Detection of bacteriocin production and virulence traits in vancomycin-resistant enterococci of different sources

Sabia, Carla; Niederhäusern, Simona De; Guerrieri, Elisa; Messi, Patrizia; Anacarso, Immacolata; Manicardi, Giuliano; Bondi, Moreno

doi:10.1111/j.1365-2672.2007.03612.x

Cited by 44 publications

(38 citation statements)

References 61 publications

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“…However, recent investigation described different incidences in clinical isolates; for instance, Sabia et al reported that 70% of vancomycin resistance enterococci isolates carried the gelE; they also found gelatinase activity in E. faecalis species more than E. faecium. Nevertheless, Sharifi et al detected gelE with 7.9% of vancomycin resistance enterococci in Hospitalized Patients of Northwest of Iran (17,22). Previous studies have indicated the importance and frequency of gelatinase enzyme in enterococcal infections.…”

Section: Discussionmentioning

confidence: 99%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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Background: The enterococci are responsible for infections including bacteremia and endocarditis which are usually resistant to multiple antibiotics. This nosocomial agent probably harbors putative virulence factors which increases their capability to colonize hospitalized patients. Objectives: This study was aimed in order to find the frequency of various virulence factors in enterococci and their relationship with multidrug resistance (MDR). Methods: The samples were collected from different hospital wards including; Intensive care unit (ICU), cardiac care unit (CCU), pediatrics department, internal wards, and transplantation. The isolates were detected by biochemical tests and in order to determine the antibiotic susceptibility pattern, disk diffusion agar (kirby-bauer) was accomplished. Then, MICs (Minimum inhibitory concentrations) of vancomycin were determined by E-test strips. For molecular examinations and detection of drug resistance genes, the simple polymerase chain reaction was used. The multiplex PCR was used in order to detect virulence factors. Results: Total of 85 isolates were obtained from one university teaching hospital in southeast of Iran. Approximately 95% of isolated which were from urine specimens and 34% of isolates were collected from pediatrics units at hospital. Tetracycline resistance (48%) has been observed with a high frequency and related to the tetM gene. Furthermore, eighteen isolates were recognized as MDR strains that carried vanA, aac (6)-Ie-aph (2)-Ia, ermB, and tetM genes. Among virulence factor genes, asa1 (69%) and gelE (55%) are more frequently observed in both species. In general, we found Enterococcus faecalis strains more prevalent. Also, E. faecium was related to antibiotic resistance genes in nosocomial infection. Conclusions:The data was indicated a high prevalence of multiple antibiotics resistance genes with virulence determinants in enterococci and also considered resistant isolate in pediatrics unit. The current results can be recommended in order to change new strategies for antibiotic therapy, because this serious pathogen is important for treatment and eradication in hospitals. Furthermore, the biofilm formation was regulated and constructed by virulence determinants that could be a candidate for enterococcal treatment.

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Section: Discussionmentioning

confidence: 99%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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“…Although a screening for production of bacteriocins by E. faecalis and E. faecium was the subject of several previous studies [10][11][12][13][14], only little information is available about this feature in E. avium, E. hirae, E. mundtii, E. casseliflavus and E. durans isolated from different environments. The aim of this study was to screen the production of antimicrobial activities as well as the presence of bacteriocin structural genes in enterococcal isolates include diverse species isolated from different sources in Turkey.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

et al. 2011

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A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E. casseliflavus (4), E. durans (4), E. faecalis (4), E. mundtii (3) and E. avium (2). Of the 57 isolates 40 of them inhibited the growth of at least one indicator bacterium. Based on our PCR results 54 strains possesed enterocin genes. The genes of entA and entB were the most frequently detected structural genes among the PCR positive strains (54 and 53 strains, respectively) and the entB gene was always associated with entA gene. The highest combination of enterocin genes (24 of 54 strains) detected was entA, entB, entP and entL50A/B. The enterocins AS-48 and CylL LS genes were not found. Three enterococcal isolates, 2 E. faecium and 1 E. hirae were not harbour any of tested enterocin genes. No correlation between the presence of enterocin structural genes and the origin of the strain was detected, also no relationship seemed to exist between the tested enterocin genes and the activity spectra of isolates. Genes encoding bacteriocins are widely disseminated among enterocci from different origin and more studies should be done for evaluate industrial potential of bacteriocins.

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“…Sin embargo, las especies vinculadas a patologías humanas quedan limitadas a E. faecalis y E. faecium mientras que las restantes resultan agentes etiológicos infrecuentes (Sabia et al, 2008).…”

Section: Introductionunclassified

Efecto de las condiciones de crecimiento y composición del medio de cultivo sobre la producción de bacteriocina de Enterococcus mundtii Tw56

Vallejo¹,

Ledesma²,

Anselmino³

et al. 2014

Rev. Colomb. Biotecnol.

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ResumenEnterococcus mundtii Tw56 es una cepa productora de bacteriocina que fue aislada del contenido intestinal de pejerrey (Odontesthes sp.). El objetivo del presente trabajo fue determinar los factores fisicoquímicos y la composición del medio de cultivo para lograr un mayor rendimiento de células viables y producción de bacteriocina. No se observaron cambios en la producción del antimicrobiano cuando la glucosa fue sustituida por fructosa o maltosa en la formulación del medio MRS. Por el contrario, la mayor actividad de las bacteriocinas se obtuvo cuando se utilizó el extracto de carne como fuente única de nitrógeno. Mientras que la máxima biomasa se alcanzó a 35 °C, las temperaturas óptimas para la producción de bacteriocina se observaron a 25 y 30 °C. El pH inicial óptimo para el crecimiento celular y bioactividad fue 6,5, ambos parámetros disminuyeron cuando la experiencia comenzó a pH 6,0 o 5,5. La formación de biomasa y la producción de bacteriocina disminuyeron en presencia de cloruro de sodio. La cepa comenzó a producir bacteriocina en la fase exponencial tardía. La actividad aumentó en función de la masa celular y alcanzó el máximo al final de la fase exponencial (12 h). Una disminución de la actividad antimicrobiana se observó en la fase estacionaria (16 h), posiblemente debido a la degradación por enzimas proteolíticas. Palabras clave:Enterococcus mundtii Tw56, bacteriocina, factores fisicoquímicos, medio de cultivo. AbstractEnterococcus mundtii Tw56 is a bacteriocin-producing strain that was isolated from intestinal content of silverside (Odontesthes sp.). The aim of the present work was to determine physicochemical factors and culture medium composition for higher yield of viable cells and bacteriocin production. No changes were observed in the antimicrobial production when glucose was replaced by fructose or maltose in the MRS medium formulation. On the other hand, highest bacteriocin activity was obtained when meat extract was used as a sole nitrogen source. While the maximun biomass was achieved at 35 ºC, the optimal temperatures for bacteriocin production were observed at 25 and 30 ºC. The optimal initial pH for cell growth and bioactivity was 6.5, both parameters dropped when the experience started at pH 6.0 or 5.5. Biomass formation and bacteriocin production decreased in the presence of sodium chloride. The strain started producing the bacteriocin at the late exponential phase. The activity increased as a function of the cell mass and reached the maximun at the end of exponential phase (12 h). A decrease of antimicrobial activity was observed in the stationary phase (16 h), possibly due to degradation by proteolitic enzimes.

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Detection of bacteriocin production and virulence traits in vancomycin-resistant enterococci of different sources

Cited by 44 publications

References 61 publications

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Efecto de las condiciones de crecimiento y composición del medio de cultivo sobre la producción de bacteriocina de Enterococcus mundtii Tw56

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