria: Application of Box–Behnken designs, RSM and whole genome sequences analysis

Detection of fim H and UDP virulence-associated genes in patients with MDR Klebsiella pneumoniae isolates from Baghdad hospitals

Contact Info

Product

Resources

About