Detection of Bovine Herpesvirus-1 Infection in Bovine Clinical Samples by Direct Fluorescent Antibody Test

Patel, D. R.; Kalyani, I. H.; Trangadia, B. J.; Sharma, Kishan Kumar; Makwana, Pushpa; Desai, Dhruv; Muglikar, Dushyant; Sakhare, Pramod S.

doi:10.20546/ijcmas.2018.711.249

Cited by 6 publications

(8 citation statements)

References 3 publications

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“…Therefore, some laboratory tests are inadequately reliable to exclude the presence of BoHV-1. Although using FAT could be a profitable test for detection of BoHV-1 antigen within a brief period contrasting virus isolation which consumed much time [47], realtime PCR is less in time-consuming with sufficient reliability and have been credited by OIE [28].…”

Section: Discussionmentioning

confidence: 99%

Concurrent detection of bovine viral diarrhoea virus and bovine herpesvirus-1 in bulls’ semen and their effect on semen quality

El-Mohamady

Behour

Rawash

2020

International Journal of Veterinary Science and Medicine

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Reproductive diseases may have destructive effects on the fertility of cattle. Bovine viral diarrhoea virus (BVDV) and bovine herpes virus-1 (BoHV-1) are potent viral pathogens linked to reproduction. Thus, the aim of this study was to utilize raw semen samples for conventional and molecular detection of BVDV and BoHV-1, simultaneously. Additionally, the effect of virus infection on the semen quality of naturally infected bulls has been investigated. Therefore, 40 bulls were employed for semen collection, evaluation and testing for both viruses by virus isolation, direct fluorescent antibody technique (FAT) and SYBR Green real-time PCR assay. In virus isolation results, no cytopathic effect (CPE) was observed for BVDV on cell culture whereas, eight (20%) samples displayed characteristic grape-like clusters of cells for BoHV-1. By direct FAT, 12 (30%) positive BVDV and 8 (20%) positive BoHV-1 samples were confirmed. SYBR Green real-time PCR analysis using 48 h inoculated semen samples revealed 14 (35%) and 8 (20%) positive samples for BVDV and BoHV-1, respectively. Statistical analysis of semen evaluation parameters showed a significant difference between viral-infected and free groups represented by increased sperm abnormalities and decreased sperm motility, liveability and concentration. However, there was no significant difference among BVDV, BoHV-1 and mixedinfected groups. The study concluded that BVDV and/or BoHV-1 infected bulls expressed low semen quality. Real-time PCR was confirmed to be the ideal laboratory assay for detection of both viruses in semen.

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Section: Discussionmentioning

confidence: 99%

Concurrent detection of bovine viral diarrhoea virus and bovine herpesvirus-1 in bulls’ semen and their effect on semen quality

El-Mohamady

Behour

Rawash

2020

International Journal of Veterinary Science and Medicine

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“…The ability to identify the virus in its subclinical condition has altered over the past decade, allowing for a much clearer grasp of the significance of two subpopulations of dogs in the animal populations with which we work: carrier-shedder adult dogs and CHV-latently infected dogs. The improved sensitivity of antibody-based serology assays like direct fluorescent antibody test (Patel et al, 2018) [34] , ELISA based detection kits and nucleic acid-based polymerase chain reaction (PCR) assays (Vala et al, 2020) [55] has increased our clinical investigation of bovine, equine, canine herpesvirus and other canine pathogenic pathogens (Ronsse et al, 2002) [39] . In addition to identifying CHV adult carriers in the general community, this new momentum has enabled veterinarians to screen canines undergoing invasive procedures.…”

Section: Canine Herpesvirus Infectionmentioning

confidence: 99%

Review on important diseases of Dogs: At glance

Solanki¹,

Desai²,

Dalvi³

et al. 2023

Int. J. Vet. Sci. Anim. Husbandry

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Dogs are one of the most accepted pet animals in every household. Increasing urbanization and modernization of human life has created a space where people are living in nuclear family or alone. This has created peoples' perception and acceptance of having pet for companionship. Humandog interaction and close livelihood confinement emerge as crucial threat of outbreaks of zoonotic disease even a dog's diseases. To prevent and control diseases of dogs, it requires proper knowledge of vaccination, education of pet health management and pets' diseases. Therefore, the current review is on describing dog diseases like, Rabies, Canine Parvoviral gastroenteritis, Canine Distemper, Canine Coronaviral infection, Canine Rotavirus infection, Canine Herpesvirus infection, Canine Leptospirosis, Canine Brucellosis, Transmissible venereal tumors (TVT), Kennel cough, Pyoderma at glance which provides concise information regarding the causative agents and other relevant details.

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“…This is despite the fact that other species, such as cattle, buffalo, camels, and pigs, have also been documented to be afflicted by these bacteria, which ultimately results in losses to farmers. Patel et al (2018) [19] conducted a study on the identification of Bovine Herpesvirus-1 infection in bovine clinical samples using the Direct Fluorescent Antibody Test. This test was used to determine whether or not the samples contained the virus.…”

Section: Introductionmentioning

confidence: 99%

An update on prevention and control of livestock diseases in India

Kumbhani¹,

Parasana²,

Thumar³

et al. 2023

Int. J. Vet. Sci. Anim. Husbandry

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Millions of farmers keep livestock for the purpose of milk production, and every year a wide variety of diseases, some of which can be fatal, cause illness in these animals. Even cattle and buffalo that have a significant threat of disease transmission. They are among the most susceptible people to the introduction of zoonotic diseases into the human population. Milk is in danger of running out in the not too distant future since both consumer demand and the dairy business are both on the rise. Dairy products are progressively used in more and more foods. Farmers take a financial hit each year as a result of illnesses that affect milking dairy animals; the scale of these losses can approach millions of dollars at the national level. At both the state and the national level, there needs to be a diseases control policy in order to put a stop to the losses. In addition to this, the tight interaction that exists between humans and the animals that they have domesticated presents the highest risk of the spread of a range of infectious diseases. The state animal department and the government of India need to conduct an active review of the situation across the state because the number of households that have milk animals has increased. They should also give the process of establishing new rules and putting them into practice the utmost significance, both with the purpose of strengthening the regulation of diseases that are prevalent among the population and with the purpose of reducing the risk of zoonotic transmission. The purpose of this review is to identify and put into effect those measures that will contribute significantly to the eradication of the cattle illnesses that are most prevalent. In this review, we provide a summary of the most prevalent livestock diseases, probable zoonotic diseases, and developing diseases, as well as present and potential regulations that ought to be put into place.

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Detection of Bovine Herpesvirus-1 Infection in Bovine Clinical Samples by Direct Fluorescent Antibody Test

Cited by 6 publications

References 3 publications

Concurrent detection of bovine viral diarrhoea virus and bovine herpesvirus-1 in bulls’ semen and their effect on semen quality

Concurrent detection of bovine viral diarrhoea virus and bovine herpesvirus-1 in bulls’ semen and their effect on semen quality

Review on important diseases of Dogs: At glance

An update on prevention and control of livestock diseases in India

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