Detection of bluetongue virus by using bovine endothelial cells and embryonated chicken eggs

Wechsler, S.J.; Luedke, A J

doi:10.1128/jcm.29.1.212-214.1991

Cited by 13 publications

(8 citation statements)

References 4 publications

(4 reference statements)

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“…Intravenous route is widely used and 100–1000 times more sensitive than yolk sac route (Pearson et al. 1985 ; Wechsler and Luedke 1991 ; Clavijo et al. 2000 ; Dadhich 2004 ).…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

“…Baby hamster kidney-21 (BHK-21), African green monkey (Vero) and mouse L cell lines are commonly used for growth and maintenance of BTV. Wechsler and Luedke ( 1991 ) had successfully propagated the BTV in calf pulmonary artery endothelial (CPAE) cell lines. They also reported that CPAE was more sensitive than 13 other cell lines.…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

“…BTV can be confirmed from culture medium by sandwich ELISA and VNT (Wechsler et al. 1990 ; Wechsler and Luedke 1991 ; Sperlova and Zendulkova 2011 ).…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

“…1970 ; St George et al. 1978 ; Sundin and Mecham 1989 ; Wechsler and Luedke 1991 ). Sometimes, BTV can also be isolated by inoculating laboratory animals, such as mice or hamsters.…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

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An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India

Saminathan

Singh

Khorajiya

et al. 2020

Veterinary Quarterly

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Bluetongue (BT) is an economically important, non-contagious viral disease of domestic and wild ruminants. BT is caused by BT virus (BTV) and it belongs to the genus Orbivirus and family Reoviridae. BTV is transmitted by Culicoides midges and causes clinical disease in sheep, white-tailed deer, pronghorn antelope, bighorn sheep, and subclinical manifestation in cattle, goats and camelids. BT is a World Organization for Animal Health (OIE) listed multispecies disease and causes great socio-economic losses. To date, 28 serotypes of BTV have been reported worldwide and 23 serotypes have been reported from India. Transplacental transmission (TPT) and fetal abnormalities in ruminants had been reported with cell culture adopted live-attenuated vaccine strains of BTV. However, emergence of BTV-8 in Europe during 2006, confirmed TPT of wild-type/field strains of BTV. Diagnosis of BT is more important for control of disease and to ensure BTV-free trade of animals and their products. Reverse transcription polymerase chain reaction, agar gel immunodiffusion assay and competitive enzyme-linked immunosorbent assay are found to be sensitive and OIE recommended tests for diagnosis of BTV for international trade. Control measures include mass vaccination (most effective method), serological and entomological surveillance, forming restriction zones and sentinel programs. Major hindrances with control of BT in India are the presence of multiple BTV serotypes, high density of ruminant and vector populations. A pentavalent inactivated, adjuvanted vaccine is administered currently in India to control BT. Recombinant vaccines with DIVA strategies are urgently needed to combat this disease. This review is the first to summarise the seroprevalence of BTV in India for 40 years, economic impact and pathobiology.

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“…Intravenous route is widely used and 100–1000 times more sensitive than yolk sac route (Pearson et al. 1985 ; Wechsler and Luedke 1991 ; Clavijo et al. 2000 ; Dadhich 2004 ).…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

“…BTV can be confirmed from culture medium by sandwich ELISA and VNT (Wechsler et al. 1990 ; Wechsler and Luedke 1991 ; Sperlova and Zendulkova 2011 ).…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

“…1970 ; St George et al. 1978 ; Sundin and Mecham 1989 ; Wechsler and Luedke 1991 ). Sometimes, BTV can also be isolated by inoculating laboratory animals, such as mice or hamsters.…”

Section: Laboratory Diagnosis Of Btmentioning

confidence: 99%

See 2 more Smart Citations

An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India

Saminathan

Singh

Khorajiya

et al. 2020

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…Blood was used for the first round of virus isolation in 10-to 12-day-old chicken embryos, according to the protocol described by Clavijo et al (29). The inoculation of embryonating chicken eggs is more sensitive than cell culture, allowing detection of BTV in samples containing a low viral concentration (30). The blood (1/10 dilution) was intravenously inoculated into an embryo vein, incubated at 33.5 ± 0.5 • C, and monitored daily for 7 days.…”

Section: Virus Isolationmentioning

confidence: 99%