Detection of Balenine in Mouse Plasma after Administration of Opah-Derived Balenine by HPLC with PITC Pre-Column Derivatization

Abstract

We examined the absorption of balenine (Bal) in mouse blood after the administration of a high-purity Bal prepared from opah muscle. Using HPLC with phenyl isothiocyanate pre-column derivatization, we successfully isolated imidazole peptides and their constituents. We detected Bal and 3-methylhistidine (3-Me-His) in mouse blood 1 h after the administration of opah-derived Bal. The concentrations of Bal and 3-Me-His significantly increased to 128.27 and 69.09 nmol/mL in plasma, respectively, but were undetectable in control and carnosine (Car)-administrated mice. In contrast, β-alanine and histidine did not increase in mouse plasma 1 h after the administration of Car and opah-derived Bal. The present study is the first report on the absorption of food-derived Bal in mouse blood and serves as a pilot study for future clinical trials.

Balenine, Imidazole Dipeptide Promotes Skeletal Muscle Regeneration by Regulating Phagocytosis Properties of Immune Cells

“…Sigemura et al demonstrated that balenine was detected in the plasma of mice at 1 h after oral administration of opah-derived balenine [ 36 ]. Notably, the concentration of balenine in plasma was higher than that of carnosine.…”

“…They were given a 1% w / w concentration of balenine-enriched extract diet (the concentration of balenine described in the previous study) or a normal diet for two weeks, from seven weeks of age. Shigemura et al reported that the administration of 200 mg/kg opah-derived balenine did not affect endogenous amino acid concentrations, including histidine and alanine in the blood [ 36 ]. The nutritional composition of each diet was the same as in a previous mice experiment [ 41 ].…”

Anserine, a Histidine-Containing Dipeptide, Suppresses Pressure Overload-Induced Systolic Dysfunction by Inhibiting Histone Acetyltransferase Activity of p300 in Mice

“…Anserine content was measured in heart tissue according to the method of Shigemura et al [41] with some modifications. In brief, heart tissue (100 mg) was added in phosphatebuffered saline and homogenized using Micro Smash TM MS-100R at 5000 rpm, at 4 • C and for 80 s. After centrifugation at 12,000× g, at 4 • C and for 10 min, the supernatant was added trichloroacetic acid (5%), vortexed, centrifugated at 12,000× g, at 4 • C and for 10 min and filtrated through a 0.45 µm hydrophilic nylon membrane.…”