Detection of breast cancer-related point-mutations using screen-printed and gold-plated electrochemical sensor arrays suitable for point-of-care applications

Thoeny, Vanessa; Melnik, Eva; Asadi, Malahat; Mehrabi, Pooyan; Schalkhammer, Thomas; Pulverer, Walter; Maier, Thomas; Mutinati, Giorgio C.; Lieberzeit, Peter A.; Hainberger, Rainer

doi:10.1016/j.talo.2022.100150

Cited by 9 publications

(4 citation statements)

References 48 publications

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“…Multiplexing and arrayed platforms allow concurrent analysis of different analytes using several individually addressable electrodes on the same chip. Companies are developing high-density sensor arrays with thousands of electrodes for massively parallel measurements[ 45 ]. Multiplexed data provides better predictive power but also necessitates advanced analytics.…”

Section: The Path Forwardmentioning

confidence: 99%

Leveraging electrochemical sensors to improve efficiency of cancer detection

Fu,

Karimi-Maleh

2024

World J Clin Oncol

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Electrochemical biosensors have emerged as a promising technology for cancer detection due to their high sensitivity, rapid response, low cost, and capability for non-invasive detection. Recent advances in nanomaterials like nanoparticles, graphene, and nanowires have enhanced sensor performance to allow for cancer biomarker detection, like circulating tumor cells, nucleic acids, proteins and metabolites, at ultra-low concentrations. However, several challenges need to be addressed before electrochemical biosensors can be clinically implemented. These include improving sensor selectivity in complex biological media, device miniaturization for implantable applications, integration with data analytics, handling biomarker variability, and navigating regulatory approval. This editorial critically examines the prospects of electrochemical biosensors for efficient, low-cost and minimally invasive cancer screening. We discuss recent developments in nanotechnology, microfabrication, electronics integration, multiplexing, and machine learning that can help realize the potential of these sensors. However, significant interdisciplinary efforts among researchers, clinicians, regulators and the healthcare industry are still needed to tackle limitations in selectivity, size constraints, data interpretation, biomarker validation, toxicity and commercial translation. With committed resources and pragmatic strategies, electrochemical biosensors could enable routine early cancer detection and dramatically reduce the global cancer burden.

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Section: The Path Forwardmentioning

confidence: 99%

Leveraging electrochemical sensors to improve efficiency of cancer detection

Fu,

Karimi-Maleh

2024

World J Clin Oncol

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“…[ 98 ] Porous noble metal‐based nanoenzymes can efficiently drive this reaction, enabling colorimetric detection associated with H 2 O 2 . [ 99 ] For example, Viswanathan et al synthesized nanoporous silver (np‐Ag) microcubes and coated them with polyelectrolytes (PE) to detect glucose. [ 100 ] By catalyzing the oxidation of glucose and TMB, the concentration of the target glucose can be quantitatively determined via the color change.…”

Section: Porous Noble Metal‐based Nanomaterials For Electrochemical A...mentioning

confidence: 99%

Porous Noble Metal‐Based Nanomaterials in Biomedical Applications

Zhang,

Yang,

et al. 2023

Advanced NanoBiomed Research

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Noble metal‐based nanomaterials have attracted tremendous attention in biomedical applications due to their unique electrical, optical, and chemical properties, playing crucial roles in the ultrasensitive detection of biomarkers, bioimaging, cancer therapy, etc. Especially, porous noble metal‐based nanomaterials show superior performance due to the large specific area and multiple active sites. Platforms constructed from porous noble metal‐based nanomaterials are emerging as highly promising tools for various biomedical applications. Herein, the properties and synthesis strategies of porous noble metal‐based nanomaterials are briefly introduced. Then the recent progress of porous noble metal‐based nanomaterials in the biomedical field is highlighted, focusing primarily on their applications in optics, electrochemistry, and mass spectrometry. Finally, the challenges related to fabrication and biocompatibility for their applications while also providing an outlook on their widespread use in clinical situations are discussed. This review aims to provide further insights into the design of porous noble metal‐based nanomaterials and expand their applications in the biomedical field.

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“…Electrochemical sensors are reliable transducers, can be produced cost-effectively using roll-to-roll screen printing, and are suitable for a wide range of analytes in various medical and environmental applications [ 2 , 3 , 4 , 5 ]. The assays used for detection usually differ only marginally from the assays used in gold standard methods such as microarrays, lateral flow tests, or enzyme-linked immunosorbent assays [ 6 , 7 , 8 , 9 , 10 , 11 ]. This is because of the high selectivity and specificity of the established and well-researched assays, which, however, may require sample preparation steps (e.g., target analyte extraction, sample dilution) and target analyte preparation steps (e.g., the addition of chemicals, proteins, enzymes, or antibodies).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Diffusion Study in Poly(Ethylene Glycol) Dimethacrylate-Based Hydrogels

Melnik,

Kurzhals,

Mutinati

et al. 2024

Sensors

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Hydrogels are of great importance for functionalizing sensors and microfluidics, and poly(ethylene glycol) dimethacrylate (PEG-DMA) is often used as a viscosifier for printable hydrogel precursor inks. In this study, 1–10 kDa PEG-DMA based hydrogels were characterized by gravimetric and electrochemical methods to investigate the diffusivity of small molecules and proteins. Swelling ratios (SRs) of 14.43–9.24, as well as mesh sizes ξ of 3.58–6.91 nm were calculated, and it was found that the SR correlates with the molar concentration of PEG-DMA in the ink (MCI) (SR = 0.1127 × MCI + 8.3256, R2 = 0.9692) and ξ correlates with the molecular weight (Mw) (ξ = 0.3382 × Mw + 3.638, R2 = 0.9451). To investigate the sensing properties, methylene blue (MB) and MB-conjugated proteins were measured on electrochemical sensors with and without hydrogel coating. It was found that on sensors with 10 kDa PEG-DMA hydrogel modification, the DPV peak currents were reduced to 92 % for MB, 73 % for MB-BSA, and 23 % for MB-IgG. To investigate the diffusion properties of MB(-conjugates) in hydrogels with 1–10 kDa PEG-DMA, diffusivity was calculated from the current equation. It was found that diffusivity increases with increasing ξ. Finally, the release of MB-BSA was detected after drying the MB-BSA-containing hydrogel, which is a promising result for the development of hydrogel-based reagent reservoirs for biosensing.

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Detection of breast cancer-related point-mutations using screen-printed and gold-plated electrochemical sensor arrays suitable for point-of-care applications

Cited by 9 publications

References 48 publications

Leveraging electrochemical sensors to improve efficiency of cancer detection

Leveraging electrochemical sensors to improve efficiency of cancer detection

Porous Noble Metal‐Based Nanomaterials in Biomedical Applications

Electrochemical Diffusion Study in Poly(Ethylene Glycol) Dimethacrylate-Based Hydrogels

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