Detection of bioactive compounds in persimmon (Diospyros kaki) using UPLC-ESI-Orbitrap-MS/MS and fluorescence analyses

Abstract

Persimmon (Diospyros kakiL.) contains a number of metabolites, especially polyphenols and carotenoids, and persimmon is one of the most healthy fruits linking to a diet with reduced incidences of several diseases. However, little data are available on the persimmon in connection with its metabolites and properties. In recent study the differences between polar and non-polar extracts by1H NMR-based metabolomics was done. The present work presents a complete qualitative analytical characterization by UPLC-ESI-Orbit rap-MS/MS analysis and fluorescence studies in six persimmon cultivars. The metabolic differences were shown as well in the results of bioactivities and antioxidant capacities. The results of binding properties of persimmon polyphenols towards human serum albumin support polyphenolalbumin interactions with human plasma and characteristic to other plants. The presented methods can be widely used for quantitation of multiple compounds in many plants and biological samples, especially in vegetables and fruits

Pork Liver Pâté Enriched with Persimmon Coproducts: Effect of In Vitro Gastrointestinal Digestion on Its Fatty Acid and Polyphenol Profile Stability

“…Many authors have previously detected quercetin, kaempferol myricetin, and high diversity of their glycosides, being quercetin and kaempferol predominantly in persimmon fruit cv. Rojo Brillante and in other cultivars [ 25 , 48 , 52 , 53 , 54 ]. Except for quercetin glucoside III and kaempferol glucoside II, which were detected in both studied fractions, all flavonol compounds were found in persimmon flour in the free fraction.…”

“…Three of them were tentatively identified as glucosides. Monogalloyl-hexoside compounds have been mainly reported in persimmon fruit [ 48 , 51 , 54 ]. Furthermore, Maulidiani et al [ 54 ] detected two vanillin glucosides (1-O-Vanilloyl-beta-D-glucose isomer and 1-O-Vanilloyl-beta-D-glucose) in different persimmon cultivars.…”

Ameliorative effect of persimmon ( Diospyros kaki ) in cognitively impaired diabetic mice

“…The number in Figure 7 is the compound number according to Table 2. When the peaks are compared to main fragments in a previous study, they were identified as follows: 2‐O‐α‐D‐glucopyranosyl‐L‐ascorbic acid (AA‐2G) (compound 1) (Eibaid et al., 2014), monogalloyl glucose isomer (compounds 2–6) (Maulidiani et al., 2019; Wang et al., 2011), digalloyl glucose (compound 7) (Gu et al., 2013), (+)‐catechin and (‐)‐epicatechin (compounds 8 and 9) (Hofmann et al., 2016; Li et al., 2010), acteoside (compound 10) (Savarese et al., 2007), and phloretin‐3',5'‐di‐C‐β‐glucoside (compound 11) (De Beer et al., 2012).…”

From Diospyros kaki L. (Persimmon) Phytochemical Profile and Health Impact to New Product Perspectives and Waste Valorization

“…It is also known that organic acids occur naturally in food as a result of metabolic processes, namely in the metabolism of abscisic acid, and are related to the tolerance to environmental stress in plants [ 58 ], the main ones in persimmon being malic and citric acids [ 42 , 59 ]. In the studies by Senter et al with persimmons, these acids were quantified, where malic acid was the predominant acid in all cultivars studied by the authors, followed by citric [ 60 ].…”

“…Precursor ions with m/z 164, 180, and, 203 were identified as L -tyrosine, L -phenylalanine, and L -tryptophan. These authors reported the detection of precursor ions m/z 395 and 383, which they considered as peptides, with the possibility of being L -lysine, L -serine, L -tyrosine/ L -serine, L -proline, for the first and L -lysine, L -trionine, and L -histidine for the second [ 38 , 59 ].…”