Detection of biofilm formation ability of <i>Streptococcus agalactiae</i> isolated from bovine mastitis cases

Sohail, M. Nasim; Rathnamma, D.; Isloor, S.; Bm, Veeregowda; Sharada, R.

doi:10.5958/2250-0499.2019.00027.2

Cited by 2 publications

(4 citation statements)

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“…S. agalactiae as a mastitis pathogen which also can form biofilms [51]. The presence of pili on the surface of S. agalactiae helps in the attachment process and biofilm formation [52]. E. coli is an environmental pathogen that can cause mastitis [28].…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Kusumawati

Mustopa²,

Umami³

et al. 2022

Microbiol. Biotechnol. Lett.

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Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD 570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD 570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Kusumawati

Mustopa²,

Umami³

et al. 2022

Microbiol. Biotechnol. Lett.

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“…Highly enriched liquid or solid media are used to cultivate GBS. However, bacteria show entirely different behavior in-vivo than in vitro [3]. Sessile bacteria grown on nutrient-limited media show slow growth while planktonic bacteria multiply rapidly [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…However, bacteria show entirely different behavior in-vivo than in vitro [3]. Sessile bacteria grown on nutrient-limited media show slow growth while planktonic bacteria multiply rapidly [3,4]. Therefore, planktonic bacteria are more susceptible to antibiotics, environmental and host factors compared to its sessile biofilm [3].…”

Section: Introductionmentioning

confidence: 99%

“…Sessile bacteria grown on nutrient-limited media show slow growth while planktonic bacteria multiply rapidly [3,4]. Therefore, planktonic bacteria are more susceptible to antibiotics, environmental and host factors compared to its sessile biofilm [3]. Colonization and disease progression of bacteria is linked with biofilm formation and it is an important virulence determinant.…”

Section: Introductionmentioning

confidence: 99%

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In -vitro Biofilm Formation of Vaginal Isolates of Streptococcus agalactiae; Effect of pH and Culture Media

Dilrukshi

Kottahachchi

Dissanayake

et al. 2021

Braz. arch. biol. technol.

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Detection of biofilm formation ability of Streptococcus agalactiae isolated from bovine mastitis cases

Cited by 2 publications

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Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

In -vitro Biofilm Formation of Vaginal Isolates of Streptococcus agalactiae; Effect of pH and Culture Media

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