Detection of beta-tubulin in the cytoplasm of the interphasic Entamoeba histolytica trophozoites

Gómez-Conde, Eduardo; Vargas, Miguel; Díaz-Orea, María Alicia; Hernández‐Rivas, Rosaura; Cárdenas-Perea, María Elena; Guerrero-González, Tayde; González-Barrios, Juan Antonio; Montiel-Jarquín, Álvaro José

doi:10.1016/j.exppara.2016.05.001

Cited by 2 publications

(3 citation statements)

References 17 publications

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“…Microtubules have also been found to contribute to phagocytosis in mammalian cells, particularly during CR3-mediated phagocytosis (Allen and Aderem, 1996;Lewkowicz et al, 2008;Newman et al, 1991). Aside from Naegleria, the only other natural system thought to lack cytoplasmic microtubules, to our knowledge, is Entamoeba histolytica ((Meza et al, 2006;Vayssie et al, 2004); but see (Gomez-Conde et al, 2016)), which move exclusively by blebbing motility (Maugis et al, 2010). Our live imaging data show that while some Naegleria cells can generate rapid bleb-like protrusions to move, they typically use phase dense, lamellar protrusions (Movie S1-S2).…”

Section: Discussionmentioning

confidence: 79%

“…For example, in keratocytes, microtubules are dispensable for motility (Euteneuer and Schliwa, 1984;Nakashima et al, 2015), while in leukocytes they mediate pathfinding and cell shape (Kopf et al, 2020;Renkawitz et al, 2019), and in fibroblasts they are required for directed migration (Liao et al, 1995;Vasiliev et al, 1970). Entamoeba histolytica are thought to lack cytoplasmic microtubules ((Meza et al, 2006;Vayssie et al, 2004); but see (Gomez-Conde et al, 2016)), and these amoebae move exclusively by blebbing (Maugis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Arp2/3 complex-mediated actin assembly drives microtubule-independent motility and phagocytosis in the evolutionarily divergent amoebaNaegleria

Fritz‐Laylin

2020

Preprint

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Much of our current understanding of actin-driven phenotypes in eukaryotes has come from the "yeast to human" opisthokont lineage, as well as the related amoebozoa. Outside of these groups lies the genus Naegleria, which diverged from humans over a billion years ago, and includes the deadly "braineating amoeba." Unlike nearly every other known eukaryotic cell type, Naegleria amoebae are thought to lack cytoplasmic microtubules. The absence of microtubules suggests that these amoebae rapidly crawl and phagocytose bacteria using actin alone. Although this makes Naegleria a powerful system to probe actin-driven functions in the absence of microtubules, surprisingly little is known about Naegleria's actin cytoskeleton. Here, we use microscopy and genomic analysis to show that Naegleria amoebae have an extensive actin cytoskeletal repertoire, complete with nucleators and nucleation promoting factors. Naegleria use this cytoskeletal machinery to generate Arp2/3-dependent lamellar protrusions, which correlate with the capacity to migrate and phagocytose bacteria. Because human cells also use Arp2/3-dependent lamellar protrusions for motility and phagocytosis, this work supports an evolutionarily ancient origin for these actin-driven processes and establishes Naegleria as a natural model system for studying microtubule-independent cytoskeletal phenotypes.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Arp2/3 complex-mediated actin assembly drives microtubule-independent motility and phagocytosis in the evolutionarily divergent amoebaNaegleria

Fritz‐Laylin

2020

Preprint

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“…2004; but see Gómez-Conde et al, 2016), and these amoebae move exclusively by blebbing (Maugis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Conserved actin machinery drives microtubule-independent motility and phagocytosis in Naegleria

Fritz‐Laylin

2020

Journal of Cell Biology

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Much of our understanding of actin-driven phenotypes in eukaryotes has come from the “yeast-to-human” opisthokont lineage and the related amoebozoa. Outside of these groups lies the genus Naegleria, which shared a common ancestor with humans >1 billion years ago and includes the “brain-eating amoeba.” Unlike nearly all other known eukaryotic cells, Naegleria amoebae lack interphase microtubules; this suggests that actin alone drives phenotypes like cell crawling and phagocytosis. Naegleria therefore represents a powerful system to probe actin-driven functions in the absence of microtubules, yet surprisingly little is known about its actin cytoskeleton. Using genomic analysis, microscopy, and molecular perturbations, we show that Naegleria encodes conserved actin nucleators and builds Arp2/3–dependent lamellar protrusions. These protrusions correlate with the capacity to migrate and eat bacteria. Because human cells also use Arp2/3–dependent lamellar protrusions for motility and phagocytosis, this work supports an evolutionarily ancient origin for these processes and establishes Naegleria as a natural model system for studying microtubule-independent cytoskeletal phenotypes.

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Detection of beta-tubulin in the cytoplasm of the interphasic Entamoeba histolytica trophozoites

Cited by 2 publications

References 17 publications

Arp2/3 complex-mediated actin assembly drives microtubule-independent motility and phagocytosis in the evolutionarily divergent amoebaNaegleria

Arp2/3 complex-mediated actin assembly drives microtubule-independent motility and phagocytosis in the evolutionarily divergent amoebaNaegleria

Conserved actin machinery drives microtubule-independent motility and phagocytosis in Naegleria

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