Detection of Bioactive Metabolites in <i>Escherichia Coli</i> Cultures Using Surface-Enhanced Raman Spectroscopy

Sun, Da‐Wen; Jayan, Heera; Pu, Hongbin

doi:10.1177/00037028221079661

Cited by 33 publications

(2 citation statements)

References 69 publications

(107 reference statements)

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“…The resulting turbidity is evidence of the ability of E. coli to move on a semisolid substrate [ 34 ]. Tryptophanase, an enzyme produced by E. coli , hydrolyzes the amino acid tryptophan into indole and pyruvic acid, causing the formation of the red ring [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

Farizqi,

Effendi,

Adikara

et al. 2023

Vet World

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Background and Aim: Escherichia coli causes a bacterial illness that frequently affects cats. Diseases caused by E. coli are treated using antibiotics. Because of their proximity to humans, cats possess an extremely high risk of contracting antibiotic resistance genes when their owners touch cat feces containing E. coli that harbor resistance genes. This study was conducted to identify multidrug-resistant E. coli and extended-spectrum β-lactamase (ESBL)-producing genes from cat rectal swabs collected at Surabaya City Veterinary Hospital to determine antibiotic sensitivity. Materials and Methods: Samples of cat rectal swabs were cultured in Brilliant Green Bile Lactose Broth medium and then streaked on eosin methylene blue agar medium for bacterial isolation, whereas Gram-staining and IMViC tests were conducted to confirm the identification results. The Kirby–Bauer diffusion test was used to determine antibiotic sensitivity, and the double-disk synergy test was used to determine ESBL-producing bacteria. Molecular detection of the genes TEM and CTX-M was performed using a polymerase chain reaction. Results: Based on morphological culture, Gram-staining, and biochemical testing, the results of sample inspection showed that of the 100 cat rectal swab samples isolated, 71 (71%) were positive for E. coli. Furthermore, 23 E. coli isolates (32.39%) demonstrated the highest resistance to ampicillin. Four isolates were confirmed to be multidurg-resistant and ESBL-producing strains. Molecular examination revealed that three E. coli isolates harbored TEM and CTX-M. Conclusion: In conclusion, pet owners must be educated on the use of antibiotics to improve their knowledge about the risks of antibiotic resistance. Keywords: cat, Escherichia coli, extended-spectrum β-lactamase, multidrug resistance, public health.

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Section: Discussionmentioning

confidence: 99%

Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

Farizqi,

Effendi,

Adikara

et al. 2023

Vet World

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“…In order to tackle food safety issues and safeguard consumer health, rapid, accurate, specific, field-deployable detection methods that meet diverse requirements are among the imperative measures for food safety assurance [18]. With their advantages of being non-destructive, rapid and comparatively low-cost, spectroscopic techniques, such as ultraviolet-visible (UV-VIS), near infrared spectroscopy (NIR) [19,20], mid-infrared (MIR) spectroscopy and raman spectroscopy [21], are widely applied in meat detection on the basis of spectral analysis [22]. Thyholt and Isaksson [23] utilized NIR diffuse reflectance spectroscopy in combination with K-nearest neighbor (KNN) to classify frozen-thawed beef.…”

Section: Introductionmentioning

confidence: 99%

Discrimination among Fresh, Frozen–Stored and Frozen–Thawed Beef Cuts by Hyperspectral Imaging

Yu,

Chen,

Zhang

et al. 2024

Foods

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The detection of the storage state of frozen meat, especially meat frozen–thawed several times, has always been important for food safety inspections. Hyperspectral imaging (HSI) is widely applied to detect the freshness and quality of meat or meat products. This study investigated the feasibility of the low-cost HSI system, combined with the chemometrics method, to classify beef cuts among fresh (F), frozen–stored (F–S), frozen–thawed three times (F–T–3) and frozen–thawed five times (F–T–5). A compact, low-cost HSI system was designed and calibrated for beef sample measurement. The classification model was developed for meat analysis with a method to distinguish fat and muscle, a CARS algorithm to extract the optimal wavelength subset and three classifiers to identify each beef cut among different freezing processes. The results demonstrated that classification models based on feature variables extracted from differentiated tissue spectra achieved better performances, with ACCs of 92.75% for PLS-DA, 97.83% for SVM and 95.03% for BP-ANN. A visualization map was proposed to provide detailed information about the changes in freshness of beef cuts after freeze–thawing. Furthermore, this study demonstrated the potential of implementing a reasonably priced HSI system in the food industry.

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Multifunctional trimetal-organic frameworks with enhanced fenton-like catalytic activity for inhibiting bacteria

Pu,

Ouyang,

Zhou

et al. 2024

Food Measure

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Antibiotics and chemical disinfectants usually have serious problems of drug resistance and biosafety. Metal-organic frameworks (MOFs), as a new alternative method, have been studied to inhibit bacteria attributed to their tunability of compositions and structures, ideal biocompatibility and great catalytic properties. In the current study, two trimetal-organic frameworks (Ni1Co1Fe1-MOFs and Ni6Co3Fe1-MOFs) with different atomic ratios of Ni/Co/Fe were synthesized by a simple one-pot room temperature method. The fabricated MOFs showed outstanding Fenton-like catalytic activities and Ni1Co1Fe1-MOFs exerted greater catalytic activity. Based on a Fenton-like reaction, a high-efficiency antibacterial system was constructed. The manufactured Ni1Co1Fe1-MOF/sodium alginate composite coating delayed the weight loss of apple chunks and effectively inhibited E. coli bacteria from polluted apple chunks after storage for 2 d, significantly improving the fruit storage. This study proposed a novel and effective antibacterial strategy, which would open up a promising idea for the exploitation of food antibacterial agents in the future.

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Detection of Bioactive Metabolites in Escherichia Coli Cultures Using Surface-Enhanced Raman Spectroscopy

Cited by 33 publications

References 69 publications

Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

Discrimination among Fresh, Frozen–Stored and Frozen–Thawed Beef Cuts by Hyperspectral Imaging

Multifunctional trimetal-organic frameworks with enhanced fenton-like catalytic activity for inhibiting bacteria

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