Detection of brucella antigen in bovine fetal stomach contents by agar gel precipitation and counter-immunoelectrophoresis

Chand, Puran; Gupta, Rajat; Khanna, R. N. S.; Sadana, J. R.

doi:10.1016/s0034-5288(18)30760-4

Cited by 4 publications

(6 citation statements)

References 6 publications

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“…Recently, detection of brucella antigen in fetal stomach contents of sheep (Mahajan & Kulshreshtha 1986) and cattle (Chand et al 1987) have been reported. Accordingly, the CIE has been found to be a more specific, sensitive and rapid procedure than the culture isolation in the diagnosis of bacterial meningitis (Rytel 1979) and cattle brucellosis (Chand et al 1987).…”

Section: Discussionmentioning

confidence: 99%

“…All the 14 cases from which fetuses were obtained belonged to an organized buffalo farm. The animals at this farm are kept in sheds which are in close proximity with cattle sheds and Brucella abortus biotype 3 has been isolated from these cattle (Chand et al 1987).…”

Section: L I N I C a L Specimensmentioning

confidence: 99%

“…Hyperimmune serum against Br. abortus S-99 was prepared in rabbits by the method of Corbel et al (1983) while that against sonicated extract of S-99 was raised as described earlier (Chand et al 1987). Both sera were stored separately at -20°C using 1 : 10 000 merthiolate as preservative.…”

Section: Hyperimmune Serummentioning

confidence: 99%

“…The stomach contents from all the aborted fetuses were processed as described earlier (Chand et al 1987). Briefly, approximately 10 ml of 0.15 mol/l sodium chloride solution was added to an equal volume of stomach contents and mixed thoroughly with the help of a magnetic stirrer for 15-20 min.…”

Section: Agar Gel Precipitation Testmentioning

confidence: 99%

“…Much progress has been reported during the past few years in reducing bovine brucellosis in many countries through standardization of traditional methods and application of new procedures (Farina 1985). Among the new procedures, counter-immunoelectrophoresis (CIE) has been employed for the detection of brucella antibodies in sheep (Muhammed et al 1980) and antigen in fetal stomach contents of sheep (Mahajan & Kulshreshtha 1986) and cattle (Chand et al 1987).…”

mentioning

confidence: 99%

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Counter‐immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes

Chand¹,

Khanna²,

Sadana³

1988

Journal of Applied Bacteriology

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Counter-immunoelectrophoresis was employed for the detection of Brucella antigen in stomach contents of aborted buffalo fetuses and antibody in aborted as well as apparently healthy in contact buffaloes. Five of 16 aborted cases were serologically positive for brucellosis but isolation of Brucella abortus was successful in only two cases. By counter-immunoelectrophoresis, Brucella antigen was detected in the fetal stomach contents of four serologically positive cases. Of the 68 serum samples from in contact healthy buffaloes, 10 were positive with counter-immunoelectrophoresis: more than were detected by tube agglutination, Rose Bengal plate agglutination, complement fixation and agar gel precipitation test.

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Section: Discussionmentioning

confidence: 99%

Section: L I N I C a L Specimensmentioning

confidence: 99%

Section: Hyperimmune Serummentioning

confidence: 99%

Section: Agar Gel Precipitation Testmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Counter‐immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes

Chand¹,

Khanna²,

Sadana³

1988

Journal of Applied Bacteriology

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Use of hydrophobic cloths as antibody adsorbents for enzyme immunoassay: Detection of Brucella antigens

Blais

Yamazaki

Rigby

1989

Veterinary Microbiology

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Comparison of Dot Enzyme‐linked Immunosorbent Assay (Dot‐ELISA) with other Serological Tests for the Detection of Brucella Antibodies in Bovine Sera

Chand

Sadana

Batra

et al. 1989

Journal of Veterinary Medicine, Series B

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A dot Enzyme-linked Immunosorbent Assay (dot-ELISA), using whole cell Erucelka abortus antigen dotted on the nitrocellulose membrane bound to a plastic strip (dipstick) was employed for the detection of Erucella antibodies in bovine sera. The results were compared with that of serum agglutination (SAT), Rose Bengal plate agglutination (RBPT) and Complement Fixation test (CFT). All the four tests gave negative reaction in 127 sera obtained from a brucellosis free herd. Testing of 549 sera from a chronically infected herd revealed 57 positive and 447 negative animals in all the four assays. Of the remaining 45 sera, 34 were positive in dot-ELISA. Six of these cases were independently detected by dot-ELISA while 28 showed positive reactions in combination with other tests. When serum samples from 158 aborted cases were subjected to dot-ELISA, 79 were found positive. Of these dot-ELISA positive cases, 71 gave positive reaction in SAT, 72 in RBPT and 78 in CFT. E. abortus biotype 3 was isolated from 34 of the 98 aborted fetuses examined.

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Detection of brucella antigen in bovine fetal stomach contents by agar gel precipitation and counter-immunoelectrophoresis

Cited by 4 publications

References 6 publications

Counter‐immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes

Counter‐immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes

Use of hydrophobic cloths as antibody adsorbents for enzyme immunoassay: Detection of Brucella antigens

Comparison of Dot Enzyme‐linked Immunosorbent Assay (Dot‐ELISA) with other Serological Tests for the Detection of Brucella Antibodies in Bovine Sera

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