Detection of canine and equine procalcitonin for sepsis diagnosis in veterinary clinic by the development of novel MIP-based SPR biosensors

Battaglia, Federica; Baldoneschi, Veronica; Meucci, Valentina; Intorre, Luigi; Minunni, Maria; Scarano, Simona

doi:10.1016/j.talanta.2021.122347

Cited by 30 publications

(17 citation statements)

References 39 publications

(57 reference statements)

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“…3), and the imprinting factor (α = Q MIP /Q NIP ) was calculated to be 3.2. In recent years, the self-polymerization of dopamine under mild reaction conditions (weak alkaline pH at room temperature) was applied to prepare MIPs for various target molecules [19][20][21][22][23]. The technique was also valid for preparing MIP membrane to recognize quercetin in this study.…”

Section: Adsorption Ability Of Mip-ht and Nip-ht For Quercetinmentioning

confidence: 97%

“…Recently, it was reported that dopamine successfully forms polydopamine films onto a variety of object by self-polymerization in an aqueous solution at weak alkaline pH at room temperature [18]. The technique of dopamine self-polymerization under mild conditions has been applied to prepare various polydopamine-based MIPs [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Development of enzyme/titanate nanosheet complex coated with molecularly imprinted polydopamine for colorimetric quercetin assay

et al. 2022

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A novel hybrid material, which is an enzyme/inorganic nanosheet complex coated by molecularly imprinted polymer (MIP), was developed, and applied to colorimetric quercetin assay. First, an enzyme/inorganic nanosheet complex was prepared from horseradish peroxidase (HRP) enzyme and titanate nanosheet (TiOx), using electrostatic interactions between them in acetate buffer. In the next place, dopamine self-polymerization was performed in the presence of HRP/TiOx complex with quercetin as a template, to prepare MIP membrane onto the HRP/TiOx complex. After washing process, a new hybrid material, MIP-coated HRP/TiOx complex (MIP-HT) was obtained. MIP-HT adsorbed quercetin efficiently, compared with NIP-HT that is an HRP/TiOx complex coated with non-imprinted polydopamine. MIP-HT showed enzymatic activity for an oxidation reaction of guaiacol, which is a chromogenic substrate of HRP, whereas the enzymatic activity of NIP-HT was significantly suppressed. The amount of brown product, formed by the color reaction, reduced owing to the presence of quercetin in sample solution, and a good liner relationship was observed between the concentration of quercetin and the increment of absorbance at 470 nm. The investigation using several biomolecules indicates that MIP-HT has the ability to detect quercetin and its analogues with selectivity. Therefore, MIP-HT shows great promise as a new and attractive material for use in colorimetric assay of quercetin or quercetin analogues.

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Section: Adsorption Ability Of Mip-ht and Nip-ht For Quercetinmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Development of enzyme/titanate nanosheet complex coated with molecularly imprinted polydopamine for colorimetric quercetin assay

et al. 2022

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“…Due to the aforementioned and further extremely advantageous features, our research group started working with PDA and developed several smart optical-based assays [35][36][37][38] and MIP-based mimetic receptors for biosensing [39]. More recently, also polynorepinephrine (PNE), a close analog of PDA, has displayed improved analytical performances when exploited for MIP synthesis, giving even better results than PDA [40][41][42]. Catecholamine-based MIPs, in particular PDA and PNE, are very attractive since their polymerization (the mechanistic processes are currently not completely known [43][44][45][46][47]) is obtained spontaneously and under mild aqueous conditions starting from the corresponding low-cost monomers (dopamine (DA) and norepinephrine (NE), respectively), and can be performed in any laboratory as they do not require specific technology (being easy to operate) or expensive devices.…”

Section: Introductionmentioning

confidence: 99%

A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates

et al. 2022

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An original biomimetic enzyme-linked immunoassay (BELISA) to target the small peptide hormone gonadorelin is presented. This peptide has been recently listed among the substances banned in sports by the World Antidoping Agency (WADA) since its misuse by male athletes triggers testosterone increase. Hence, in response to this emerging issue in anti-doping controls, we proposed BELISA which involves the growth of a polynorepinephrine (PNE)–based molecularly imprinted polymer (MIP) directly on microwells. PNE, a polydopamine (PDA) analog, has recently displayed impressive performances when it was exploited for MIP preparation, giving even better results than PDA. Gonadorelin quantification was accomplished via a colorimetric indirect competitive bioassay involving the competition between biotinylated gonadorelin linked to the signal reporter and the unlabeled analyte. These compete for the same MIP binding sites resulting in an inverse correlation between gonadorelin concentration and the output color signal (λ = 450 nm). A detection limit of 277 pmol L−1 was achieved with very good reproducibility in standard solutions (avCV% = 4.07%) and in urine samples (avCV% = 5.24%). The selectivity of the assay resulted adequate for biological specimens and non-specific control peptides. In addition, the analytical figures of merit were successfully validated by mass spectrometry, the reference anti-doping benchtop platform for the analyte. BELISA was aimed to open real perspectives for PNE-based MIPs as alternatives to antibodies, especially when the target analyte is a poorly or non-immunogenic small molecule, such as gonadorelin. Graphical abstract

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“…However, recently, a new class of endogenous functional monomers, belonging to the class of catecholamines (CAs), has been successfully tested to produce MIPs against a variety of targets, including proteins, and coupled to different bioanalytical platforms. [20][21][22][23][24] Thanks to its capacity to easily self-polymerize under mild alkaline conditions forming adherent films on virtually all the surfaces, [25] polydopamine (PDA) is undoubtedly the leading example within polycatecholamines (pCAs). Indeed, following pioneering investigations and applications, [26][27][28] it has generated an impressive amount of data ranging from material sciences, to bioanalytics and biosensing.…”

Section: Introductionmentioning

confidence: 99%

Structurally Constrained MUC1‐Tn Mimetic Antigen as Template for Molecularly Imprinted Polymers (MIPs): A Promising Tool for Cancer Diagnostics

et al. 2022

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Abnormal glycoconjugates have distinctly been recognized as potential biomarkers for cancer diagnosis. A great deal of attention has been focused on Tn antigen, an oversimplified mucin-1 O-glycan, over-expressed in different cancers. Herein, we investigate the possibility to replace the use of anti-Tn monoclonal antibodies with an innovative class of catecholamine-based Molecularly Imprinted Polymers (MIPs), emerging in recent years as promising tools for bioanalytical applications. MIPs are synthetic receptors characterized by high sensitivity and specificity towards the imprinted target. Here, original polynorepinephrine-based MIPs coupled to Surface Plasmon Resonance biosensing for Tn antigen recognition are reported.We have verified the imprinting and binding capacity of these MIPs towards very small antigenic entities, represented by the natural Tn antigen and the TnThr mimetic 1 (conjugated to BSA or linked to a MUC1 hexapeptide analogue), and compared the biosensor performances with an anti-Tn monoclonal antibody. The results clearly display the effectiveness of the pursued imprinting strategies.

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Detection of canine and equine procalcitonin for sepsis diagnosis in veterinary clinic by the development of novel MIP-based SPR biosensors

Cited by 30 publications

References 39 publications

Development of enzyme/titanate nanosheet complex coated with molecularly imprinted polydopamine for colorimetric quercetin assay

Development of enzyme/titanate nanosheet complex coated with molecularly imprinted polydopamine for colorimetric quercetin assay

A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates

Structurally Constrained MUC1‐Tn Mimetic Antigen as Template for Molecularly Imprinted Polymers (MIPs): A Promising Tool for Cancer Diagnostics

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