Detection of Bacterial Quorum Sensing Molecules

Stein, Elke; Schikora, Adam

doi:10.1007/978-1-4939-7604-1_14

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…QQ activities could be promising as a prospective tool to improve plant health and bypass antibiotic resistance in the development of biological products combating plant pathogens ( Grandclément et al, 2016 ; Rodríguez et al, 2020 ). Many screening techniques have been utilized to identify QQ microbial isolates for this purpose ( Tang et al, 2013 ; Last et al, 2016 ; Stein and Schikora, 2018 ). To better understand the capacity of UD1022 for QQ, we used the bioreporter strain Agrobacterium tumefaciens KYC55 in soft agar to detect both short- and long-chain AHLs.…”

Section: Discussionmentioning

confidence: 99%

Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula

2021

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Plant growth-promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a “consortia” approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530, and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth-promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model “consortium.” These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula

2021

View full text Add to dashboard Cite

show abstract

“…QQ activities could be promising as a prospective tool to improve plant health and bypass antibiotic resistance in the development of biological products combating plant pathogens (Grandclément et al, 2016; Rodríguez et al, 2020). Many screening techniques have been utilized to identify QQ microbial isolates for this purpose (Tang et al, 2013; Last et al, 2016; Stein and Schikora, 2018). To better understand the capacity of UD1022 for QQ, we used the bioreporter strain Agrobacterium tumefaciens KYC55 in soft-agar to detect both short and long-chain AHLs.…”

Section: Discussionmentioning

confidence: 99%

Quorum quenching activity of the PGPRBacillus subtilisUD1022 alters nodulation efficiency ofSinorhizobium melilotionMedicago truncatula

Rosier

Beauregard

Bais

2020

Preprint

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Plant growth promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a ‘consortia’ approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530 and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model ‘consortium’. These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.

show abstract

Culture-Dependent and Culture-Independent Analyses of the Effects of Signal Compounds on Microbial Community Dynamics in Lake Sediment

Zhou

Zhang

et al. 2021

Environmental Engineering Science

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Detection of Bacterial Quorum Sensing Molecules

Cited by 5 publications

References 15 publications

Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula

Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula

Quorum quenching activity of the PGPRBacillus subtilisUD1022 alters nodulation efficiency ofSinorhizobium melilotionMedicago truncatula

Culture-Dependent and Culture-Independent Analyses of the Effects of Signal Compounds on Microbial Community Dynamics in Lake Sediment

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