Detection of Bacterial Spores with Lanthanide−Macrocycle Binary Complexes

Abstract

The detection of bacterial spores via dipicolinate-triggered lanthanide luminescence has been improved in terms of detection limit, stability, and susceptibility to interferents by use of lanthanidemacrocycle binary complexes. Specifically, we compared the effectiveness of Sm, Eu, Tb and Dy complexes with the macrocycle 1, 4,7, to the corresponding lanthanide aquo ions. The Ln(DO2A) + binary complexes bind dipicolinic acid (DPA), a major constituent of bacterial spores, with greater affinity and demonstrate significant improvement in bacterial spore detection. Of the four luminescent lanthanides studied, the terbium complex exhibits the greatest dipicolinate binding affinity (100-fold greater than Tb 3+ alone, and 10-fold greater than other Ln(DO2A) + complexes) and highest quantum yield. Moreover, the inclusion of DO2A extends the pH range over which Tb-DPA coordination is stable, reduces the interference of calcium ions nearly 5-fold, and mitigates phosphate interference 1000-fold compared to free terbium alone. In addition, detection of Bacillus atrophaeus bacterial spores was improved by the use of Tb (DO2A) + , yielding a 3-fold increase in the signal-to-noise ratio over Tb 3+ . Out of the eight cases investigated, the Tb(DO2A) + binary complex is best for the detection of bacterial spores.

Crystal structure of (2,6-pyridinedicarboxylato-κ3O,N,O')[2,6-bis(benzimidazol- 2-yl)pyridine-κ3N,N',N'']copper(II)— methanol (1:1), C27H20CuN6O5

“…2,6-Pyridinedicarboxylate is one of the frequently used aromatic dicarboxylates as the key component of bacterial spores [2]. Metal complexes with 2,6-pyridinedicarboxylate ligands own interesting structural features with various coordination modes and strong H-bonds for self-assembly [3,4] and the ligand has been used as a coligand in constructing magnetic coupling systems [5].…”

Beetroot-Pigment-Derived Colorimetric Sensor for Detection of Calcium Dipicolinate in Bacterial Spores

“…Endospores also release CaDPA upon thermal treatment or in the presence of reactivation agents that induce bacterial germination, such as inosine and L-alanine [10]. Although there are several methods for the detection of B. anthracis [11], [12], the simple and fast detection of dipicolinic acid (DPA) using luminescent lanthanide has been promising [9], [13], [14], [15].…”

Covalently bonded Ln(III) hybrid materials showing dual properties of visible to near infrared photoluminescence and gas adsorption

“…Lanthanide complexes have been attracting much attention in recent years due to their ability to emit intense and characteristic photoluminescence based on the "antenna effect" of organic ligands [1][2][3][4][5]. However, due to the poor mechanical, thermal and soluble properties, their potential applications in such fields as OLED, chemosensor and bio-assays cannot be fully applied.…”

“…The coordination of N atoms on Si-CNTB with Ln(III) ions afforded efficient luminescent centers and the co-hydrolyzation of silane groups with TEOS (tetraethoxysilane) produced covalently linked hybrid particles in sub-micron scale. Visible to near infrared photoluminescence and N 2 adsorption properties were observed in this new type of inorganic-organic hybrid materials.Lanthanide complexes have been attracting much attention in recent years due to their ability to emit intense and characteristic photoluminescence based on the "antenna effect" of organic ligands [1][2][3][4][5]. However, due to the poor mechanical, thermal and soluble properties, their potential applications in such fields as OLED, chemosensor and bio-assays cannot be fully applied.…”