Detection of bladder cancer using urinary cell‐free DNA and cellular DNA

Ou, Zhenyu; Li, Kai; Yang, Ting; Dai, Ying; Chandra, Mohan; Ning, Jun; Wang, Yongli; Xu, Ran; Gao, Tangjie; Xie, Yu; He, Qianyu; Li, Yuanwei; Qin, Lü; Wang, Long; Song, Zhuo

doi:10.1186/s40169-020-0257-2

Cited by 51 publications

(45 citation statements)

References 34 publications

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“…Furthermore, DNA methylation patterns are considered tissue-specific, so an analysis of plasma DNA methylation allows us to determine the origin of cf-DNA fragments [ 27 ]. Plasma cf-DNA methylation profiling has also been demonstrated as a very accurate approach for the diagnostics of several cancers [ 28 , 29 ]. Sequence analysis of tumor-associated cf-DNA might also be used in mutation identification.…”

Section: Cell-free Dnamentioning

confidence: 99%

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Szilágyi

Pös

Márton

et al. 2020

IJMS

127

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Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field.

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Section: Cell-free Dnamentioning

confidence: 99%

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Szilágyi

Pös

Márton

et al. 2020

IJMS

127

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“…Several studies have demonstrated that urinary cfDNA can be utilized as an important source for liquid biopsy in urologic malignant disease [5,[11][12][13][14]. Due to the increased rate of necrosis of tumor cells, urinary cfDNA carries a higher tumor genomic burden than cellular DNA [11,15]. In addition, urinary cfDNA also reflects the systemic status of patients, as it is derived from both the urinary tract and the circulatory system [16].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions

Lee

Yoon

et al. 2020

Diagnostics

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Urinary cell-free DNA (cfDNA) is an attractive body fluid for liquid biopsy. In this study, we compared the efficiencies of four commercial kits for urinary cell-free DNA (cfDNA) isolation and of various sample storage conditions. Urinary cfDNA was isolated from 10 healthy individuals using four commercial kits: QIAamp Circulating Nucleic Acid Kit (QC; Qiagen), MagMAX™ Cell-Free DNA Isolation Kit (MM; Applied Biosystems), Urine Cell-Free Circulating DNA Purification Midi Kit (NU; Norgen Biotek), and Quick-DNA™ Urine Kit (ZQ; Zymo Research). To assess the isolation efficiency, an Agilent 2100 Bioanalyzer with High Sensitivity DNA chips was used, and cfDNA yield was defined as the amount of cfDNA obtained from 1 mL of urine. MM and QC provided the highest cfDNA yield in the 50–300 bp range, and MM and NU gave the highest cfDNA yield in the 50–100 bp range. In particular, the NU kit was efficient for isolation of more fragmented cfDNA in the range of 50–100 bp with the lowest cellular genomic DNA contamination. ZQ had the best cost-efficiency for isolating the same amount of urinary cfDNA. Samples stored at −70 °C with the addition of 10 mM EDTA resulted in the highest cfDNA yield 3 months after sample collection.

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“…Lately, real-time polymerase chain reaction (PCR) has proven very effective in examining genes and DNA. Identifi cation of cell-free DNA (cfDNA) in urine samples has showed diagnostic potential in a study where fi vegene panel for urine supernatant and a seven-gene panel for urine sediments were identifi ed with promising options for identifying bladder cancer in hematuria patients (31). Long non-coding RNA (lncRNA) has been studied in association with tumorigenesis and metastasizing of bladder carcinomas.…”

Section: Discussionmentioning

confidence: 99%

The prognostic value of E-cadherin and Ki-67 compared to standard histopathologic examination in non–muscle invasive bladder cancer

Harsanyi¹,

Žiaran²,

Bevizova³

et al. 2020

BLL

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OBJECTIVES: The objectives of this study were to determine the prognostic value of expression levels of selected biomarkers and their statistical analysis in relation to survival and standard histopathologic examination and other clinicopathologic variables in non-muscle invasive bladder cancer (NMIBC). BACKGROUND: Worldwide, bladder cancer is a frequent malignant disease with rising incidence. Characteristic invasiveness and high recurrence rates call for more diagnostic methods to obtain more accurate information. Prognosis is affected by a signifi cant interpersonal variability of the disease. For this reason, constant search for alternative and better diagnostic methods is essential. METHODS: We analysed cancer tissue from patients with Ta and T1 bladder cancer. E-cadherin and Ki-67 expression levels were analysed using immunohistochemical staining. The expression levels quantifi ed to a percentual amount were statistically analysed in relation to survival and their frequency distribution in the study group. RESULTS: E-cadherin and Ki-67 expression levels show high association with tumor stage and grade (p<0.001), in contrast, the association with recurrence has proven insignifi cant. Patients with non-aberrant biomarker expression levels have much higher survival rates than the cases with aberrant expression. CONCLUSION: Low expression levels of Ki-67 and high expression levels of E-cadherin positively affect survival of patients, whereas aberrant expressions pose poorer prognosis (Tab. 2, Fig. 2, Ref. 33).

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Detection of bladder cancer using urinary cell‐free DNA and cellular DNA

Cited by 51 publications

References 34 publications

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions

The prognostic value of E-cadherin and Ki-67 compared to standard histopathologic examination in non–muscle invasive bladder cancer

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