Detection of Bilirubin Using Surfactant Fluorescence Enhancement and Visible Laser Fluorometry

Determination of total bilirubin in human serum by chemiluminescence from the reaction of bilirubin and peroxynitrite

“…2 showed the CL spectrum from the reaction of ONOO − with bilirubin. It is important to note that the CL spectrum of CL is similar to the fluorescence spectra of bilirubin reported in the literature [19,20], suggesting that the CL emission may be a result of a radiative transition of electrons from the first singlet excited electronic state to the ground state of bilirubin. It can be seen that the CL maximum wavelength of bilirubin is located at ca.…”

Enhancement in sample preconcentration by the on-line incorporation of cloud point extraction to flow injection analysis inside the chemiluminescence cell and the determination of total serum bilirubin

“…Therefore, an EF of nearly 1000 as obtained in the present on-line CPE/FIA system under salt-induced CPE conditions for the detection of bilirubin was likely caused by other factors in addition to an increase in bilirubin concentration within the collection column. For example, it has been shown that the fluorescence intensity of bilirubin in aqueous solutions can be improved when bound to albumin [22] or micelles [23] due to the enhanced fluorescent yield of bilirubin, which is extremely low in water at room temperature (i.e., ≤10 −5 ). When comparing the results of Experiments 2 and 4, the presence of bilirubin in a micellar solution appeared to enhance the CL intensity, especially when cotton was present.…”

Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO)

“…The skin pigments melanin and bilirubin emit fluorescence at a maximum of 436 nm (melanin) and 520-540 nm (bilirubin) [16,81]. As melanin occurs not only in the iris but also in the choroid and the RPE layer, it is of special interest when investigating FAF lifetimes.…”