Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR

Hashemi, A; Moghaddam, Mahboobeh Nakhaei; Forghanifard, Mohammad Mahdi; Yousefi, Ehsan

doi:10.52547/jommid.9.3.142

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…Finalmente, se extrajo el ADN utilizando el kit inmuPREP Bacteria DNA Kit (Analitikjena, Alemania) y se detectaron genes de resistencia blaKPC (12) , blaNDM (13) , blaIMP (14) , blaOXA-48 (15) , blaVIM (16) y mcr-1 (17) mediante Reacción en Cadena de la Polimerasa en un termociclador T100™ (Biorad, USA), siguiendo protocolos estandarizados en el laboratorio (LIBM); en el caso del gen mcr-1 se utilizó el protocolo de Iglesias (17) . Los cebadores utilizados para este proceso se detallan en la tabla 1.…”

Section: Detección De Genes Blakpc Blandm Blaimp Blaoxa-48 Blavim Y M...unclassified

Genes resistentes a carbapenémicos y colistina aislados en <i>Musca domestica</i> proveniente de un basural cercano a un hospital de Lima

Alarcón-Calle,

Osorio-Guevara,

Salas-Asencios

et al. 2024

Rev Peru Med Exp Salud Publica

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The objective was to determine the presence of genes resistant to carbapenems and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima-Peru. Isolation of carbapenemase-producing bacteria was performed in CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed by colistin discs elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM y mcr-1 genes was performed by conventional PCR. Antimicrobial susceptibility profile assessment was performed on the MicroScan automated system. Carbapenemase-producing bacteria were 31/38 and bacteria that were resistant to colistin were 26/38 with an MIC ≥ 4 μg/mL. Finally, 7 bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1) were identified.

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Section: Detección De Genes Blakpc Blandm Blaimp Blaoxa-48 Blavim Y M...unclassified

Genes resistentes a carbapenémicos y colistina aislados en <i>Musca domestica</i> proveniente de un basural cercano a un hospital de Lima

Alarcón-Calle,

Osorio-Guevara,

Salas-Asencios

et al. 2024

Rev Peru Med Exp Salud Publica

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“…ÇİD suşlarının antibiyotik direnci, belirlenen antibiyotikler kullanılarak disk difüzyon yöntemiyle doğrulanmıştır [17]. Buna göre siprofloksasin, imipenem, levofloksasin, meropenem, seftazidime dirençli PS1 ve PS3 suşları ÇİD olarak kabul edilmiştir [18,19]. PS2 ise kolistine dirençli bir suştur ve bu şekilde değerlendirmeye alınmıştır.…”

Section: Bakteri İzolasyonu Ve Kültüre Edilmesiunclassified

Antiviral İlaçlarin Hastane Enfeksiyon Etkeni Pseudomonas Aeruginosa Üzerinde Sinerjistik Etkisinin Araştirilmasi

ÖZTÜRK

2023

Ankara Ecz. Fak. Derg.

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Amaç: Yaygın hastane enfeksiyon ajanı olan Pseudomonas aeruginosa (P. aeruginosa)’ nın tedavisi için antiviral ilaçlar ile antibiyotiklerin kombinasyon olarak kullanılmaları sonucunda oluşan sinerjistik etkinliği araştırılmıştır. Gereç ve Yöntem: Antiviral ilaçların etken maddesi olan Umifenovir (UMF) ve Ribavirin (RBV) ile yaptığımız çalışmada Çok İlaca Dirençli (ÇİD) ve Kolistin (KOL) dirençli P. aeruginosa klinik izolatları kullanılmıştır. P. aeruginosa üzerinde, UMF ve RBV’ nin ayrı ayrı minimal inhibisyon katsayılarına (MİK) mikrodilüsyon yöntemi ile, KOL ve Sulbaktam (SUL) ile sinerjistik etkinliğine ise dama tahtası sinerji testi ile bakılmıştır. Sonuç ve Tartışma: Dirençli suşlarda, RBV ile KOL ve SUL kombinasyonlarında sinerji ve kısmi sinerji oluşurken (FİK = 0.375-0.75), ATCC 27853 suşu ile yapılan çalışmada indeferans ve aditif (FİK= 1.0-2.0) etkileşimin daha yoğun olduğu görülmüştür. UMF ile KOL ve SUL kombinasyonlarında ise sinerji ve kısmi sinerjiler (FİK=0.53-0.75) dikkat çekerken, ATCC 27853 suşlarında aditif (FİK=1.0) etki tespit edilmiştir. Sonuçlar değerlendirildiğinde UMF ve RBV’ nin KOL ve SUL ile kombinasyon kullanımlarının dirençli suşlar üzerinde daha etkin olduğu görülmüş olup, kombinasyonların dirençli hastane enfeksiyon etkenlerinin tedavisinde alternatif bir seçenek olarak kullanılabileceği fikrini ortaya koymuştur

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“…Pseudomonas aeruginosa is an opportunistic human pathogen capable of causing a great threat to health, especially in immunocompromised patients ) Farhan et a., 2019). It is the main cause of nosocomial infections and due to their ability to acquire resistance to a wide range of antibiotics making it a major global concern (Kumar and Illamani, 2021;Hashemi, et al, 2021; Jafari-Sales et al, 2020). It is responsible for a wide range of infections, including urinary tract, respiratory system, burn and wound infection and otitis media (Paudel et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of blaIMP-1 among P. aeruginosa isolated from patients with burn infections

Alghreri

Alsaadi²,

Al-Dahmoshi³

2022

JANS

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Carbapenems are often used as antibiotics for treating infections by multidrug-resistant Gram-negative bacteria. Pseudomonas aeruginosa is considered a major cause of health care issues associated with higher mortality and morbidity rates worldwide. This study aimed to detect the prevalence of carbapenemase gene blaIMP-1 that is produced by carbapenem-resistant P. aeruginosa isolated from burn infection patients. A total of 137 burn swabs were collected from burn patients in different hospitals in Babylon province from December 2021 to April 2022. All of the swabs were streaked on selective media cetrimide agar. Antibiotic susceptibility was performed for Imipenem and meropenem and polymerase chain reaction for species specific 16S rDNA gene and blaIMP-1 gene was done for all isolates. 50 isolates were confirmed by amplicon of species-specific 16S rDNA for P. aeruginosa. Results of antibiotic susceptibility for imipenem and meropenem revealed that 5/50 (10%) and 13/50(26%) of P. aeruginosa isolates were resistant to imipenem and meropenem, respectively. Co-susceptibility (co-resistance or co-sensitive) for both imipenem and meropenem results showed 5/50 (10%) display resistance for both imipenem and meropenem, 8/50(16%) display resistance for only meropenem while 37/50(74%) sensitive for both and none was resistant to imipenem and sensitive for meropenem. Phenotypic detection of Metallo-β-lactamase gave inverse result while the Mean±SD of inhibition zone (mm) for imipenem disc (10μg) =29.34±7.11 and for imipenem+EDTA (10 μg+750 μg) =21.8±4.98. Investigation of blaIMP-1 gene revealed that 23/50 (46%) have blaIMP-1 while 18/50 (36%) were sensitive for imipenem and meropenem but have blaIMP-1. The current study concludes the possibility of the presence of resistance gene among phenotypically sensitive isolates pushing great threat of antibiotic misuse and leaving the infection untreated.

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Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR

Cited by 4 publications

References 39 publications

Genes resistentes a carbapenémicos y colistina aislados en <i>Musca domestica</i> proveniente de un basural cercano a un hospital de Lima

Genes resistentes a carbapenémicos y colistina aislados en <i>Musca domestica</i> proveniente de un basural cercano a un hospital de Lima

Antiviral İlaçlarin Hastane Enfeksiyon Etkeni Pseudomonas Aeruginosa Üzerinde Sinerjistik Etkisinin Araştirilmasi

Prevalence of blaIMP-1 among P. aeruginosa isolated from patients with burn infections

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