Detection of Bovine Papilloma Viruses in Wart-Like Lesions of Upper Gastrointestinal Tract of Cattle and Buffaloes

Abstract

In present investigation, etiopathological characterization of upper gastrointestinal tract (GIT) tumours of cattle and buffaloes was undertaken. A total of 27 GIT wart-like lesions in rumen, reticulum, mouth and oesophagus of cattle and buffaloes revealed the presence of small nodular to larger spherical or slender growths with thin base present on mucosa and ruminal pillar. Histopathologically, these cases were diagnosed as fibropapilloma/papilloma. This is the first world record on ruminal papillomatosis in buffaloes. Ruminal warts of cattle and buffaloes revealed the presence of BPV-5, -1 & -2, which is the first report of presence of these BPVs in the ruminal warts from India. Quantitative real-time PCR revealed that DNA samples of different GIT wart-like lesions contained varying amount of BPV DNA copy numbers. Immunohistochemistry revealed that the PCNA and Ki67 immunopositivity was present in the basal and spinosum layer of the fibropapilloma/papilloma, indicating these as the cellular proliferation site. In conclusion, the present investigation revealed that BPV-5, -1 & -2 are associated with certain ruminal wart-like lesions/growths in cattle and buffaloes, and the basal and spinosum layer of the ruminal fibropapilloma/papilloma were cellular proliferation sites.

Detection and genomic characterization of Bovine papillomavirus isolated from Chinese native cattle

“…Morphological lesions of neoplastic tissues from all the cases were almost identical, and the neoplastic masses consisted of a mixed proliferation of epithelial and mesenchymal components. The neoplastic tissue was composed of sheet‐like growths of squamous epithelial cells, and their surface was moderately hyperkeratotic (Figure ).These histopathological features were similar to what was described for BPV infection (Kumar, Nagarajan, Saikumar, Arya, & Somvanshi, ).…”

Papillomaviruses in ruminants: An update

“…Some studies have shown co‐infection of BPV13 and BPV2 in cattle urothelial bladder tumour cells, as well as BPV13 itself (Roperto, Munday, et al., ). Additionally, BPV1 and BPV2 were detected by qPCR in a wart collected from the reticulum of a buffalo from India (Kumar et al., ). In the case of urinary bladder and gastrointestinal tract cancer, prolonged ingestion of immunosuppressant compounds of bracken fern could explain the ease of multiple BPV types infecting their hosts (Roperto, Munday, et al., ; Roperto, Russo, et al., ).…”

“…Within the large group of ungulates (diverse group of mammals that includes odd‐toed and even‐toed ungulates), bovine papillomavirus (BPV) plays a role in a variety of diseases in domestic and wild ruminants (Bam, Kumar, Leishangthem, Saikia, & Somvanshi, ; Borzacchiello et al., ; Kumar, Nagarajan, Saikumar, Arya, & Somvanshi, ; Lunardi, Alfieri, et al., ; Tomita, Literak, Ogawa, Jin, & Shirasawa, ; Van Dyk et al., ), causing considerable morbidity to the affected animals. The suborder Ruminantia comprises a large and relevant group of terrestrial herbivorous mammals which embrace the Tragulidae, Giraffidae, Antilo‐capridae, Moschidae, Cervidae and Bovidae families that contain at least 200 extant species (Fernández & Vrba, ).…”

Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle

“…Warts have been observed mainly on the skin (Kumar et al 2013a), teats (Maeda et al 2007) and mucosal surfaces (Kumar et al 2013b) of cattle. These BPV-induced lesions are usually benign in nature and regress spontaneously but can progress to cancer under the impact of environmental co-factors.…”