Detection of benzo[a]pyrene diol epoxide-DNA adducts in embryos from smoking couples: evidence for transmission by spermatozoa

Zenzes, Maria Teresa

doi:10.1093/molehr/5.2.125

Cited by 131 publications

(67 citation statements)

References 38 publications

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“…DNA adduct levels at these time points did not differ between Xpc 2/2 and Wt mice, which is logical because they both lack the ability to remove DNA damage in the period between exposure and collection. Moreover, these spermatozoa did not divide, and it is important to note that at this stage DNA damage is present until after fertilization [Zenzes et al, 1999b]. Mutations may be induced after DNA replication resumes which, of course, holds for all DNA adducts present in any mature spermatozoa.…”

Section: Discussionmentioning

confidence: 96%

DNA adduct kinetics in reproductive tissues of DNA repair proficient and deficient male mice after oral exposure to benzo(a)pyrene

Verhofstad

Oostrom

Benthem

et al. 2009

Environ and Mol Mutagen

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Benzo(a)pyrene (B[a]P) can induce somatic mutations, whereas its potential to induce germ cell mutations is unclear. There is circumstantial evidence that paternal exposure to B[a]P can result in germ cell mutations. Since DNA adducts are thought to be a prerequisite for B[a]P induced mutations, we studied DNA adduct kinetics by 32 P-postlabeling in sperm, testes and lung tissues of male mice after a single exposure to B[a]P (13 mg/kg bw, by gavage). To investigate DNA adduct formation at different stages of spermatogenesis, mice were sacrificed at Day 1, 4, 7, 10, 14, 21, 32, and 42 after exposure. In addition, DNA repair deficient (Xpc 2/2 ) mice were used to study the contribution of nucleotide excision repair in DNA damage removal. DNA adducts were detectable with highest levels in lung followed by sperm and testis. Maximum adduct levels in the lung and testis were observed at Day 1 after exposure, while adduct levels in sperm reached maximum levels at 1 week after exposure. Lung tissue and testis of Xpc 2/2 mice contained significantly higher DNA adduct levels compared to wild type (Wt) mice over the entire 42 day observation period (P < 0.05). Differences in adduct half-life between Xpc 2/2 and Wt mice were only observed in testis. In sperm, DNA adduct levels were significantly higher in Xpc 2/2 mice than in Wt mice only at Day 42 after exposure (P 5 0.01). These results indicate that spermatogonia and testes are susceptible for the induction of DNA damage and rely on nucleotide excision repair for maintaining their genetic integrity. Environ. Mol. Mutagen. 51:123-129, 2010. V V C 2009 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 96%

DNA adduct kinetics in reproductive tissues of DNA repair proficient and deficient male mice after oral exposure to benzo(a)pyrene

Verhofstad

Oostrom

Benthem

et al. 2009

Environ and Mol Mutagen

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“…in fathers who smoke, can be passed on to their children and has been linked with an increased incidence of childhood cancer in their offspring (Zenzes et al 1999, Zenzes 2000. This has caused concern over the use of sperm from subfertile men in assisted reproductive technologies (IVF and ICSI).…”

Section: Discussionmentioning

confidence: 99%

“…DNA damage rates in sperm are higher in infertile individuals (Irvine et al 2000), patients subjected to cancer therapies (Charak et al 1990, Kenney et al 2001, men who smoke (Zenzes et al 1999, Zenzes 2000 and also possibly in older men (Wyrobek et al 2006). Concerns have been expressed that using sperm with damaged DNA for intracytoplasmic sperm injection (ICSI) could have a long-term effect on the health of the children born to subfertile couples (Gosden et al 2003).…”

Section: Introductionmentioning

confidence: 99%

A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice

Paul¹,

Murray²,

Spears³

et al. 2008

Reproduction

230

168

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Infertility represents a major clinical problem and 50% of cases are attributable to the male partner. Testicular function is temperature dependent, and in both man and mouse the position of the testes in the scrotum ensures that they are kept at between 2 and 8 8C below core body temperature. We used a mouse model to investigate the impact of a single, transient, mild, scrotal heat stress (38, 40 or 42 8C for 30 min) on testicular function, sperm DNA integrity and embryo survival. We detected temperature-dependent changes in testicular architecture, number of apoptotic cells and a significant reduction in testis weight 7 and 14 days after heat stress at 42 8C. We report for the first time that DNA strand breaks (g-H2AX-positive foci) were present in spermatocytes recovered from testes subjected to 40 or 42 8C. Fertility of heat-stressed males was tested 23-28 d after treatment (sperm at this time would have been spermatocytes at time of heating). Paternal heat stress at 42 8C resulted in reduced pregnancy rate, placental weight and litter size; pregnancies from the 40 8C group had increased resorptions at e14.5. Abnormalities in embryonic development were detected at e3.5 and in vitro fertilisation with sperm recovered 16 h or 23 d after scrotal stress at 42 8C revealed a block in development between the 4-cell and blastocyst stages. This study has provided evidence of temperature-dependent effects on germ cell DNA integrity and highlighted the importance of an intact paternal genome for normal embryo development. Reproduction (2008) 136 73-84

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“…Benzo[a]pyrene (B[a]P) is a potent carcinogen in cigarette smoke. Its reactive metabolite adducts with DNA, which can result in mutations [113]. This can result in both male and female infertility.…”

Section: Life Stylementioning

confidence: 99%

Male Infertility; Evidences, Risk Factors, Causes, Diagnosis and Management in Human

Sharma¹

2017

Ann Clin Lab Res

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Infertility has become ominous problem. On an average, about 10% of all couples face difficulty in starting a family and this creates a feeling of great personal failure, particularly in India where religious and socio-economic traditions have made it almost imperative for everyone to have children. A significant association had been found between impaired semen quality including sperm count, motility and morphology. In this review, the various contributory etiological factors i.e., exposure to heavy metals, pesticides, industrial chemicals, endocrine factors, genetics causes and modern life style had been discussed which have a serious impact on male infertility. In this article we analyzed data from different sources and present evidences of the possible etiology and risk factors for male infertility. There is a need to emerge at the indiscriminate use and disposal of environmental chemicals. Especially pesticides and industrial chemicals as the chemicals enter the food chain, surface and ground water which had potential for exposure during the critical period of development further avoiding tobacco smoking, excessive alcoholism, excessive heat exposure to the testes can help in improving the semen quality.

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Detection of benzo[a]pyrene diol epoxide-DNA adducts in embryos from smoking couples: evidence for transmission by spermatozoa

Cited by 131 publications

References 38 publications

DNA adduct kinetics in reproductive tissues of DNA repair proficient and deficient male mice after oral exposure to benzo(a)pyrene

DNA adduct kinetics in reproductive tissues of DNA repair proficient and deficient male mice after oral exposure to benzo(a)pyrene

A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice

Male Infertility; Evidences, Risk Factors, Causes, Diagnosis and Management in Human

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